Caraka Tani: Journal of Sustainable Agriculture
Vol 36, No 2 (2021): October

Banana Cultivars Microshoot Induction and Plantlet Formation Using Cytokinin and Auxin

Sugiyono Sugiyono (Department of Botany, Faculty of Biology, Universitas Jenderal Soedirman, Purwokerto)
Prita Sari Dewi (Department of Agrotechnology, Faculty of Agriculture, Universitas Jenderal Soedirman, Purwokerto)
Rendie Prasetyo (Department of Botany, Faculty of Biology, Universitas Jenderal Soedirman, Purwokerto)



Article Info

Publish Date
06 Jun 2021

Abstract

Banana is a horticultural plant with very high potentials, which contains carbohydrates and vitamins that are useful in fulfilling people's food and nutritional needs. Hence, this study aims to produce superior banana seedlings and develop a protocol for their mass production using a plant in vitro culture technique. This was a two stage-experiment i.e. microshoot production and plantlet formation. The result showed that Gebyar cultivar produced more shoots than the Kepok Kuning cultivar, with an average of 4.25 microshoots explant-1. However, Kepok Kuning produced more leaves than Gebyar, with an average of 4.64 leaves plantlet-1. Banana shoots cultured on the media containing Indole-3-acetic acid (IAA) at a concentration of 2.5 µM produced the highest leaves number. Meanwhile, those cultured on the media containing 1-Naphthalenesacetic acid (NAA) at a concentration of 7.5 µM produced the highest roots number. A Murashige and Skoog (MS) medium supplemented with 6-Benzylaminopurine (BAP) up to 30 µM and the one supplemented with 7.5 μM of NAA are suitable for Kepok Kuning and Gebyar cultivars micropropagation with regard to microshoot induction and plantlet formation, respectively.

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Journal Info

Abbrev

carakatani

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Caraka Tani: Journal of Sustainable Agriculture publishes original articles, review articles, case studies and short communications on the fundamentals, applications and management of Sustainable Agriculture areas in collaboration with Indonesian Agrotechnology / Agroecotechnology Association ...