Microbiology Indonesia
Vol. 6 No. 4 (2012): December 2012

Bacteria Associated with Arbuscula Mycorrhizal Spores Gigaspora margarita and Their Potential for Stimulating Root Mycorrhizal Colonization and Neem (Melia azedarach Linn) Seedling Growth

SRI WILARSO BUDI (Department of Silviculture Faculty of Forestry, Institut Pertanian Bogor , Campus IPB Darmaga. Bogor)
YENNI BAKHTIAR (Center of Biotechnology, Badan Pengkajian dan Penerapan Teknologi, Gedung 630 Kawasan Puspitek Serpong Tangerang Selatan 15314, Indonesia)
NUNANG LAMAEK MAY (Faculty of Forestry, Universitas Cendrawasih, Jalan Camp Wolker , Jayapura 99358, Papua, Indonesia)

Article Info

Publish Date
19 Apr 2013

Abstract

Four isolates (BGi1, BGi2, BGi3, and BGi4) bacteria were isolated from surface sterilized arbuscular mycorrhizal fungi (AMF) spores of Gigaspora margarita (Gm). Based on 16S rDNA analyses and phylogenetic tree, it was revealed that isolates BGi1, BGi3 and BGi4 belong to genus Bacillus, whereas BGi2 was very close  to Bacillus megaterium EG 24. Enzymatic activity test showed that all four isolates had cellulase and protease activities; while one isolate (Bacillus sp. BGi4) also has pectinase activity in addition to the celulase  and protease activities. Dual inoculation of Melia azedarch Linn roots by B. megaterium BGi2  and AMF spores G. margarita enhanced mycorrhizal root colonization by 58.3 %. Combination of Bacillus sp. BGi1 and G. margarita increased  height, diameter, shoot biomass, and root biomass of  M. azedarch by  353, 4.8, 4546, and 2810%, respectively,  in comparison to the uninoculated control plant.

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Journal Info
Microbiology Indonesia
Website

Abbrev

mionline

Publisher

Perhimpunan Mikrobiologi Indonesia

Subject

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

Description

Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, ...