Budapest International Research in Exact Sciences (BirEx Journal)
Vol 4, No 2 (2022): Budapest International Research in Exact Sciences, April

Detection of Enterobacteriaceae producing Extended Spectrum -Lactamases (ESBL) in Beef Cattle Sold at the Animal Market in Central Lombok

Dian Neni Naelasari (Nutrition Science Study Program, Universitas Nahdlatul Ulama Nusa Tenggara Barat, Indonesia)
Febrina Sulistiawati (Nutrition Science Study Program, Universitas Nahdlatul Ulama Nusa Tenggara Barat, Indonesia)



Article Info

Publish Date
13 Apr 2022

Abstract

The Province of West Nusa Tenggara Province (NTB) is known as a source of beef cattle in eastern Indonesia. The use of antibiotics in animal foods can threaten health and cause antibiotic resistance to the normal flora of the animal and humans. One of the causes of antibiotic resistance is extended spectrum β-lactamase (ESBL). The purpose of the study was to detect the prevalence of ESBL-producing Enterobacteriaceae in beef cattle sold in the central Lombok animal market. The faecal samples were collected from beef cattle to 65. Cultured on MacConkey agar supplemented with cefotaxim 1 mg/L, incubated at 37oC for 24 hours. Then the growing colony were tested for ESBL producer by Double Disk Synergy Test (DDST). Identify the type of bacteria by biochemical testing. Identify the type of bacteria by biochemical testing and gram staining. Results: identified showed 1 (1.5%) sample of ESBL positive beef cattle the faecal samples with Escherichia coli bacteria (100%). Through feces, resistant bacteria contained in animal faecal can migrate to the environment and cross-transmission of genetic material between bacteria through water, soil, air, plants, food, humans and food-producing animals.

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Journal Info

Abbrev

birex

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Physics Transportation

Description

Budapest International Research in Exact Sciences (BirEx-Journal) is a peer reviewed journal published in January, April, July, October welcome research paper in Medical Science, Agriculture Science, Biological Science, Engineering Science and other related areas and it is published in both online ...