Acta Chimica Asiana
Vol. 5 No. 1 (2022)

Antibacterial Test and Isolation of Xanthones from Pericarps Mangosteen (Garcinia mangostana L.) using Calcium Oxide (CaO) as a Vacuum Liquid Chromatography (VLC) Stationary Phase

Ni Komang Tri Dharmayani (Universitas Mataram)
Inggit Garnasih (Chemistry Department, Faculty of Mathematics and Natural Sciences, University of Mataram, Indonesia)
Emmy Yuanita (Chemistry Department, Faculty of Mathematics and Natural Sciences, University of Mataram)
Maria Ulfa (Chemistry Department, Faculty of Mathematics and Natural Sciences, University of Mataram)
Sudirman Sudirman (Chemistry Department, Faculty of Mathematics and Natural Sciences, University of Mataram)
Made Ganesh Darmayanti (School of chemistry Monash University, Australia)
Maulida Septiyana (School of chemistry The University of Edinburgh, Scotland)



Article Info

Publish Date
28 Apr 2022

Abstract

Xanthones are one of the biggest classes of compounds in natural product chemistry. Xanthones have been isolated from pericarp mangosteen (Garcinia mangostana L.) and then purified with VLC using calcium oxide (CaO) as the stationary phase and eluted using n-hexane: EtOAc of increasing polarity. Extraction of the pericarp mangosteen using the maceration method with acetone as the solvent. The isolation of xanthones was carried out through various chromatography techniques, such as vacuum liquid chromatography (VLC), gravitational column chromatography (GCC), and thin layer chromatography (TLC). The molecular structure of the compound was identified based on spectroscopy data, including IR, 1D NMR (1H and 13C), and 2D NMR (HSQC and HMBC). Based on the analysis of spectrum data, the isolated compound is ɑ-mangostin. The acetone extract and ɑ-mangostin were also examined for antibacterial activity against Gram positive (+) Staphylococcus aureus (S. aureus) ATCC 25923 and Gram negative (-) Escherichia coli (E. coli) ATCC 25922. The largest inhibition zone was shown by ɑ-mangostin against S. aureus (12.16 mm) more than E. coli (12 mm).

Copyrights © 2022






Journal Info

Abbrev

ACA

Publisher

Subject

Chemistry

Description

Acta Chimica Asiana (online ISSN 2550-0503, print ISSN 2550-049x) is a peer-reviewed, open access journal that publishes high-quality original research articles as well as review articles in all areas of Chemistry and Chemical Engineering. Significant finding that is insufficient to be presented as ...