Makara Journal of Science
Vol. 22, No. 1

Optimization of Laccase Adsorption-Desorption Behaviors on Multi-Walled Carbon Nanotubes for Enzymatic Biocathodes

Suherman, Alex Lukmanto (Unknown)
Zebda, Abdelkader (Unknown)
Martin, Donald K. (Unknown)

Article Info

Publish Date
20 Mar 2018


Laccase adsorption-desorption behaviors on the surface of multi-walled carbon nanotubes (MWCNTs) were investi-gated using spectrophotometry and voltammetry. The optimum condition for laccase adsorption is 5.0 mg/mL of laccase in 0.01 M phosphate-buffered saline (PBS) at pH 5.0. Laccase adsorption is a reversible phenomenon that is dependent upon the nature of MWCNTs and the concentration of ionic strength in the laccase solution. Chitosan was functiona-lized as a nanoporous reservoir to minimize laccase desorption. Chitosan was found to protect approximately 97.2% of the adsorbed laccase from MWCNTs during the first six hours of observation. The three-dimensional (3D) biocathode, MWCNTs-laccase-chitosan with a 0.2 cm2 geometric area, was shown to have a stable open circuit potential (OCP) of 0.55 V, a current density of 0.33 mA cm-2 at 0.2 V vs. saturated calomel electrode (SCE), and a stable current for 20 hours of successive measurements. This report provides a new insight into the study of a high-performance laccase-based biocathode via optimization of adsorption and minimization of desorption phenomena.

Copyrights © 2018

Journal Info






Makara Journal of Science publishes original research or theoretical papers, notes, and minireviews on new knowledge and research or research applications on current issues in basic sciences, namely: Material Sciences (including: physics, biology, and chemistry); Biochemistry, Genetics, and ...