Jurnal Sain Peternakan Indonesia
Vol 17 No 3 (2022)

Polymorphism of Prolactin Gene (PRL/PstI) In Sikumbang Jonti Duck Using PCR-RFLP Methods

Teguh Rafian (Universitas Lampung)
Yurnalis Yurnalis (Universitas Andalas)
Yosi Fenita (Universitas Bengkulu)
Rafi Iskandarsyah (Universitas Andalas)



Article Info

Publish Date
29 Sep 2022

Abstract

This study is aimed to determine polymorphism of the Prolactin gene (PRL|PstI) in Sikumbang Jonti ducks using PCR-RFLP (Polymerase Chain Reaction–Restriction Fragment Length Polymorphism) method. This study used 56 Sikumbang Jonti ducks whose blood samples were taken. Gene amplification used a pair of primers forward 5' TGC AAA CCA TAA AAG AAA AGA 3' and reverse 5' CAA TGA AAA GTG GCA AAG CAA 3', which resulted in a 400 bp fragment in exon 5 of the Prolactin (PRL) gene. The amplification product was restricted using the PstI enzyme, which recognizes the truncation site (5' G↓ACGTC 3'). From 56 samples of Sikumbang Jonti ducks identified, just one genotype was found, homozygous (-/-) with only one allele (-). Analysis of the restriction product in Sikumbang Jonti ducks obtained a uniform genetic variation of PRL|PstI (monomorphic) with an allele frequency (-) of 100%.

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Journal Info

Abbrev

jspi

Publisher

Subject

Veterinary

Description

Jurnal Sain Peternakan Indonesia (JSPI) pISSN 1978 – 3000 dan eISSN 2528 – 7109 adalah majalah ilmiah resmi yang dikeluarkan oleh Jurusan Peternakan Fakultas Pertanian Universitas Bengkulu, sebagai sumbangannya kepada pengembangan Ilmu Peternakan yang diterbitkan dalam Bahasa Indonesia ...