HAYATI Journal of Biosciences
Vol. 30 No. 1 (2023): January 2023

Anti-Inflammatory Effect of Isolate Compounds from Ethyl Acetate Fraction of Piper crocatum Ruiz and Pav Leaves on Lipopolysaccharide-induced RAW 264.7 Cells

Nilda Lely (Postgraduate Student, Faculty of Pharmacy, Universitas Andalas, Padang, Indonesia. STIFI Bhakti Pertiwi, Palembang, Indonesia)
. Dachriyanus (Faculty of Pharmacy, Andalas University, Padang, Indonesia)
Yufi Aldi (Faculty of Pharmacy, Andalas University, Padang, Indonesia)
. Almahdy (Faculty of Pharmacy, Andalas University, Padang, Indonesia)
Fatma Sri Wahyuni (Faculty of Pharmacy, Andalas University, Padang, Indonesia)

Article Info

Publish Date
19 Aug 2022

Abstract

Red betel (Piper crocatum Ruiz and Pav) is a traditional Indonesian plant. The ethyl acetate fraction of red betel leaf has the potential as an anti-inflammatory. This research aimed to determine the anti-inflammatory effect of isolate compound from ethyl acetate fraction of Piper crocatum leaves on the production of cytokine pro-inflammatory TNF-α and ICAM-1 in lipopolysaccharide-induced RAW 264.7 cells. Cell viability was determined by the MTT method in the concentration range of 1.25; 2.5; 5; 10; 20, and 40 µg/ml. The TNF-α and ICAM-1 level was determined by the TNF-α and ICAM-1 assay kit as measured by an ELISA plate reader. Cell viability test of isolate compound from ethyl acetate fraction, the concentrations that gave viability percentages above 80% were 10; 5; 2.5 and 1.25 ug/ml. Isolate compound at concentrations of 10, 5, 2.5 and 1.25 ug/ml significantly inhibited TNF-α and ICAM-1 production with p<0.05 (p = 0.000).

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Journal Info
HAYATI Journal of Biosciences
Website

Abbrev

hayati

Publisher

Institut Pertanian Bogor

Subject

Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

Description

HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, ...