HAYATI Journal of Biosciences
Vol. 30 No. 1 (2023): January 2023

The Effects of Ultraviolet B on The Efficacy of Bacillus thuringiensis var. kurstaki Formulations Against Tobacco Armyworm, Spodoptera litura (Lepidoptera: Noctuidae)

Sukirno Sukirno (Entomology Laboratory, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia)
Siti Sumarmi (Entomology Laboratory, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia)
R.C. Hidayat Soesilohadi (Entomology Laboratory, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia)
Ign. Sudaryadi (Entomology Laboratory, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia)
Hari Purwanto (Entomology Laboratory, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia)
Abdulrahman Saad Aldawood (Plan Protection Department, College of Food and Agriculture Science, King Saud University, Riyadh, Saudi Arabia)



Article Info

Publish Date
19 Aug 2022

Abstract

Tobacco armyworm (Spodoptera litura Fab.) is one of the major insect pests of crops in Indonesia. The management of this pest still depends on the use of chemical insecticides. The use of bio-insecticides, such as Bacillus thuringiensis (Bt.), are known to be alternatives, but it easily degraded by sunlight. This research aimed to study the effects of UV B on Bt. pathogenicity and to explore plant-based additives as UV B protectants for Bt. against armyworm. Thirteen plant extracts were screened based on their UV spectra absorbencies using UV spectrophotometry. The extracts, namely cloves, Jicama, Celebes pepper, turmeric, and Moringa, then used for the formulations of Bt. and exposed under UV B lights for 0, 72, and 144 h. After exposure to UV B, Bt. formulations were tested for bioassay against one- day-old 1st and one day old 2nd larval instars of tobacco armyworm. The result indicated that at 72 and 144 h of UV B exposures, the Bt. added formulations were signifcantly different compared to Bt. alone. At 72 h exposure against the 2nd larval instar, the larval mortality of tobacco armyworm on the second day of observation on Bt. formulated with Celebes pepper and turmeric was 97.3 and 80%, respectively, whereas, at 144 h exposure, the mortality was 96 and 89.3%, respectively. This study concluded that Celebes pepper and turmeric extracts were the potential to be used as the Bt. protectants against UV B.

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Journal Info

Abbrev

hayati

Publisher

Subject

Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

Description

HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, ...