Jurnal Ilmu Kehutanan
Vol 16 No 2 (2022): September

Pengaruh Perbedaan Jenis Pelarut terhadap Kandungan Senyawa Kimia, Aktivitas Antioksidan, dan Kadar Sitotoksisitas dari Ekstrak Gum Acacia Decurrens Willd.

Ramadhany Ayu Purnama (Universitas Gadjah Mada)
Sigit Sunarta (Universitas Gadjah Mada)
Hilda Ismail (Universitas Gadjah Mada)



Article Info

Publish Date
12 Sep 2022

Abstract

This study aimed to determine the chemical compounds in A. decurrens gum and its bioactivity potential. Extraction was carried out in multilevel with the reflux technique using n-hexane, ethanol, and water as solvents. The chemical compounds were identified using Gas Chromatography-Mass Spectrometry (GC-MS). The amount of A. decurrens gum extract yield, total phenolic content (TPC), total flavonoid content (TFC), and total flavonols content (TVC) were then analyzed quantitatively. Furthermore, bioactivity in the form of antioxidant activity was analyzed with the 2,2-diphenyl-1-picrylhydrazil (DPPH) method, while the cytotoxicity level was assessed using the brine shrimp lethality assay (BSLT). The GC-MS analysis showed that the n-hexane-soluble fraction contained alkane, alkene, aldehyde, ester, and aromatics, while the ethanol-soluble fraction contained monosaccharide and fat group compounds. The water-soluble fraction contained only the monosaccharide group. The highest content value was obtained from the ethanol-soluble fraction, namely TPC, TFC, TVC, and antioxidant activity of 19.61±0.47 mgGA/g, 23.89±1.32 mgQE/g, 13.41±0.81 mgCE/g, and IC50: 3.842 g/mL, respectively. The cytotoxicity content obtained from each fraction sequentially was 0.47 ppm, 5.91 ppm, and 6.56 ppm for n-hexane, ethanol, and water, respectively.

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Journal Info

Abbrev

jik

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Jurnal Ilmu Kehutanan is a biannual open access journal by the Faculty of Forestry, Universitas Gadjah Mada, Indonesia, that publishes peer-reviewed scientific articles focusing on aspects of forest management, silviculture, forest conservation, and forest technology, both basic and applied. The ...