AGRIVITA, Journal of Agricultural Science
Vol 45, No 1 (2023)

Molecular Identification of Microbes from the Soil Rhizosphere of Cocoa as A Potential Biofertilizer

Nurmayulis Nurmayulis (Sultan Ageng Tirtayasa University, Serang, Banten, Indonesia)
Abdul Hasyim Sodiq (Sultan Ageng Tirtayasa University, Serang, Banten, Indonesia)
Fitria Riany Eris (Sultan Ageng Tirtayasa University, Serang, Banten, Indonesia)
Dewi Hastuti (Sultan Ageng Tirtayasa University, Serang, Banten, Indonesia)
Yus Rama Denny (Sultan Ageng Tirtayasa University, Serang, Banten, Indonesia)
Dwi Ningsih Susilowati (Research Center for Horticulture and Estate Crops, National Research and Innovation Agency (BRIN), Indonesia)



Article Info

Publish Date
27 Feb 2023

Abstract

There are three microbes that originated from the roots (Rhizosphere) of the cocoa plant (Theobroma cacao L.) as a potential biofertilizer. This research aimed to specify the ability of microbial isolates in a consortium with the addition of Biosurfactant Diethanolamide. The three microbes were observed using synergism test, molecular and population identification in the consortium. The synergism test showed the three isolates were not mutually antagonistic indicated there was no clear zone around the colony. The molecular identification showed the isolate 1 was as Bacillus substilis (99.88% hegemony), isolate 2 was as Bacillus substilis (99.75% hegemony) and isolate 3 was as Bacillus amyloliquefaciens (100% hegemony). The population consisted of  3.6 x 106 cfu/ml Azotobacter, 0.55 x 101 cfu/ml Azospirillium and 2.5 x 105 cfu/ml phosphate solubilizing bacteria. The results of the calculations on the microbial consortium with the addition of Biosurfactant Diethanolamide were 3.75 x 106 cfu/ml Azotobacter, 0.1 x 102 cfu/ml Azospirillium and 1.65 x 105 cfu/ml phosphate solubilizing bacteria.

Copyrights © 2023






Journal Info

Abbrev

AGRIVITA

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

AGRIVITA Journal of Agricultural Science is a peer-reviewed, scientific journal published by Faculty of Agriculture Universitas Brawijaya Indonesia in collaboration with Indonesian Agronomy Association (PERAGI). The aims of the journal are to publish and disseminate high quality, original research ...