Medical Sains : Jurnal Ilmiah Kefarmasian
Vol 8 No 3 (2023)

PHYTOCHEMICALS SCREENING AND ANTIOXIDANT ACTIVITY TEST OF ETHYL ACETATE FRACTION OF CHINESE BETLE LEAVES (Peperomia pellucida L.)

Naida Rahma Almira (Sekolah Tinggi Farmasi Muhammadiyah Cirebon)
Didin Ahidin (Sekolah Tinggi Farmasi Muhammadiyah Cirebon)
Iin Indawati (Sekolah Tinggi Farmasi Muhammadiyah Cirebon)



Article Info

Publish Date
23 Aug 2023

Abstract

Peperomia pellucida (Chinese betel) is considered as a plant with antioxidant activity for  inhibiting oxidation reactions. This study aims to determine the yield of secondary metabolites, total flavonoid levels, and antioxidant activity. Dried leaves of Chinese betel were macerated using 70% ethanol as solvent. The extract was partitioned until the n-hexane and ethyl acetate fractions were obtained. A phytochemical screening test was followed by determining total flavonoid levels and antioxidant activity using UV-Vis spectrophotometry. Phytochemical screening results containing an ethanolic extract of Chinese betel leaves (ECBL) showed positive results on alkaloid, flavonoid, saponin, and tannin. Meanwhile, the ethyl acetate (EAF) and n-hexane (HF) fractions showed positive results only for flavonoid presence. The total flavonoid contents of ECBL, EAF, and HF were 3.30%, 2.54%, and 2.03%, respectively. The IC50 values of  ECBL, EAF, and HF were 7.51 ppm, 8.02 ppm, and 8.04 ppm, respectively. The highest flavonoid content was found in ECBL. Antioxidant activity test of Peperomia pellucida ethanolic extract, ethyl acetate fraction, and n-hexane fraction showed potent antioxidant activity.  Keywords: Antioxidant, Peperomia pellucida , DPPH

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Journal Info

Abbrev

iojs

Publisher

Subject

Medicine & Pharmacology

Description

The Medical Sains journal is a scientific publication media published by the Muhammadiyah Cirebon College of Pharmacy which is published 4 (four) times in 1 (one) year, namely January-March, April-June, July-September and October-December. The journal contains research in the field of pharmacy ...