Jurnal Natural Scientiae (JNS)
Vol 1, No 1 (2021): November 2021

Uji Sitotoksik Ekstrak Alkaloid Daun Belimbing Wuluh (Averrhoa bilimbi L.) terhadap Larva Udang (Artemia salina)

Mariani Mariani (Universitas Lambung Mangkurat)
Kholifatu Rosyidah (Universitas Lambung Mangkurat)
Kamilia Mustikasari (Universitas Lambung Mangkurat)

Article Info

Publish Date
10 Dec 2021

Abstract

Research on cytotoxic test of alkaloids extract of belimbing wuluh leaves has been conducted. The objective of this research are to determine the yield, toxicity, TLC chromatogram pattern, and compounds in alkaloids extract of belimbing wuluh leaves with GC-MS. Alkaloid extraction process uses acid base method, the phytochemical tests was carried out with reagents Dragendorff, Wagner and Meyer. Toxicity test of alkaloids extract of belimbing wuluh leaves with BSLT method on A. salina larvae to determine LC50 value. Alkaloids extract of belimbing wuluh leaves were analyzed by TLC and GC-MS. The yield of alkaloids extract of belimbing wuluh leaves obtained was 10, 13 %. The results of phytochemical tests were carried out which showed positive containing alkaloids. LC50 value of the alkaloids extract of belimbing wuluh leaves indicated that is very toxic. The LC50value obtained is 9.170 ppm. Analysis using TLC with eluent of n-hexane: ethyl acetate (8:2) showed 5 stains. GC-MS analysis showed are alkaloid compound with an odd molecular weight.

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Journal Info
Jurnal Natural Scientiae (JNS)
Website

Abbrev

jns

Publisher

Universitas Lambung Mangkurat

Subject

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Energy Immunology & microbiology Materials Science & Nanotechnology Physics

Description

Jurnal Natural Scientiae (JNS), ISSN:2809-9796 (online) adalah sebuah jurnal blind peer review yang didedikasikan untuk publikasi hasil penelitian yang berkualitas dalam bidang IPA namun tak terbatas secara implisit. Semua publikasi di jurnal Natural Scientiae bersifat akses terbuka yang ...