Journal of Pharmaceutical and Sciences.
JPS Volume 6 Nomor 4 (2023)

Identifikasi DNA babi sebagai metode validasi untuk autentikasi halal menggunakan polymerase chain reaction

Mariyani Mariyani (Sekolah Tinggi Ilmu Farmasi Pelita Mas Palu, Kota Palu, Sulawesi Tengah, Indonesia)
Ni nyoman Yuvita Yani (Sekolah Tinggi Ilmu Farmasi Pelita Mas Palu, Kota Palu, Sulawesi Tengah, Indonesia)
Ikhsan Hi. Amir Sene (Akademi Farmasi Tadulako Farma Palu, Kota Palu, Sulawesi Tengah, Indonesia)

Article Info

Publish Date
19 Nov 2023

Abstract

To protect consumers from fake halal labelling on food products, cosmetics and medicines, a method is needed to guarantee a product's Halal. Pork is one type that is often used to mix with beef because the two types of meat have physical similarities if not carefully considered. DNA-based analysis, often used for halal authentication, is the real-time PCR method, so this study aims to prove that conventional PCR methods can detect DNA at the same concentration. This study uses two parameters: the specificity test carried out using pig DNA samples and cow and chicken DNA as a comparison. The second parameter is the detection limit test on absolute DNA carried out at 4 concentrations, namely 50, 5, 0.5 and 0.05 ng/µL, while the relative detection limit test (pork-cow mixture) with variations in pork concentration, namely 100%, 5%, 3%, 1%, and 0.5%. The analysis showed that the primers were specific to pig DNA with an absolute detection limit of 0.05 ng/µL and a relative detection limit of 0.5%. This PCR method meets the validation requirements for identifying target species so that it can be used for halal authentication of various products.

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Journal Info
Journal of Pharmaceutical and Sciences.
Website

Abbrev

jps

Publisher

Universitas Tjut Nyak Dhien

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health

Description

Journal of Pharmaceutical and Sciences (JPS) with e-ISSN: 2656-3088 is a scholarly peer-reviewed open access journal related to Pharmaceutics, Pharmacology, biotechnology, Pharmacognosy & Phytochemistry, Pharmaceutical Chemistry, Pharmaceutical Biotechnology, Pharmacy practice, Pharmacogenomics, ...