Jurnal Natural
Volume 22 Number 3, October 2022

Comparison of two DNA extraction methods for dry Leaf Dipterocarpaceae

ESSY HARNELLY (Department of Biology, Faculty of Mathematics and Science, University of Syiah Kuala, Banda Aceh, Indonesia)
NITA TAUHIDA (Department of Biology, Faculty of Mathematics and Science, University of Syiah Kuala, Banda Aceh, Indonesia)
ISKANDAR ZULKARNAIN SIREGAR (Faculty of Forestry, IPB University, Bogor, Indonesia)

Article Info

Publish Date
31 Oct 2022

Abstract

DNA extraction is the first step in obtaining high-concentration and high-purity DNA that can use in the subsequent steps. Because the leaf structure is challenging and contains several secondary metabolites that can affect DNA extraction results, DNA extraction on dry leaves of Dipterocarpaceae is considered problematic. This research aims to find a suitable method for extracting DNA from the preserved Dipterocarpaceae leaves. Preserved leaves of Dipterocarpaceae in silica gel will dry up, making them tough to destroy. Perform DNA extraction is generally using CTAB buffers or extraction kits. According to the results of this study, extraction with modified CTAB buffer result in DNA with higher concentration and purity values than DNA extracted with the kit. DNA isolated from the modified CTAB can be used as a template in the PCR process to amplify the matK gene.

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Journal Info
Jurnal Natural
Website

Abbrev

natural

Publisher

Universitas Syiah Kuala

Subject

Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Energy Immunology & microbiology Neuroscience Physics

Description

Jurnal Natural (JN) aims to publish original research results and reviews on sciences and mathematics. Jurnal Natural (JN) encompasses a broad range of research topics in chemistry, pharmacy, biology, physics, mathematics, statistics, informatic and ...