UNEJ e-Proceeding
Indonesian Protein Society (IPS), International Seminar and Workshop 2014

Optimization pH of Enzymatic Hydrolysis of Endo-1,4-β-Xylanase for Xylooligosaccharides Production

Ratnadewi, Anak Agung Istri ( Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Jember. Center for Development of Advanced Sciences and Technology, Universitas Jember)
Kurniawan, Andika Ade ( Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Jember. Center for Development of Advanced Sciences and Technology, Universitas Jember)
Handayani, Wuryanti ( Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Jember. Center for Development of Advanced Sciences and Technology, Universitas Jember)

Article Info

Publish Date
24 Feb 2016

Abstract

Xylooligosaccharides (XOS) with polymerisation degree between 2 till 10 monomer  have prebiotic effect for better digestion system. In this research, production of XOS was performed by enzymatic hydrolysis of xylan with endo-1,4-β-xylanases enzyme. Endo 1,4-β-xylanases enzyme was aqcuired from Bacillus sp. isolated from termite’s abdominal.Meanwhile oat xylan was used as substrate. Optimal condition of enzymatic hydrolysis was evaluated at pH: 4, 5, 6, and 7 with incubation time from 5 to 20 h.pH 5 was optimum pH to produce XOS from 0.8 % xylan oat at 40 oC.The hydrolysis product purified and analyzed by thin layer chromatographyyielding spot of xylobiose, xylotriose, xilotetraose and xilopentaose. Further analysis by HPLC indicated dominant xilopentaosa X5 (3522 ppm) among  the other XOS   X2 (14 ppm), X3 (43 ppm) and X4 (15 ppm). Keywords: Xylooligosaccharides, endo-1,4-β- endoxylanase

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UNEJ e-Proceeding
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Universitas Jember

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Education

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