Majalah Obat Tradisional
Vol 22, No 2 (2017)

Antituberculosis Activity of Brotowali (Tinospora crispa) Extract and Fractions against Mycobacterium tuberculosis using Microplate Alamar Blue Assay Method

Retno Wahyuningrum (Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Indonesia Faculty of Pharmacy Universitas Muhammadiyah Purwokerto, Indonesia)
Ritmaleni Ritmaleni (Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta)
Tatang Irianti (Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta)
Subagus Wahyuono (Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta)
Takushi Kaneko (Tuberculosis Alliance, New York)
Titik Nuryastuti (Faculty of Medicine Universitas Gadjah Mada, Yogyakarta)

Article Info

Publish Date
31 Aug 2017


Tuberculosis (TB), in which caused by pathogenic bacteria, Mycobacterium tuberculosis, has become the major causes of death among all of infectious diseases. The increasing incidence of multidrug-resistant tuberculosis (MDR-TB) and extensively drug-resistant tuberculosis (XDR-TB) has created a need to discover a new antituberculosis drug candidate. The aim of this study was to screen extract and fractions of Tinospora crispa for activity against Mycobacterium tuberculosis H37Rv using the Microplate Alamar Blue Assay (MABA) method. T. crispa extract was prepared by maceration in ethanol (96%) and antituberculosis activity was carried out using MABA method. The result of this study showed that ethanolic extract of T. crispa exhibit antituberculosis activity with minimum inhibition concentration of 12.5 mg/ml.

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Journal Info





Health Professions Medicine & Pharmacology Public Health


raditional Medicine Journal (Majalah Obat Tradisional), or Trad. Med. J. (ISSN 1410-5918 (print) and ISSN 2406-9086 (online)), is an international scientific journal published by Faculty of Pharmacy, Universitas Gadjah Mada, three times annually. Collaborating with Indonesian Pharmacist Association, ...