Indonesian Journal of Biotechnology
Vol 13, No 1 (2008)

Identification of Thymocyte Subset by Multicolor Flow Cytometry ED LSR II FACSDriva - FlowJo Software Analysis

H. Harapan (Unknown)
W. Wienands (Unknown)
I. Ichsan (Unknown)
Ana Indrayati (Unknown)

Article Info

Publish Date
01 Jun 2008

Abstract

In their development, thymocytes express different cell surface molecules that important for identification of thymocyte subset. It’s not easy to detect this cell surface molecules to determine the thymocyte subpopulation for research. Here we used multicolor flow cytometry ED LSR II FACSDriva - FlowJo software to identify of thymocyte subset from thymocyte sample solution using several antibodies such as mouse anti rat CD2-FITC, mouse anti rat CD45RC-PE, mouse anti rat CD4-APC, mouse anti rat CD8á-PerCP, mouse anti rat CD3-Biotin + PE-Cy7 or APC-Cy7. We determined double negative and single positive thymocyt subset (CD4 or CD8), found that the double negative thymocyte subset express CD2 and CD45RC. It was useful to determine the thymocyte subset using multicolor flow sitometry ED LSR II FACSDriva - FlowJo software.

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Journal Info
Indonesian Journal of Biotechnology
Website

Abbrev

ijbiotech

Publisher

Universitas Gadjah Mada

Subject

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology

Description

The Indonesian Journal of Biotechnology (IJBiotech) is an open access, peer-reviewed, multidisciplinary journal dedicated to the publication of novel research in all aspects of biotechnology, with particular attention paid to the exploration and development of natural products derived from ...