ALCHEMY Jurnal Penelitian Kimia
Vol 11, No 1 (2015): Maret

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD VALIDATION OF α-MANGOSTIN ASSAY IN MANGOSTEEN (Garcinia mangostana L.) FRUIT RIND EXTRACT FORMULATED IN ORAL SOLUTION

Liliek Nurhidayati (Fakultas Farmasi, Universitas Pancasila, Jakarta Selatan)
Siti Sofiah (Fakultas Farmasi, Universitas Pancasila, Jakarta Selatan)
Ros Sumarny (Fakultas Farmasi, Universitas Pancasila, Jakarta Selatan)
Kevin Caesar (Fakultas Farmasi, Universitas Pancasila, Jakarta Selatan)

Article Info

Publish Date
27 Sep 2016

Abstract

Mangosteen fruit rind extract contain a lot of antioxidants. α-Mangostin is a component in mangosteen fruit rind that has highest antioxidant effect. The oral solution containing mangosteen fruit rind extract is required an assay method for quality assessment. Determination of a very low concentration of analyte in sample with very complex matrix, such as α-mangostin in oral solution, needs a selective and sensitive method, such as high performance liquid chromatography (HPLC). In this study, α-mangostin assay was performed by reverse phase HPLC system using octadecylsilane (C18) as stationary phase,  methanol-water (90:10) as mobile phase, the flow rate is 1.0 mL/min, and the UV detector at 316 nm. The retention time of α-mangostin was 9.622 minutes. Peak of α-mangostin was well separated with resolution of 1.725. Linearity was in the range of 1.67-5.01 ppm with correlation coefficient of 0.9986. The relative standard deviation (RSD) was 1.30 %, the recovery was in the range of 95.80-100.76 

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Journal Info
ALCHEMY Jurnal Penelitian Kimia
Website

Abbrev

alchemy

Publisher

Universitas Sebelas Maret

Subject

Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science Materials Science & Nanotechnology

Description

ALCHEMY Jurnal Penelitian Kimia is a chemistry journal published by Sebelas Maret University, Surakarta. ALCHEMY Jurnal Penelitian Kimia publishes original research articles or review articles in organic chemistry, inorganic chemistry, analytical chemistry, physical chemistry, biochemistry, and ...