Warta IHP (Warta Industri Hasil Pertanian)
Vol 28, No 02 (2011)

(Method for HPLC Analysis of Active Compounds in Ginger (Zingiber officinale)

Yus Maria Novelina.S (Balai Besar Industri Agro)
M Maman Rohaman (Balai Besar Industri Agro)
Hendra Wijaya (Balai Besar Industri Agro)



Article Info

Publish Date
07 Jul 2017

Abstract

The studies Ginger (zingiber officinale) is one of herb plant which contains various of active compounds,which is proved has function hasĀ  to human health .in indonesia , there are 3 main ginger variaties,those are :giant gingger (zingiber officinale var .officinale),emprit ginger (Zingiber officinale var. amarum ), and red ginger (Zingiber officinale var.rubrum) .Each ofthem has different characteristic and function .the problems which often occurs are ginger counterfeting,often mixed with other gingers.this counterfeiting difficult to be detected with conventional test method. therefore needed study about characteristic of each ginger with HPLC so counterfeiting able to be traced .Asensitive and accurate high-performance liquid chromatography (HPLC) method has been developed to determine the quantity of active componen in the methanolic extract of Zingiber officinale Bioactive component was separated on persuit C18 Column (250 mm x4.6 mm,particle size:5 um)with gradient system of water and acetonitrile as mobile phase. the calibration plot was linear in the range of 0.10-150 mg/L .of 6-,8-,10-gingerol and the correlation coefficient of o.9998 was indicative of good linear dependence of peak area on concentration .the limit of detection (LOD) OF 6-gingerol ,8-ginggerol ,6-shogaol andĀ  6-shogaol and the correlation coefficient were 2,34 1,06; 1,25, and 0,67 mg/l and limit of detection (LOD)were 7,81 :3,53 :3,53; 4,18 and 2,29 mg/lrespectively.the developed HPLC method was found selective,precise and accurate,and can be used for routine analysis of gingers in the quality control laboratories .

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Journal Info

Abbrev

ihp

Publisher

Subject

Agriculture, Biological Sciences & Forestry Industrial & Manufacturing Engineering

Description

Warta IHP (Industri Hasil Pertanian) is a Scientific Journal which is sourced from research papers, new theoretical/interpretive findings, and critical studies or reviews (by invitation) in the agro-based industry scope that cover any discipline such as: food science and technology, agricultural ...