Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
Vol 23, No 1 (2006)

Pengaruh Elisitasi dengan Verticillium dahliae Kleb dan Rhizoctonia Solani Kuhn terhadap kandungan Gosipol dalam kalus Gossypium hirsutum L pada beberapa tingkat Subkultur

Suci Rahayu (Unknown)
Rizkita Rachmi Esyanti (Unknown)
Arbayah H Siregar (Unknown)



Article Info

Publish Date
10 Jan 2006

Abstract

An experiment on gossypol content hasbeen conducted on callus derived from Gossypium hirsutum L. subcultures 5, 6, and 7 after elicitation with Verticillium dahliae Kleb and Rhizoctonia solani Kuhn. Callus was cultured on solid Linsmaier and Skoog  (LS)  medium  supplemented  with 10 –5 M NAA and 10 –6 M 2,4-D, and subcultured for 5, 6, and 7 times. Callus was then elicited with 40  μg dry weight /ml homogenate of fungi derived from V. dahliae and R. solani, and harvested 0, 2, 4, and 6 days after elicitation. The gossypol was analyzed qualitatively and quantitatively using High Performance Liquid Chromatography (HPLC). The maximum gossypol content elicited with R. solani was obtained on callus subculture 5, which was harvested on 4 days after elicitation, i.e. 177,995 ±  0,248 μg/g dry weight,  whilst that with V. dahliae  was obtained on subculture 5, harvested four days after elicitation, i.e.108,021± 0,507 μg/g dry weight. The gossypol content of control callus on subculture 5, 6, and 7 was 37,885 ± 0,779 μg/g dry weight  23,170 ±  0,003 μg/g dry weight, 12,284 ± 0,221 μg/g dry weight, respectively. The subculture level, elicitor type, and harvesting time gave significant effect on gossypol content of G. hirsutum callus culture.

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Journal Info

Abbrev

biosfera

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

Description

Majalah Ilmiah Biologi Biosfera : A Scientific Journal merupakan peer reviewed jurnal yang diterbitkan oleh Fakultas Biologi Universitas Jenderal Soedirman. Media ini mewadahi hasil-hasil penelitian di bidang biologi tropika yang terbit tiga kali setahun (Januari, Mei, ...