Jurnal Sains Natural
Vol. 4 No. 2 (2014): Sains Natural

ANTIBAKTERI EKSTRAK KULIT BUAH MANGGIS (Garcinia mangostana L.)

Srikandi Srikandi (Program Studi Biologi FMIPA, Universitas Nusa Bangsa Jl. K.H. Sholeh Iskandar Km. 4, Cimanggu, Tanah Sareal Bogor 16166)
I.G.A. Manik Widhyastini (Program Studi Biologi FMIPA, Universitas Nusa Bangsa Jl. K.H. Sholeh Iskandar Km. 4, Cimanggu, Tanah Sareal Bogor 16166)



Article Info

Publish Date
03 Dec 2017

Abstract

Antibacterial Extract of Mangosteen (Garcinia mangostana L.)Fruit Shell           The skin of the mangosteen fruit is extracted with n-hexane and ethyl acetate to determine the Minimum Inhibitory Concentration (MIC) against Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27 853. Results showed that n-hexane extract gave inhibition zone larger than the ethyl acetate extract on all concentrations . Extract n-hexane has a value of MIC against S. aureus ATCC bacterial test 25923 62.5 mg / ml while the ethyl acetate extract of 125 mg / ml . N- hexane extracts had MIC values of the test bacteria P.aeuroginosa ATCC 27 853 was 125 mg / ml , and while the ethyl acetate extract had a MIC value of 500 mg / ml . Treatment of solvent, concentration and interaction between the solvent and concentration significantly affected the test bacteria ATCC 25923 S. aureus at the level of 5 %, the highest interaction N-Hexane solvent with a concentration of 15,625 mg / ml and was not significantly different interactions with the concentration of 31.25 mg/ml and 125 mg/ml. Treatment solvent and concentration significantly while the interaction between the solvent and the concentration has no effect on the test bacteria P.aeuroginosa ATCC 27 853 at 5% level .Keywords: Garcinia mangostana L., Staphylococcus aureus and Pseudomonas aeruginosa ABSTRAK           Kulit buah manggis diekstrak dengan n-heksan dan etil asetat   untuk mengetahui Konsentrasi Hambat Minimum (KHTM) terhadap bakteri Staphylococcus aureus ATCC 25923 dan Pseudomonas aeruginosa ATCC 27853. Hasil penelitian menunjukkan bahwa ekstrak n-heksana  memberikan zona hambatan lebih besar dibandingkan dengan ekstrak etil asetat pada semua konsentrasi. Ekstrak n-heksana  memiliki nilai kadar hambat minimal (KHM) terhadap bakteri uji S. aureus ATCC 25923  62,5 mg/ml sedangkan ekstrak etil asetat 125 mg/ml.   Ekstrak n-heksana memiliki nilai KHM   terhadap bakteri uji P.aeuroginosa  ATCC 27853 adalah 125 mg/ml dan  sedangkan ekstrak etil asetat memiliki nilai KHM  500 mg/ml. Perlakuan pelarut, konsentrasi dan interaksi antara pelarut dan konsentrasi  berpengaruh nyata terhadap bakteri uji S. aureus ATCC 25923 pada taraf 5%, interaksi tertinggi yaitu pelarut N-Heksan dengan konsentrasi 15,625mg/ml dan interaksi ini tidak berbeda nyata dengan konsentrasi 31,25 mg/ml dan 125 mg/ml. Perlakuan pelarut dan konsentrasi  berpengaruh nyata sedangkan interaksi antara pelarut dan konsentrasi  tidak berpengaruh terhadap bakteri uji P.aeuroginosa  ATCC 27853 pada taraf 5%. Kata kunci:  Garcinia mangostana L., Staphylococcus aureus dan Pseudomonas aeruginosa

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Journal Info

Abbrev

JSN

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry

Description

Jurnal Sains Natural is a peer-reviewed, open access journal that publishes original research articles, review articles, as well as short communication with the objectives to explore the knowledge about natural sciences. This journal incorporates not only all branches of chemistry and biology, but ...