Indonesian Aquaculture Journal
Vol 8, No 1 (2013): (June 2013)

PCR-DGGE METHOD FOR MICROFLORA ALTERATION IN SHRIMP DIGESTIVE ORGAN FOLLOWING Lactobacillus ADMINISTRATION

Nunak Nafiqoh (Research and Development Institute for Freshwater Aquaculture, Jl. Sempur No. 1, Bogor 16151, Indonesia)
Poh-Shing Chang (Research and Development Institute for Freshwater Aquaculture, Jl. Sempur No. 1, Bogor 16151, Indonesia)
Yu-Chi Wang (Research and Development Institute for Freshwater Aquaculture, Jl. Sempur No. 1, Bogor 16151, Indonesia)



Article Info

Publish Date
30 Jun 2013

Abstract

Recently, bacteria as probiotic usage in aquaculture are deeply investigated. Probiotic application in aquaculture leads to alteration in bacteria community within environment and inside digestive system of the host. However, the evidence of treated bacteria presence is very limit. This study was aimed to reveal the effectiveness of PCR-DGGE method to bring some evidence of the applied probiotic existence within aquatic organism. Two species of Lactobacillus were applied in this experiment. Litopenaeus vannamei was used as host for applied bacteria, L. vannamei was reared using natural sea water in the 45 cm x 15 cm x 25 cm aerated glass tank. Twice daily of enriched Artemia were gived as nutrition during experiment. The result showed that Lactobacillus represent higher in treatment group compare with control after 20 days treatment. In the other hand there was no different of bacteria number between two treated Lactobacillus. PCR-DGGE is a rapid and reliable method for bacteria detection within aquatic organism.

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Journal Info

Abbrev

iaj

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

Indonesian Aquaculture Journal is a peer-reviewed and open access journal based in Indonesia that globally/internationally accepts and publishes scientific articles in the field of aquaculture. The journal is hosted and managed by the Center for Fisheries Research, Indonesian Ministry of Marine ...