Jurnal Riset Akuakultur
Vol 4, No 1 (2009): (April 2009)

KARAKTERISTIK GENETIK POPULASI TIRAM MUTIARA (Pinctada margaritifera) TERKAIT DENGAN DISTRIBUSI GEOGRAFISNYA DI PERAIRAN INDONESIA

Rini Susilowati (Balai Besar Riset Bioteknologi dan Pengolahan Produk Kelautan dan Perikanan, Jakarta)
Komar Sumantadinata (Departemen Budidaya Perairan-FPIK, Institut Pertanian Bogor, Bogor)
Dinar Tri Soelistyowati (Departemen Budidaya Perairan-FPIK, Institut Pertanian Bogor, Bogor)
Achmad Sudradjat (Pusat Riset Perikanan Budidaya, Jakarta)



Article Info

Publish Date
30 Apr 2009

Abstract

Tujuan penelitian ini untuk memetakan keragaman genetik lima populasi tiram mutiara di Indonesia (Sumbawa, Bali Utara, Selat Sunda, Belitung, Sulawesi Selatan) dengan teknik mtDNA RFLP daerah amplifikasi Cytochrome Oxydase I (COI) dan hubungan kekerabatannya. Lima puluh tiram mutiara (Pinctada margaritifera) yang dianalisis menghasilkan DNA teramplifikasi sebesar 750 pb pada daerah COI mtDNA dengan teknik RFLP. Delapan belas komposit haplotipe terdeteksi dengan menggunakan tiga enzim restriksi: FokI, HaeIII, dan NlaIV. Diversitas haplotip rata-rata sebesar 0,255±0,093. Lima populasi tiram mutiara menghasilkan tiga kelompok dengan jarak genetik terendah adalah populasi Sumbawa dan Bali Utara (0,017) dan terjauh adalah populasi Sulawesi Selatan (0,142). Populasi Sulawesi Selatan merupakan populasi unik berdasarkan distribusi haplotipe BBCAA (60%) dengan nilai keragaman genetik terendah (0,105) dibandingkan dengan populasi lainnya (0,177-0,328).The objectives of this study were to map the genetic diversity of five populations of pearl oyster in Indonesian waters using restriction fragment length polymorphism analysis of DNA COI gene and their genetic relationships. A total of 50 individual of pearl oysters (Pinctada margaritifera) were analyzed for genetic variations within a 750-base pair region of the mitochondrial DNA COI gene using restriction fragment length polymorphism analysis. 18 composite haplotypes were detected following three digestions of endonuclease: FokI, HaeIII, and NlaIV. Five populations of pearl oysters formed three groups where the lowest values of Nei’s genetic distance were among Sumbawa and North Bali populations (0.017) and highest were among the South Sulawesi populations (0.142). The South Sulawesi populations possess uniqueness based on the haplotipe distribution of BBCAA (60%) with the lowest values of genetic diversities (0.105) compared to other populations (0.177--0.328).

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Journal Info

Abbrev

jra

Publisher

Subject

Agriculture, Biological Sciences & Forestry Environmental Science

Description

Jurnal Riset Akuakultur as source of information in the form of the results of research and scientific review (review) in the field of various aquaculture disciplines include genetics and reproduction, biotechnology, nutrition and feed, fish health and the environment, and land resources in ...