Jurnal Rekayasa Lingkungan
Vol 5, No 1: Jurnal Rekayasa Lingkungan

PEMANFAATAN KULIT BUAH PISANG NANGKA SEBAGAI SUBSTRAT FERMENTASI PADAT PADA PRODUKSI XILANASE

dkk, Trismilah (Unknown)



Article Info

Publish Date
04 Apr 2018

Abstract

Jackfruit skin bananas (Musa sp.) one of the agricultural waste that is rich nutrients for the growth of microorganisms, can be used as a substrate of xilan for the production xilanase. The research aims to know the optimum conditionsof solid fermentation for production xilanase Bacillus licheniformis I-5 using a jackfruit skin banana as substrate. Optimization includes the incubation time for 72 hours with the interval measurement activities every six hours, the moisture content variation on the 1: 1.0 (55%); 1: 1.5 (65%); 1: 2.0 (70%); 1 : 2.5 (74%) (w / v), incubation temperature variation in the 40 °, 45 °, 50 °, 55 °, 60 ° C and the addition of carbon and nitrogen source on the concentration of 1, 2,3, 4, and 5% (w / w). Fermentation carried out in erlenmeyer 250 ml, containing 10 g cod banana jackfruit, 0.4 K2HPO4, and 0.2 MgSO4 (g / l). Results of research shows that the optimum activity xilanase to 48 hours of fermentation,moisture content 1: 1.5 (65%) and incubation temperature 50 ° C with the activity of 0410 ± 0102 U / ml. The addition of glucose and prevent xilosa activities to be 0032 ± 0007 U / ml and 0053 ± 0025 U / ml concentration in 5%. Pepton addition of 4% increased the activity is not signifi cant to control the 0487 ± 0073 U / ml.

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