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INDONESIA
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
Published by Pusat Litbang Daya Saing Produk dan Bioteknologi Kelautan dan Perikanan
ISSN : 20895690     EISSN : 24069272     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
Squalen publishes original and innovative research to provide readers with the latest research, knowledge, emerging technologies, postharvest, processing and preservation, food safety and environment, biotechnology and bio-discovery of marine and fisheries. The key focus of the research should be on marine and fishery and the manuscript should include a fundamental discussion of the research findings and their significance. Manuscripts that simply report data without providing a detailed interpretation of the results are unlikely to be accepted for publication in the journal.
Arjuna Subject : -
Articles 8 Documents
 1 
Search results for , issue "Vol 13, No 2 (2018): August 2018" : 8 Documents clear
Risk Profile and Semi Quantitative Risk Probability of Aflatoxin B1 from Aspergillus flavus in a Dried Salted Fish in Several Regions of Java Hidayah, Izhamil; Hermana, Irma; Kusmarwati, Arifah
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 2 (2018): August 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i2.356

Abstract

This study presents a semi-quantitative risk analysis, which determines the probability of aflatoxin B1 exposure from Aspergillus flavus in dried salted fish from the results of research conducted by Indriati about  the prevalence of aflatoxin  B1 in commercial dried fish from some regions of Java. Samples were randomly collected from retailers in Java, such as Banten, DKI Jakarta, West Java, Central Java and East Java, to obtain an approximate level of aflatoxin B1 exposure into Indonesian consumers. The occurrence of the probability of aflatoxin B1 risk from Aspergillus flavus was calculated by statistical, probability approach in @risk version 7.0 software with Monte Carlo simulation. The results of this study showed that the consumption of salted fish was about 3.7 g/capita/day. Hence  there are risks of 7.74 cfu/g A. flavus exposure and 0.7291 ppb aflatoxin B1 exposure in 1 g of a salted fish taken from sampling locations. However this value is still categorized as low risk level.
Front Cover Squalen Bulletin Vol. 13 No. 2 Tahun 2018 Squalen, Bulletin
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 2 (2018): August 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i2.357

Abstract

Back Cover Squalen Bulletin Vol. 13 No. 2 Tahun 2018 Bulletin Squalen
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 2 (2018): August 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i2.358

Abstract

Angiotensin Converting Enzyme (ACE) Inhibitory Activity of Crude and Fractionated Snakehead Fish (Channa striata) Fillet Extract Setyani Budiari; Ekowati Chasanah; Maggy Thenawidjaja Suhartono; Nurheni Sri Palupi
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 2 (2018): August 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i2.345

Abstract

The existence of endogenous bioactive protein or peptide with angiotensin-converting enzyme (ACE) inhibitory activity in snakehead fish fillet is promising to be investigated. The purposes of this research were to extract ACE inhibitory endogenous protein or peptide from snakehead fish fillet and to fractionate the active compounds using ultrafiltration. The extraction employed two solvents, i.e. aquadest and 50% ethanol. Fractionation was conducted using ultrafiltration membranes of 10,000; 5,000 and 3,000 Molecular Weight Cut Off  (MWCO) to separate the protein or peptide into the sizes of 10 kDa, 5-10 kDa, 3 -5 kDa and 3 kDa. The parameters observed were protein and peptide content, ACE inhibitory activity (in vitro) and also protein and peptide profiles. The result revealed that the snakehead fish fillet contained ACE inhibitory endogenous bioactive protein or peptide. The 50% ethanol was more effective in extracting peptide of 10 kDa than the aquadest. Yet, the aquadest was better in extracting higher molecular weight protein of 10 kDa than the 50% ethanol. The fraction of 3 kDa by aquadest had the highest ACE inhibitor activity per g protein (7.85% inhibition of ACE per g protein). Thus, the fraction of 3 kDa aquadest is the most promising option for further research and development of natural anti-hypertension compound. From the result, snakehead fish fillet was potential to be utilized as a functional food as well as functional ingredient to fight hypertension.
Physicochemical Characteristics of Sodium Alginate Extracted from Turbinaria sp. and Sargassum sp. Rinta Kusumawati; Jamal Basmal; Bagus Sediadi Bandol Utomo
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 2 (2018): August 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i2.297

Abstract

Characterization of sodium alginate extracted from two species of brown seaweeds (Turbinaria sp. and Sargassum sp.) harvested from Binuangeun Beach, Banten, has been conducted. The aim of the study was to evaluate physico-chemical characteristics (moisture, whiteness, viscosity, and functional groups) of sodium alginates extracted from Turbinaria sp. and Sargassum sp. Extraction was conducted in acid solution with the following steps: washing, acid extraction, bleaching, alginic acid conversion, sodium alginate conversion, dehydration, and drying. Each extraction was conducted in duplicate using approximately 1 kg of the dry raw materials (Turbinaria sp. and Sargassum sp.). Results of the analysis showed that the yield of sodium alginate powder extracted from Sargassum sp. was 24.56+0.56% (w/w) with moisture content of 12.69+1.24%; whiteness degree of 43.80+1.71%; and viscosity of 143.43+3.25 cPs, while the yield of sodium alginate powder extracted from Turbinaria sp. was 22.69+2.12% (w/w) with moisture content of 14.77+2.55%; whiteness degree of 23.77+0.68%; and viscosity of 133.67­+4.04 cPs. Meanwhile, the commercial sodium alginate was identified to have moisture content of 16.07+0.09%, whiteness degree of 29.37+0.55% and viscosity of 102.67+4.04 cPs. This indicates that physicochemical characteristics of sodium alginate extracted from Sargassum sp. is better than those extracted from Turbinaria sp. and commercial alginate since it had lower moisture content as well as higher whiteness degree and viscosity.
Potency of Actinomycetes from Deepsea Sediment of Makassar Strait for Producing Antimicrobial Substances Ariani Hatmanti; Puspita Lisdiyanti; Jaka Widada; Subagus Wahyuono
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 2 (2018): August 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i2.351

Abstract

A study on isolation of actinomycetes from sediments of Makassar Strait have been conducted with regard to research project called Widya Nusantara Exploration (EWIN) in May-June 2013 and November 2014. The objectives of this research were to screen antimicrobial activity of 36 actinomycetes from sediments of Makassar Strait, characterized the potential isolates, and determined the metabolites produced by the selected isolate. The antimicrobial screening was conducted using agar diffusion method, while characterization of the best five of actinomycetes were using APIZYM Kit, Scanning Electron Microscope, and FTIR. Five isolates retrieved from this research had ability to inhibit the growth of four microbial testing: Escherichia coli, Bacillus subtilis, Staphyllococcus aureus and Candida albicans. The highest capability was shown by the MACMK-43 isolate that had 16S rRNA gene sequence similarity of 97.85% to Streptomyces violacens. The result shows that, the active fraction contained of 4-amino-5-cyano-6-(4’methoxyphenyl)-1-methyl-2.3-dihydropyrrolo [2,3-B] pyridine, which is commercially used for bactericide and antihistamine.
Preface Squalen Bulletin Vol. 13 No. 2 Tahun 2018 Squalen Bulletin
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 2 (2018): August 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i2.359

Abstract

Probabilistic Health Risk Assessment Due to Natural Formaldehyde Intake Through Opah Fish (Lampris Guttatus) Consumption in Indonesia Ajeng Kurniasari Putri; Umi Anissah; Farida Ariyani; Singgih Wibowo
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 2 (2018): August 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i2.354

Abstract

Opah fish (Lampris guttatus) is one of the bycatch products of Tuna fish catch. The fish currently has become one of the major exported commodities of Indonesia. However, due to its natural deterioration, the fish contained high formaldehyde up to 200 ppm which can be one of health risk when consumed. The aim of this study is to obtain probabilistic health risk assessment data due to consumption of opah fish that contaminated with natural formaldehyde. The study was initiated with analyzing the formaldehyde content of the opah fish (Lampris guttatus) stored in freezing temperature for less than three months. To evaluate the population risk associated with consumption of formaldehyde-contaminated fish, a probabilistic exposure assessment was conducted in several categories based on individual characters who are likely to consume opah fish. Exposures are grouped by sex (male and female) and age (children and adults). To identify the best fit distribution in the probabilistic exposure analysis, fish consumption, body weight and natural occurrence of formaldehyde concentration in Opah fish were fitted in the @Risk®7.0 for Microsoft Excel 2010. Risk exposure analysis was conducted based on the formaldehyde content in Opah fish (real concentration) and two other scenarios of  2 and 4 times of the Opah fish real formaldehyde content. The result showed that Opah fish caught in Indonesian waters and stored in freezing temperature for less than three months, due to deterioration process, could naturally produce formaldehyde ranged from 4,62 ± 0,00 mg/kg to 58,10 ± 0,46 mg/kg. Consequently, based on the probabilistic exposure assessment, female children in Jakarta and Surabaya were considered as in health risk problems. Extremely, the further simulations using two and four times of formaldehyde concentration exposure revealed the health risk  for both gender and all age groups  of Jakarta and Surabaya residents. Therefore, the stakeholders i.e. government and policy makers should take some priorities in formulating a proper risk management strategy on the basis of knowledge of endogenous formaldehyde present in Opah fish as well as risk management strategies for the fish consumer in Indonesia.

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