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INDONESIA
Indonesian Aquaculture Journal
ISSN : 02150883     EISSN : 25026577     DOI : -
Indonesian Aquaculture Journal is a peer-reviewed and open access journal based in Indonesia that globally/internationally accepts and publishes scientific articles in the field of aquaculture. The journal is hosted and managed by the Center for Fisheries Research, Indonesian Ministry of Marine Affairs and Fisheries and serving as a scientific platform to share research information in and contribute to the development of various disciplines of aquaculture including genetics, reproduction, nutrition and feed, fish health and diseases, engineering, and environmental assessment.
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Articles 10 Documents
Search results for , issue "Vol 2, No 2 (2007): (December 2007)" : 10 Documents clear
SPONGE MOLECULAR SCREENING FOR ANTIMICROBIAL GENES BY PCR Rosmiati Rosmiati; Emma Suryati; Andi Parenrengi; Sulaeman Sulaeman
Indonesian Aquaculture Journal Vol 2, No 2 (2007): (December 2007)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (160.132 KB) | DOI: 10.15578/iaj.2.2.2007.127-131

Abstract

Molecular biotechnology approach has been applied on sponge for preventingdiseases on fishery culture. This is important for anticipating and avoiding the using of amount of sponge in nature. The present study aims to screen the antimicrobial (oxytetracycline and chloramphenicol) genes of sponge. DNA extraction of samples was done using the DNeasy Plant mini kit, Phenol-Chloroform and modification of Phenol-Chloroform methods. The presence of oxytetracycline and chloramphenicol genes in sponge was detected using Polymerase chain reaction (PCR) technique. Result of the study showed that four species (Sylotella aurantium, Acanthella kletra, Gelliodes fibulatus and Auletta sp. were amplified for oxytetracycline and two species (Auletta sp. and Pericharax sp.) of sponge were amplified for chloramphenicol at each 226 bp.
EMBRYO DEVELOPMENT OF YELLOWFIN TUNA (Thunnus albacares) AT DIFFERENT INCUBATION TEMPERATURE Jhon Harianto Hutapea
Indonesian Aquaculture Journal Vol 2, No 2 (2007): (December 2007)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (461.839 KB) | DOI: 10.15578/iaj.2.2.2007.99-105

Abstract

The experiment was conducted in order to figure out the effect of incubation temperature on embryonic development of yellowfin tuna, Thunnus albacares eggs. Five different incubation temperatures were applied as treatments, i.e.: 24°C, 26°C, 28°C, 30°C, and 32°C with 3 replicate each. Ten micro plates with lid (IWAKI, Japan) were used; each has 6 well and 10 mL volumes. Five micro plates were used for experiment and five for balance on shaker. Three well of each micro plate were filled with 8 mL ultra violet sterilized sea water and 50 fertilized eggs. Temperature was set using Multi Thermo Incubator which has 5 level racks. Temperatures were set from the lowest to the highest on bottom to upper rack order. To maintain eggs dispersed in the medium, shaker on each rack was operated at 150 RPM. The embryo was monitored every 30-60 minutes depends on embryonic stage development using Microscope which was connected to Digital Camera DXM 1200F. Image analyses by Image Analyzer Program. The results showed, incubation temperature was significantly affect (P<0.05) embryonic development and hatching time of yellowfin tuna (Thunnus albacares) eggs. Optimum incubation temperature for embryo development and hatching was 28°C. Decreased on incubation temperature slows down embryo development at all stages, and vice versa, increased on incubation temperature accelerates embryo development.
TAXONOMY AND GENETIC RELATIONSHIPS OF PANGASIIDAE, ASIAN CATFISHES, BASED ON MORPHOLOGICAL AND MOLECULAR ANALYSES Rudhy Gustiano; Laurent Pouyaud
Indonesian Aquaculture Journal Vol 2, No 2 (2007): (December 2007)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (179.527 KB) | DOI: 10.15578/iaj.2.2.2007.107-112

Abstract

Pangasiids are economically important riverine catfishes generally residing in freshwater from the Indian subcontinent to the Indonesian Archipelago. The systematics of this family are still poorly known. Consequently, lack of such basic information impedes the understanding of the biology of the Pangasiids and the study of their aquaculture potential as well as improvement of seed production and growth performance. The objectives of the present study are to clarify phylogeny of this family based on a biometric analysis and molecular evidence using 12S ribosomal mtDNA on the total of 1070 specimens. The study revealed that 28 species are recognised as valid in Pangasiidae. Four genera are also recognized as Helicophagus Bleeker 1858, Pangasianodon Chevey 1930, Pteropangasius Fowler 1937, and Pangasius Valenciennes 1840 instead of two as reported by previous workers. The phylogenetic analysis demonstrated the recognised genera, and genetic relationships among taxa. Overall, trees from the different analyses show similar topologies and confirm the hypothesis derived from geological history, palaeontology, and similar models in other taxa of fishes from the same area. The oldest genus may already have existed when the Asian mainland was still connected to the islands in the southern part about 20 million years ago.
EFFECT OF DIFFERENT REMEDIATION TECHNIQUES AND DOSAGES OF PHOSPHORUS FERTILIZER ON SOIL QUALITY AND KLEKAP PRODUCTION IN ACID SULFATE SOIL AFFECTED AQUACULTURE PONDS Akhmad Mustafa; Jesmond Sammut
Indonesian Aquaculture Journal Vol 2, No 2 (2007): (December 2007)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2197.6 KB) | DOI: 10.15578/iaj.2.2.2007.141-157

Abstract

Acid sulfate soils (ASS) contain sufficient pyrite which, when oxidised following excavation for brackishwater aquaculture ponds, will generate acid and mobilise toxic metals. Production in affected ponds can be low due to poor growth of shrimp and fish, mass mortalities of stock and low plankton blooms. The resultant low soil pH can also cause poor klekap production due to the retention of phosphorus associated with elevated concentrations of Fe and Al in the pond soils. A series of experiments was conducted to determine the effects of different soil amelioration techniques and dosage of phosphorus (P) on soil and klekap production under laboratory conditions. The treatments consisted of two factors. The first factor tested was different techniques for ASS improvement (non-improvement, improvement through liming and improvement through remediation involving forced oxidation of pyrite, flooding and flushing of oxidation products). The second factor tested was phosphorus dosages, that is, with phosphorus and without phosphorus-based fertilizer. Each treatment had three replications. The experiment showed that liming and remediation had the same effect on several soil variables; they raised the soi pH (pHF, pHFOX, pHKCl) and decreased SPOS, Fe and Al. Remediation of ASS decreased retention of P and increased available-P of soil, whereas liming did not show a significant effect on retention of P and available-P in the doses used for this experiment. The interaction between the different soil improvement techniques and phosphorus fertilising showed a significant effect on klekap production with the highest klekap production of 23.21 mg/cm2 found in remediated soil and with a phosphorus fertiliser dosage of 75 kg/ha.
DIGESTIBILITY OF SELECTED FEED INGREDIENTS FOR TIGER GROUPER, Epinephelus fuscoguttatus Usman Usman; Kevin C. Williams; Mike A. Rimmer
Indonesian Aquaculture Journal Vol 2, No 2 (2007): (December 2007)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (103.496 KB) | DOI: 10.15578/iaj.2.2.2007.113-120

Abstract

The apparent digestibility (AD) of eight feed ingredients are widely available in Indonesia was determined. In each of two 5x5 latin-square experimental, tiger grouper Epinephelus fuscoguttatus juveniles (100—150 g) were fed a reference diet and four test diets in accordance with the latin-square design. Test feed ingredients were substituted at rates of 40% for animal meals or 30% for plant meals. Chromic oxide was used as the digestibility marker. In determining the ingredient AD, the substitution ratio was calculated as the proportion of the nutrient (or energy) contributed by the test ingredient on an ‘as-is’ basis. Digestibility tanks were steeply slope 200 L cylindroconical tanks with a bottom outlet to facilitate faecal collection, which was carried out at 3-hourly intervals throughout the day. Each collection period took 5—7 days with a similar acclimatization time between diets. A combined ANOVA of the data for both experimental showed no difference (P>0.05) in the AD’s for each reference diets. Thus for comparative purpose, the derived AD’s of the test ingredients were analysed as a single ANOVA. The digestibility of animal meals was generally high(>59% for dry matter, >83% for protein, >65% for lipid, and >70 for gross energy) while that of plant meals was slow (<53% for dry matter, <53% for protein, <66% for lipid, and <46% for gross energy). This information will enable grow-out feeds for tiger grouper to be formulated on a least-cost digestible nutrient basis.
FATTY ACID PROFILES OF CYCLOPOID COPEPOD NAUPLII Apocyclops panamensis AND THE EFFECTS OF SALINITY CHANGE Gede S. Sumiarsa; Ronald P. Phelps
Indonesian Aquaculture Journal Vol 2, No 2 (2007): (December 2007)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1277.118 KB) | DOI: 10.15578/iaj.2.2.2007.75-88

Abstract

Lipid and fatty acid profiles were described for copepod nauplii Apocy clops panamensis from fertilized brackish water ponds, and after being acclimated to fullsea water salinity. Mean total lipid content of copepod nauplii collected from ponds fertilized with inorganic fertilizer combined either with alfalfa meal, rice bran, wheat bran, and a combination of these fertilizers ranged from 5.66 ± 0.15 to 7.76% ± 0.27%. Non-polar (neutral) lipid fraction of pond copepod nauplii was a significantly higher percentage of the total lipid content (74.5 ± 1.8 - 93.5% ± 1.0%) compared to those of polar lipid (6.5 ± 1.0 - 21.3% ± 1.8%) (P= 0.000). DHA/EPA ratio in neutral lipids ranged from 1.8 ± 0.2 - 2.0 ± 0.1 with no significant differences in three fertilization regimes. DHA was 27.5% ± 0.56% of the neutral lipids and EPA 14.8% ± 0.8%. Acclimation of copepod nauplii for six hours from brackish to full-sea water salinity reduced their lipid content and individual dry weight significantly. Mean total lipid content was reduced 44.2%, non-polar lipid was reduced 46.9% and polar lipid was reduced 24.4%.Acclimation altered the DHA/EPA ratio, in the neutral fraction the ratio increased 26.3% but in the polar fraction it decreased 25%.
EFFECT AND COMPARISON OF RECYCLING AND STAGNANT FRESHWATER ON PERFORMANCES (GROWTH AND SURVIVAL RATES; FISH QUALITY) AND PROFITABILITY OF THE ORNAMENTAL FISH Barbus schwanefeldi (KAPIAT) REARED AT 4 DIFFERENT DENSITIES Sudarto Sudarto; Agus Priadi; Jack Slembrouck; Laurent Pouyaud; I Wayan Subamia
Indonesian Aquaculture Journal Vol 2, No 2 (2007): (December 2007)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (403.445 KB) | DOI: 10.15578/iaj.2.2.2007.159-162

Abstract

Comparing two different rearing systems in fish production through stagnant and recirculation water systems showed that recirculation system has several benefits such as reducing manpower, and minimize or eliminate in using antibiotics and also eliminate the grow out of diseases, increasing the profits due to increase in density of fish cultured in the system, and water quality remain stable in optimal condition.
EFFECT OF DIETARY CASSAVA MEAL ON GROWTH OF MUD CRAB, Scylla paramamosain Ketut Suwirya; Muhamad Marzuqi; I Nyoman Adiasmara Giri
Indonesian Aquaculture Journal Vol 2, No 2 (2007): (December 2007)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (148.774 KB) | DOI: 10.15578/iaj.2.2.2007.121-125

Abstract

It is widely recognized that a major constraint to development of a mud crabaquaculture industry is the availability and formulation nutritionally adequate but relatively low cost diets. Development of artificial diets, which seek to minimize inclusion of expensive feed ingredients such as fish and terrestrial meals, is considered to be a priority for improving the profitability of this emerging industry. Typically, carbohydrates such as starches are relatively cheap and therefore offer opportunity to supply dietary energy at low cost. The study examines the capacity of mud crab, Scylla paramamosain to utilize a range of dietary cassava meal as carbohydrate source. Four levels of cassava meal were used at inclusion levels of 0%, 10%, 20%, and 30% in diets. Mud crabs will readily accept the diet containing relatively high levels of cassava meal. This experiment proved that mud crab which fed 10% dietary cassava meal gains weight more than the one fed diet without dietary cassava meal. The increasing level of cassava meal to more than10% in diet reduced final weight and weight gain. To some extent, mud crab, Scylla paramamosain is capable to use dietary carbohydrate from cassava meal. The finding raises the possibility to include 10% cassava meal in formulation low cost diet for mud crab.
SINGLE-O-SHELTER HUNA (Cherax albertisi) AND REDCLAW (C. quadricarinatus) CULTURE Taufik Ahmad; Lilis Sofiarsih; Nuryadi Nuryadi; G. Apriyana
Indonesian Aquaculture Journal Vol 2, No 2 (2007): (December 2007)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (813.701 KB) | DOI: 10.15578/iaj.2.2.2007.89-97

Abstract

Many hatcheries successfully produced and sold cherax as ornamental crayfish. The attempt to culture cherax in earthen pond to produce consumable size yabbies facing the fact that cherax is a good hole digger and usually escapes through the hole in dyke. Single-o-shelter meant to provide shelter for every single spawner as well as hideout for the juvenile produced. The shelter for spawner was a 25 inches long and 2.0 inches diameter PVC pipe randomly spread on pond bottom. Aquatic weed (Vallisneria torta) grew in the shallow part of pond to provide hiding place for juvenile. The species stocked is huna and redclaw, each at density of 2 and 6 sets of spawner. One set of spawner consists of 3 males and 5 females weighing averagely around 20 g each. The experimental units are randomly selected to facilitate random block design in 2 rearing period as replicate. The pond dimension is 10x10 m, divide into 3 compartments i.e. feeding, ground, nursery ground and harvest ditch. Water depth at nursery ground was 30 cm and at the other compartments at 60 cm. Follow gravity force, the water in ponds flows at 50—100 L minute-1. Self-made diet distributed into pond twice a day to meet 3% daily feeding ration. Survival rate and specific growth rate of spawner as well as juvenile produced and number of gravid female checked at the end of each rearing period or every 3 months. After 6 months, average weight of redclaw and huna reaching 146.12 ± 34.47 g and 103.7 ± 29.83 g, respectively. Redclaw produced progeny of 5 size groups and huna produced only 2 groups. Respective to the species, average weight of the first offspring batch was 39.03 ± 5.33 and 26.83 ± 2.09 g. Redclaw at 2 sets of spawner and male grow faster than of 6 sets of spawner and female. No survival rate significant difference among ponds indicates that single–o-shelter technique provides sufficient shelter for spawner to grow and reproduce. Male monosex redclaw culture in earthen pond seems to be more promising than mixed-sex and female monosex culture for consumable size production of either huna or redclaw.
PRELIMINARY STUDY ON POPULATION DYNAMIC OF HARPACTICOID COPEPOD Euterpina acutifrons IN CULTURE CONDITION Philip Teguh Imanto; Gede S. Sumiarsa; Made Suastika
Indonesian Aquaculture Journal Vol 2, No 2 (2007): (December 2007)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (635.039 KB) | DOI: 10.15578/iaj.2.2.2007.133-139

Abstract

The most important factor to high mortality rate in larval rearing is feeding success in early larval stage related to kind and size of natural live food. Copepod basically is the main source of natural food in the open ocean having some advantages such as smaller size of nauplii, attractive movement and high nutritional value. Observation on population dynamic of harpacticoid copepod Euterpina acutifrons was carried out using 5-L plastic bucket with initial density 100 ind./L. Green algae Nannochloropsis sp. was added to culture media at density of 50,000 cells/mL as a basic feed and additional feeds given were wheat flour (group A) and chicken liver (group B) at a rate of 50 mg/bucket. The result showed that there was no difference on population pattern in both groups where the incubation time took eight days to hatch, from nauplii to the copepodite stage was three days and from copepodite to adult copepod took five-to-six days. The differences came up from population number: in group (A) the highest number of copepod-bearing-egg was only 133 ind., nauplii production up to 62,833 ind. and number of copepodites was 22,333 ind. lower compared to group (B) with the highest copepod-egg was 308 ind., nauplii was 113,333 ind. and copepodite was 51,167 ind. The conclusion pointed out that the kind of food did not influence population pattern (quality) but gave effect to population growth.

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