cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Fika Kharisyanti
Contact Email
fikakharisyanti@gmail.com
Phone
+6282232687366
Journal Mail Official
-
Editorial Address
Ruang Stem Cell, Gedung Lembaga Penyakit Tropis Lantai 2, Kampus C Universitas Airlangga
Location
Kota surabaya,
Jawa timur
INDONESIA
Journal of Stem Cell Research and Tissue Engineering
Published by Universitas Airlangga
ISSN : 26141264     EISSN : 26141256     DOI : https://dx.doi.org/10.20473/jscrte
Core Subject : Health, Science, Engineering,
Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary
Journal of Stem Cell Research and Tissue Engineering (JSCRTE) is published by Stem Cell Research and Development Center, Airlangga University. Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancerstem cells, developmental studies, genomics and translational research. Special focus of JSCRTE is on mechanisms of pluripotency and description of newly generated pluripotent stem cell lines. Articles that go through the selection process will be review by peer reviewer or editor. The journal is published regularly twice a year in December and May. Every publication consists of 60-70 pages and 5 scientific articles in the form of research, study literature, and the case study in English. The contributors Journal of Stem Cell Research and Tissue Engineering are Stem Cell researchers, lecturers, student and practitioners that came from Indonesia and abroad.
Arjuna Subject : Kedokteran - Imonologi dan Alergi
Articles 5 Documents
 1 
Search results for , issue "Vol. 6 No. 2 (2022): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING" : 5 Documents clear
TRIAMCINOLONE ACETONIDE EFFECT ON INFLAMMATORY RESPONSE AND EXPRESSION OF COLLAGEN TYPE I AFTER STRABISMUS SURGERY Andi Nur Ummah A. Nur Ummah
Journal of Stem Cell Research and Tissue Engineering Vol. 6 No. 2 (2022): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v6i2.42757

Abstract

Objective to determine the effect of triamcinolone acetonide as an antifibrotic agent on inflammatory response and collagen type I after strabismus surgery in rabbit Material and methods thirty six eyes of male New Zealand white rabbits divided by two groups, 18 rabbits eyes in control group and 18 rabbits eyes in treatment group. Control group underwent recess muskulus rektus superior and irrigation of balanced salt solution in reattachment site. Treatment group underwent strabismus surgery and irrigation Triamcinolone Acetonide (TCA) (Flamicort ® Dexa-medica)(40 mg/ml) 0,15 ml (6 mg). They were terminated on 15 postoperative days. Staining Hematoxylin & Eosin were performed to evaluate inflammatory response and immunohistochemistry using a monoclonal antibody Collagen I Alpha 2 Antibody (LS-C352030, LifeSpan BioSciences, Inc) was performed to evaluate collagen type I expression. Results this study showed of inflammatory response decreased and statistically significant in the treatment group (p=0.002, p=<0.05). Expression of type I collagen obtained a decrease in the treatment group compared to BSS group (p=0.004, p<0.05). Conclusion triamcinolone Acetonide (TCA) 40 mg/ml is one of therapeutic approaches that aims to reduce fibrosis after strabismus surgery by inhibiting the accumulation of inflammatory cells activation and suppressing of type I collagen deposition.
The EFFECT OF BEVACIZUMAB ON α-SMOOTH MUSCLE ACTIN EXPRESSION AND FIBROBLAST COUNT IN TRABECULECTOMY AREA TOWARDS PREVENTION OF FIBROSIS Sekar Ayu Sitoresmi Sekar Ayu
Journal of Stem Cell Research and Tissue Engineering Vol. 6 No. 2 (2022): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v6i2.42759

Abstract

Objective to analyze the effect of bevacizumab on α-smooth muscle actin expression and fibroblast count in trabeculectomy area of rabbit models in order to find a safer modulator of wound healing to improve surgical outcome. Material and methods 16 New Zealand white rabbits aged 4-6 months and weigh between 2,5-3,5 kg were performed trabeculectomy in their right eyes with postoperative subconjunctival injection of BSS and Bevacizumab. Rabbits were put into control and bevacizumab group using simple random sampling. Examination were done postoperative day 1, 7, and 14 and subjects were terminated and performed enucleation on postoperative day 14. The samples were histologically stained with Haematoxyline-Eosin to count the fibroblast and immunohistochemistry using α-smooth muscle actin antibody to differ the myofibroblast from fibroblast and the expression of α-SMA were collected using immunoreactive score. Result Mann Whitney u test and independent t-test were used to analyze the data, and we found both less expression of α-smooth muscle actin and fibroblast count on bevacizumab group compared to control group which indicates less myofibroblast, fibroblast, and less scarring potential in trabeculectomy area. Conclusion bevacizumab inhibits fibroblast proliferation and its differentiation to myofibroblast that lead to less collagen production and fibrosis.
The EFFECT OF BEVACIZUMAB ON ANGIOGENESIS INTRABECULECTOMY AREA Diana Yuliawati
Journal of Stem Cell Research and Tissue Engineering Vol. 6 No. 2 (2022): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v6i2.42773

Abstract

Objective to examine the effect of Bevacizumab injection to the angiogenesis which the amount and density of blood vessel as the indicators after trabeculectomy procedure. Method This was a true experimental study using 16 eyes of 16 New Zealand White Rabbit eye treated by trabeculectomy procedure with eight eyes as the control group using Balanced Saline Solution (BSS) and eight eyes as the treatment group using Bevacizumab. It was injected subconjuctiva after the trabeculectomy. At the end of the study all rabbits in each group was sacrified, the eye was enucleated and the bleb area was dissected, and then processed for histological studies. The amount and density of blood vessel were evaluated using haematoxyllin eosin methode at day 14 after the eyes was done for trabeculectomy procedure. Result The mean of amount of blood vessel in control group was 22,63 ± 11,02 and treatment group was 14,75 ± 4,92 (p=0.043). The mean of density of blood vessel in control group was 19,10 ± 1,69 % and treatment group was 16,53 ± 2,90 % (p=0.029)%. The result shows there were statistically significant difference between the two groups (p<0.05). Conclusion In this study the subconjunctival Bevacizumab injection after trabeculectomy reduce the amount and density of blood vessel compared with subconjunctival BSS injection only, thus it is potential in preventing subconjunctival fibrosis after trabeculectomy
The EFFECT OF Anti-miRNA 144 AND Anti-miRNA 150 ON THE EXPRESSION OF α GLOBIN CHAINS IN PBMC Rini Riyanti
Journal of Stem Cell Research and Tissue Engineering Vol. 6 No. 2 (2022): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v6i2.42774

Abstract

The excess of unbound α globin chains are the basic pathophysiology of the cause of clinical symptoms in major β thalassemia. Recently there are many alternative therapies by increasing γ globin chains to reduced the effects of unbound α globin chains. Alternative therapies by decreasing α globin chains have not been much noticed. α globin expression involves complex regulatory involving transcription factors and miRNAs. It involves GATA-1, KLFD and MYB transcription factors . It also involves miRNA-144 and miRNA-150. The role of miRNA-144 and miRNA-150 to reduce the α globin chains expression in major β thalassemia patients is an alternative therapy. miRNA-144 and miRNA150 activity need to be known by using anti-miRNA 144 and anti- miRNA 150. Analyzed the effect of anti-miRNA 144 and anti-miRNA 150 on the expression of α globin chains. This study was an experimental study using PBMC of a major β thalassemia patients. PBMC divided into four groups that were not transfected, transfected by anti-miRNA 144, transfected by anti-miRNA 150 and transfected by anti-miRNA 144 and anti-miRNA 150. qPCR examinations to find out expression of miRNA-144 and miRNA-150. Western blot examination to find out the expression of α globin chains. There was significantly lower miRNA-144 expression in PBMC of major β thalassemia patients who had been transfected by anti-miRNA 144 than those not transfected. In line with the result of miRNA-144 expression which was 0.17 times lower than the control group. There was significantly lower miRNA-150 expression in PBMC of major β thalassemia patients who had been transfected by anti-miRNA 150 than those not transfected. In line with the result of miRNA-150 expression which was 0.30 times lower than the control group. There was significantly lower α globin chains expression in PBMC thalassemia patients who had been transfected by anti-miRNA 150 than those not transfected. There was significantly lower α globin chains expression in PBMC thalassemia patients who had been transfected by anti-miRNA 150 and anti-miRNA 144 than those not transfected. This is evidenced by the decreased in the area 10-25 KDa band. Based on this study, the administration of anti-miRNA 150 or anti-miRNA 144 and anti-miRNA 150 is capable of decreasing the expression α globin chains in PBMC of major β thalassemia patients.
BEVACIZUMAB EFFECT ON EXPRESSION OF COLLAGEN TYPE I AFTER TRABECULECTOMY Shinta Shinta Arta Wiguna
Journal of Stem Cell Research and Tissue Engineering Vol. 6 No. 2 (2022): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v6i2.42836

Abstract

Objective to determine the effect of bevacizumab as an antifibrotic agent on collagen density, collagen thickness and collagen type I after trabeculectomy in rabbit. Materials and methods : Sixteen male New Zealand white rabbits divided by two groups, 8 rabbits in control group and 8 rabbits in treatment group. Control group underwent trabeculectomy and injection of balanced salt solution. Treatment group underwent trabeculectomy and subconjunctival injection of bevacizumab (1.25mg, 25mg/mL). They were terminated on 14 postoperative days. Masson Trichrome were performed to evaluate collagen collagen density, collagen thickness. Immunohistochemistry using a monoclonal antibody to collagen type I was performed to evaluate collagen type I expression. Results This study showed the density of collagen fibers decreased and statistically significant in the treatment group (p = 0.075, p 0.05). Expression of type I collagen obtained a decrease in the treatment group compared to BSS group (p = 0.006, p<0.05). Conclusion Bevacizumab reduces bleb fibrosis by inhibition of angiogenesis and accumulation of extracellular matrix. Postoperative subconjunctival injection of bevacizumab may limiting scar tissue formation at the site of trabeculectomy by blocking collagen synthesis.

Page 1 of 1 | Total Record : 5

 1 

Filter by Year

2022 2022


Reset
Filter By Issues
All Issue Vol. 7 No. 1 (2023): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING Vol. 6 No. 2 (2022): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING Vol. 6 No. 1 (2022): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING Vol. 5 No. 2 (2021): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING Vol. 5 No. 1 (2021): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING Vol. 4 No. 2 (2020): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING Vol. 4 No. 1 (2020): Journal Of Stem Cell Research and Tissue Engineering Vol. 3 No. 2 (2019): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING Vol 3, No 2 (2019): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING Vol. 3 No. 1 (2019): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING Vol. 2 No. 2 (2018): Journal of Stem Cell Research and Tissue Engineering Vol. 2 No. 1 (2018): Journal of Stem Cell Research and Tissue Engineering Vol. 1 No. 1 (2017): Journal of Stem Cell Research and Tissue Engineering More Issue