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INDONESIA
HAYATI Journal of Biosciences
Published by Institut Pertanian Bogor
ISSN : 19783019     EISSN : 20864094     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences
HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, animal and human. HAYATI J Biosci has been also indexed/registered in Crossref, DOAJ, CABI, EBSCO, Agricola and ProQuest.
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Articles 20 Documents
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Search results for , issue "Vol. 30 No. 5 (2023): September 2023" : 20 Documents clear
Development of Secondary Forest Succession Based on Estimation of Forest Carbon Stocks Ten Years Post-Merapi Volcano Eruption Inggita Utami; Anjar Dimara Sakti; Febriant Isabella Yusuf; Fahmiatul Husna; Denni Susanto
HAYATI Journal of Biosciences Vol. 30 No. 5 (2023): September 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.5.834-842

Abstract

The eruption of the most active volcano in Indonesia, Mount Merapi, has resulted in a very dynamic landscape as a form of ecosystem succession. This study aimed to analyze the carbon stock and vegetation composition in the secondary forest with variations in the level of disturbance after the 2010 eruption of Mount Merapi. Data collection was carried out in January 2020, in which biomass, necromass, and soil organic carbon data were taken in plot transect and Geographic Information Systems carbon stock products compared field data. The results showed that secondary forests at stations A and B with low and medium disturbance levels had carbon stocks in the high category. In contrast, station C at Cangkringan Resort, with high disturbance levels, still had carbon stock with a low category. Furthermore, the comparison of 2020 field data with GIS carbon stock products concluded a similar pattern between carbon stock from aboveground biomass WHRC 2010 and belowground biomass NASA 2010. This research concludes that the disturbance affected forest carbon stocks ten years after the 2010 Merapi eruption. Therefore, monitoring the vegetation community needs to be carried out annually through a combination of spatial and direct checks in the field.
Taxonomic Profiling of Microorganisms Inhabiting Two Solar Salterns that Produce High- and Low-Quality Salts Ekowati Chasanah; Asri Pratitis; Rini Susilowati; Agus Heri Purnomo; Hedi I. Januar; Agustinus R. Uria
HAYATI Journal of Biosciences Vol. 30 No. 5 (2023): September 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.5.957-984

Abstract

The contribution of halophilic microorganisms to the quality of salts produced in solar salterns has recently been recognized but not clearly understood. Using metagenomic 16S rRNA gene sequencing approach, we showed the microbial composition difference between the Tuban crystallization pond (CP-Tuban) that produces low-quality salt and CP-Sampang as a representative solar saltern that yields high-quality salt. Dominant classes in both traditional salterns were represented by γ-proteobacteria and halobacteria that occurred at higher prevalence in CP-Sampang. Microbial taxa, including beneficial genera, in CP-Sampang were more diverse and abundant compared to CP-Tuban. Among 180 genus-level OTUs identified in CP-Sampang, 127 of them were considered unique due to their absence in CP-Tuban. Higher levels of dissolved oxygen (DO) and nutrient (phosphate, nitrate, and ammonia) in the seawater reservoir (SR) of Sampang may contribute to more diverse phytoplankton genera, which could support the growth of beneficial heterotrophic microbes that positively affect the salt quality of the CP-Sampang. Low number of Dunaleilla sp. in both CPs do not seem to influence the quality of salts produced. The outcome of these comparative studies provides new insights into the contribution of diverse microbial taxa in correlation with physico-chemical parameters and phytoplankton communities to the high quality of salts produced in traditional solar salterns. The presence of beneficial genera in the enriched microbial cultures could provide an important basis for further applications, such as improving the quality of salt produced and producing unique compounds and enzymes.
Optimization of Biomass and Secondary Metabolite Production in Gynura procumbens (Lour.) Merr. Adventitious Roots Culture by Using the Method of Subculture and Fed-batch Cultivation in a Bioreactor Dannis Yuda Kusuma; Alfinda Novi Kristanti; Anjar Tri Wibowo; Boon Chin Tan; Yosephine Sri Wulan Manuhara
HAYATI Journal of Biosciences Vol. 30 No. 5 (2023): September 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.5.797-807

Abstract

The valuable extract of bioactive compounds from Gynura procumbens has been widely manufactured into various health products. The demand for these compounds is continuously increasing, but production through conventional farming methods is insufficient due to limited agricultural land and environmental stresses. An alternative to producing plant biomass is in vitro cultivation methods. This method requires less space and enables biomass propagation in a controlled condition that can facilitate stable and efficient production of plant secondary metabolites. This study evaluated the effect of inoculum subculture periods and culture methods on G. procumbens biomass and secondary metabolite production in a bioreactor. The 3-L airlift balloon type-bubble bioreactors was modified in this study to adopt the treatment of 1st-5th subculture periods and fed- and batch-cultivation strategies. We found the G. procumbens adventitious root culture was optimally derived from the 1st subculture produced biomass of 148.02±1.45 g FW and 8.59±0.12 g DW, and TPC (14.48±1.08 mg GAE/g DW) and TFC (116.89±0.44 mg KE/g DW and 33.97±0.13 mg QE/g DW). Additionally, the fed method after 28 days of culture using double distilled water replenishment improved adventitious root biomass (213.75±35.00 g FW and 11.21±0.18 g DW), while nutrient replenishment improved TFC (52.14±0.44 mg KE/g DW and 14.54±0.13 mg QE/g DW). These results can be used to optimize the cultivation of G. procumbens adventitious roots in a large-scale bioreactor.
Phylogenetic Analysis of Cyclocheilichthys apogon and Cyclocheilichthys armatus (Fish: Cyprinidae) from West Sumatra Dewi Imelda Roesma; Djong Hon Tjong; Syaifullah; Dyta Rabbani Aidil
HAYATI Journal of Biosciences Vol. 30 No. 5 (2023): September 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.5.895-906

Abstract

Cyclocheilichthys apogon (Beardless Barb fish) and Cyclocheilichthys armatus (Hihgfin Barb fish) in West Sumatra is known by the local name Turiak and Catua fish, respectively, are economically important species. Reducing populations and limited biological information about these fishes make their genetic diversity and phylogenetic relationships crucial. The genetic variation was investigated by analyzing the sequencing of a part of mitochondrial DNA Cytochrome b (Cyt b) and Cytochrome oxidase-I (COI) genes of 23 individuals collected from West Sumatra populations. Phylogenetic analysis revealed the monophyletic lineage of the Cyclocheilichthys group and supported Anematichthys as the synonym of Cyclocheilichthys. C. armatus was closely related to C. janthochir and separated from C. apogon with sequences divergence were 12.7-14.0% (Cyt b) and 9.4%-10.0% (COI). C. apogon and C. armatus have low haplotype diversity and genetic diversity, respectively. Genetic diversity within and among populations with sequences divergence was 0.0-0.8% (Cyt b) and 0.0%-0.7% (COI), respectively. The low-level diversity may signify a possibility of gene flow within and among populations. The current data suggests all populations as a single stock unit. Present genetic information is important to establishing effective population management, conservation, and breeding strategies for Turiak and Catua fish.
Distribution Analysis of Asiatic Palm Weevil Rhynchophorus vulneratus Panzer (Coleoptera: Dryphthoridae) using GIS Technique and the Interaction with Coconut Beetle Oryctes rhinoceros L. (Coleoptera: Scarabaeidae) Hanindyo Adi; R.C. Hidayat Soesilohadi; Andhika Puspito Nugroho; Suwarno Hadisusanto; Sudaryatno; Abdulrahman Saad Aldawood; Sukirno Sukirno
HAYATI Journal of Biosciences Vol. 30 No. 5 (2023): September 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.5.918-926

Abstract

Asiatic palm weevil Rhynchophorus vulneratus Panzer is one of the key pests of coconut in Indonesia. Information regarding the spatial distribution of R. vulneratus is needed to support Integrated Pest Management and can be analyzed using Geographic Information System (GIS). Initial studies on the interaction between R. vulneratus and Oryctes rhinoceros were also studied in this study. This research aimed to analyze the spatial distribution of R. vulneratus using GIS and its interaction with the Coconut beetle Oryctes rhinoceros. The research was conducted in Yogyakarta as a habitat model (0-1,000 masl). R. vulneratus and O. rhinoceros were catched by installing aggregation pheromone traps hanging to coconut plants at 1.7 m above ground. The research was conducted during the rainy and dry seasons. The spatial distribution of R. vulneratus was analyzed by IDW interpolation using QGIS 3.22, whereas its interaction with O. rhinoceros was analyzed by the Pearson correlation test using SPSS 22. The results showed low number of R. vulneratus captured in the Yogyakarta area during the rainy season at 0-1,000 masl. However, the number of R. vulneratus during the dry season has increased, and the distribution rate was dominated by medium to very high levels, especially at an altitude of 0-300 masl. The study also showed that the number of R. vulneratus was not influenced by the number of O. rhinoceros, which suggested that the abundance of O. rhinoceros cannot accurately predict the abundance of R. vulneratus.
Genetic Diversity of Gracilaria spp. in the Intertidal Zone on the South Coast of Yogyakarta, Indonesia Based on DNA Barcoding with rbcL Marker Feni Susanti; Ratih Ida Adharini; Dini Wahyu Kartika Sari; Eko Setyobudi
HAYATI Journal of Biosciences Vol. 30 No. 5 (2023): September 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.5.907-917

Abstract

Gracilaria spp. is a commercial seaweed utilized in various food, pharmaceutical, and health industries. Due to the high plasticity of seaweed, morphological identification must be validated by molecular identification using DNA barcoding. This study aims to identify the genetic diversity of Gracilaria spp. based on DNA barcoding with an rbcL marker. Samples were collected from six beaches, i.e., Trenggole, Drini, Siung, Wediombo, Nguyahan, and Ngedan, from September-November 2021. The methods in this study were sampling, sample preservation, morphological and tissue observations, DNA extraction, PCR and electrophoresis, and data analysis. The results showed four species of seaweed based on the rbcL marker: Gracilaria sp., G. salicornia, G. edulis, and G. vieillardii. Based on 18 samples confirmed by phylogenetics, five different species were found, namely G. salicornia, G. edulis, G. vieillardii, Gracilaria sp. 1, and Gracilaria sp. 2 The last two species, i.e., Gracilaria sp. 1 and Gracilaria sp. 2, from the intertidal zone of the south coast of Yogyakarta may have never been reported to GenBank.
Macroarthropod Diversity, Distribution, and Community Structure in Cikarae Cave of the Klapanunggal Karst, West Java Isma Dwi Kurniawan; Cahyo Rahmadi; Rahmat Taufiq Mustahiq Akbar; Omar Calva; Fezaa Ashelia Zakia Ameliee; Ahmad Zaenal Ependi
HAYATI Journal of Biosciences Vol. 30 No. 5 (2023): September 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.5.995-1007

Abstract

Arthropods are the most dominant fauna in cave realms. Ecological studies of cave-dwelling arthropods are essential for cave ecosystem conservation. This study was conducted to determine the ecological aspects of macroarthropods in Cikarae Cave, West Java, focusing on their diversity, distribution, and community structure. The cave passage was divided into three zones based on environmental disparities. Data collection was carried out through direct search and counting methods. Data were analyzed using non-metric multidimensional scaling (NMDS) and several ecological indices (diversity, evenness, and dominance). Nineteen macroarthropod morphospecies were recorded and distributed among 5 classes, 11 orders, and 18 families. Most of these morphospecies were troglophiles. A new troglobitic species (Isopoda: Philosciidae) with a high degree of troglomorphy and exclusive microhabitat was registered. Most morphospecies were collected in Zone 1 (17), followed by Zone 3 (9) and Zone 2 (8). Collected macroarthropods preferred cave walls over floors and ceilings. Overall, Cikarae showed a low diversity index (0.782) and evenness (0.265), while dominance was relatively high (0.692). Trachyjulus tjampeanus, Rhaphidophora sp., and Theridiosomatidae sp. were the most dominant taxa.
Effect of the Supplementation of Endogenous Probiotics in Feed on Growth Performance, Digestive Enzyme Activity, and Non-Specific Immunity Gene Expression of Sandfish, Holothuria scabra Juveniles Sari Budi Moria Sembiring; Jhon Harianto Hutapea; Ketut Mahardika; I Nyoman Adiasmara Giri; Rarastoeti Pratiwi; Haryanti; Suwarno Hadisusanto
HAYATI Journal of Biosciences Vol. 30 No. 5 (2023): September 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.5.808-815

Abstract

The present study aimed to evaluate the effects of endogenous compared to commercial probiotics on the growth, digestive enzyme activity, and non-specific immunity of sandfish juveniles. Treatments were: A. diet without probiotic; B. diet supplemented with endogenous probiotics Gamma proteobacterium strain M-4, Bacillus subtilis strain Q-1, Bacillus sp. strain E-2; C. diet supplemented with a commercial probiotic containing B. subtilis, B. licheniformis, and B. pumilus. The research was conducted for 4 months using 12 plastic containers, 30 juveniles each (initial weight of 2.72±0.37 g, mean ± standard deviation). From each container, ten samples were taken randomly every 14 days and weighed and three intestine samples at initial and every following month to analysis enzyme activities. At the end the study, all juveniles were counted and weighed individually, and performed a challenge test by injected a 106 CFU ml-1 Vibrio azureus strain 4C-1 at 0.1 ml/ind. Immune response observed were: total coelomate and gene expression (SOD, CAT, LZM) by Quantitative real-time PCR. The specific growth rate of juveniles fed by diet B was significantly different from treatment A (P<0.05), its final weight was higher compared to diets A and C (P<0.05). Protease, lipase, and amylase activity in treatment B was higher compared to C and A (P<0.05). The survival rate, total coelomocyte count, gene expression after being challenged were higher in treatment B compared to C and A (P<0.05). The present study showed endogenous probiotics promote growth, improve digestion enzyme activity, and stimulate non-specific immune responses.
Red Betel Leaf Bioactive Compounds as ERα Receptor Inhibitors In Silico and MCF-7 Cell Anticancer In Vitro Ayu Tri Nursyarah; Mega Safithri; Dimas Andrianto
HAYATI Journal of Biosciences Vol. 30 No. 5 (2023): September 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.5.789-796

Abstract

Cancer is one of the leading causes of death in the world. Excess endogenous estrogen is a risk factor for breast cancer. Red betel leaf herbal plants have been used as an alternative cervical and colon cancer treatment. This study aimed to obtain the active compounds that play a role in ERα receptor inhibitors in silico and to determine the anti-breast cancer cytotoxic activity of the extract and fraction of red betel leaf against MCF-7 cells. We use in silico research method using the YASARA Structure software with anti-breast cancer receptors, namely 3ERT, and in vitro using the MTT test on the anti-breast cancer cytotoxic activity of MCF-7 cells. There are 38 compounds that were obtained from the research. The results of the in silico test showed that the bioactive compound that played a role in inhibiting the ERα was 2-(4-Hydro xyphenyl)-2-phenyl-N(3,3-diphenylpropyl)-acetamide (44578655), a compound from water fraction, with an inhibition constant of 2.82 × 10-8 µM and Gibbs free energy of –10.2880 Kcal/mol. In vitro results showed that the best cell growth inhibition value was obtained from the n-hexane fraction at a concentration of 500 ppm of 73.42%. The conclusions of this study indicate that the bioactive compound of red betel leaf is water fraction is the best fraction inhibition. However, the hexane fraction proved to have cytotoxic activity against breast cancer MCF-7 cells.
The Effect of Cryopreservation on Cytochrome Oxidase1 (CO1) Gene and the Relationship with Spermatozoa Motility of Albino Pangasius catfish (Pangasionodon hypophthalmus) Uswatun Hasanah; Abinawanto; Alimuddin; Arief Boediono; Eni Kusrini
HAYATI Journal of Biosciences Vol. 30 No. 5 (2023): September 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.5.825-833

Abstract

Cryopreservation is a technique for storing cells and tissues at very low temperatures for the possible usage of the stored cells and tissues throughout the year. Sperm cell cryopreservation in some species causes a decrease in sperm quality and DNA damage. Inappropriate cryopreservation protocols can cause changes in sperm physiology. Mitochondria are organelles that play a role in producing energy for sperm motility. Mitochondria have DNA molecules with small sizes compared to structures from nuclear DNA. This study analyzed the effect of cryopreservation on sperm motility and the Cytochrome Oxidase1 (COI) gene. The CO1 gene in mitochondrial DNA plays a role in energy production for spermatozoa motility. The cryopreservation was performed using skim milk and 10% methanol cryoprotectant, and the temperature in the equilibration process was 4-5°C for 10 minutes. Cryopreservation took place for 14 days in the freezer at -80°C. In addition, the thawing process was performed for 1-2 minutes at 40°C. This study found that the number of lesions per 10 kb in the CO1 gene in post-equilibration spermatozoa was (9.24±3.74), and post-thawing spermatozoa (was 10.26±7.54). Spermatozoa motility was obtained in fresh spermatozoa, i.e., 87±1.5%, post-equilibration spermatozoa 79±4.5%, and post-thawing spermatozoa 30±3.2%. This study concluded that cryopreservation of spermatozoa causes CO1 gene lesions and that in cryopreserved spermatozoa, there is a decrease in spermatozoa motility compared to fresh spermatozoa.

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