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Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Published by Universitas Gadjah Mada

ISSN : 01261312 EISSN : 23563931 DOI : http://dx.doi.org/10.19106/JMedSci005303202101

Core Subject : Science,

Immunology & microbiology Neuroscience

Journal of the Medical Sciences (JMedSci) or Berkala Ilmu Kedokteran (BIK) is an international, open-access, and double-blind peer-reviewed journal, published by Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada Yogyakarta Indonesia. JMedSci aiming to communicate high-quality articles in the areas of biomedical science from basic to clinical sciences.The journal welcomes papers from original articles, case reports, reviews, and book reviews. All papers published in JMedSci are freely available as downloadable pdf files. The journal began its publication on March 1973 and published quarterly (January, April, July, and October). JMedSci is abstracted and indexed in DOAJ, Crossref, Google Scholar, Sinta, Indonesia One Search. JMedSci is accredited by Directorate of General Higher Education, the Ministry of Research, Technology, and Higher Education, Indonesia

Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)

Articles 12 Documents

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Search results for , issue "Vol 39, No 03 (2007)" : 12 Documents clear

ytotoxic effect of n-hexane insoluble fraction obtained from kloroform extract of kembang bulan leaves [Tithonia diversifolia (Hemsley) A. Gray] on HeLa cells and mechanism detection by Hoechst 33342 Zany sari Vindawati, Rita Rakhmawati, Mae S.H Wahyuningsih, Rul Afiyah Syarif,
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 03 (2007)
Publisher : Universitas Gadjah Mada

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Background: Tithonia diversifolia (Hemsley) A.Gray is a plant traditionally used to cure various diseases. The previous study indicated that n-hexane insoluble fraction of chloroform extract of T. diversifolias leaves showed cytotoxic effect on Hela cells. Separation of n-hexane insoluble fraction into several fractions was conducted in order to simplify the components. Therefore, compounds responsible for cytotoxiceffect and their mechanism of action can be determined. .Objectives: To determine fraction having the most cytotoxic effect and its mechanism of action obtained from n-hexane insoluble fractions of T.diversifolia leaves on Hela cells.Materials and methods: Fractionation of n-hexane insoluble fraction from chloroform extract of T. diversifolia leaves was done by using vacuum liquid chromatography. By MTT assay, the cytotoxic effect of fractions on Hela cells in vitro was determined. and doxorubicin was used as positive control. Each group was tested for 3 times In = 3) and the growth inhibition percentage of Hela cells was counted. The data was analyzed by probit regression, and mechanism of action of the active fraction is conducted by staining with hoechst 33342.Results: Fractionation gave five fractions IF1-5). The ICso levels obtained of those fractions were : F1 having ICso = 32.168 :!: 12.513; F2 = 11.416 :!: 0.535; F3 III = 6.223 :!: 1.303; F4 = 94.308 :!: 7.843; and F5 = 41.740 :!: 6.381Ig/ml; while the ICso value of doxorubicin was 0.241 :!: 0.170 Iglml. Conclusions: Fraction 3 of the n-hexane insoluble displayed the highest cytotoxic effect on Hela cells with ICso= 6.223:!:: 1.303 Iglml. Fraction 3 at the concentration of 3.93 uglml caused chromatin condensation and fragmentation of DNA on Hela cells.Key words: T. diversifolia, cytotoxic effect. Hela cells, insoluble n-hexane fractions

Nonmosaic 45, XO karyotype in a woman with Turner syndrome without any cognitive, psychosocial or behavioral deficiencies (A Case report) Djaswadi Dasuki, Enny S Pamuji
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 03 (2007)
Publisher : Universitas Gadjah Mada

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A 22 year old woman with primary amenorrhea came to The Reproductive Endocrinology and Infertility Centre, University of Gadjah Mada, Dr.Sardjito Hospital. She had no abnormalities in cognitive, psychosocial or behavioral capacities, she had almost completed her university degree. On examination she had no secondary sexual development, no pathognomonic abnormal phenotype related to abnormal karyotype. There was no indication of family history associated ,;pecifically to the abnormal karyotype, phenotype feature of Turner syndrome and a specificThe patient karyotype analysis of blood sampling indicated 45XO, and it failed to demonstrate any mosaicism. Laparoscopic examination revealed a hypoplastic-lobulated-unicorns uterus, as streak gonad at the left side and no ovary at the other side.Keywords: Turner syndrome, non behavioral deficiencies. nonmosaic 45, XO karyotype, normal hormoneÂ level

Relation between vitamin C and vitamin E levels with malondialdehyde in elderly Irianti Puji Lestari, Prasetyastuti Sunarti, Dyah Arum Retnaningstyas
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 03 (2007)
Publisher : Universitas Gadjah Mada

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Background: Aging is a natural process during life. One of the aging theories is related to free radical production. Increase of free radical production can trigger lipid peroxidation that result in peroxide lipid such as malondialdehyde (MDA). The lipid peroxidation can be inhibited by body defence system such 8S vitamin C and vitamin E.ObJective: to understand the relation between plasma vitamin C, vitamin E and MDA levels in elderly Method: This was an observational research with cross sectional design. The subjects were thirty two elderly who live in Pakem district, Sleman. Determination of vitamin C and peroxide lipid level used spectrophotometer, whereas vitamin E level was determined by spectrofluorometer.Reaulta : The concentration of vitamin C , vitamin E and MDA of the subjects were 0.50 + 0.26 mglml, 26.38 ..:!:. 7.95 pg/ml and 0.23 ..:!:. 0.13 nmol/ml respectively. The correlation of both vitamin C vs MDA and vitamin E vs MDA were not significant, r = -0.012 and -0,108, respectively.Conclualon: There were no correlation between vitamin C, vitamin E and MDA levels.Keyword: elderly ; free radical;vitamin C; vitamin E; malondialdehyde.

Expression of CD5, CD1 0, Bcl-2 and Bcl-6 in diffuse large B cell lymphoma based on International Prognostic Index Guntersah, Indrawati Tody
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 03 (2007)
Publisher : Universitas Gadjah Mada

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Background: Diffuse large B cell lymphoma is the most common type of non-Hodgkin lymphoma. This tumor has heterogeneous clinical feature, morphology, genetic and molecular alterations. Diffuse large B cell lymphoma with a germinal centre ( GC ) pattern of gene expression including CD10 and Bcl-6 has a more favorable outcome. CD5 and Bcl-2 expression has been shown to have an adverse effect on the outcome in diffuse large B cell lymphomaObjective: To investigate the difference of CD5, CDlO, Bcl-2 and Bcl-6 expression based on International Prognostic Index IIPI) in diffuse large B cell lymphomaMethods: Fourty five cases of B cell diffuse large B cell lymphoma from embedding parafin tissue were constituted the basis of the study. Immunohistochemical examination using CD20, CD5, CD10, Bcl-2 and Bcl-6 monoclonal antibody was done. The difference of CD5, CD10, Bcl-2 and Bcl-6 expression between high and low IPI of diffuse large B cell lymphoma were analized by chi square testResult: There Vlias significant difference between the number of cases with positivity of CD5 and CD10 expression in high and low IPI of diffuse large B cell lymphoma. The result supported that immunophenotyping related B cell differentiation can help to predict clinical behavior and prognosis in diffuse large B cell lymphoma.Conclusion: Conclusion: There was significant difference between the number of cases with positivity of CD5 and CD10 expression in high and low IPI of diffuse large B cell lymphoma. The result supported that immunophenotyping related B cell differentiation can help to predict clinical behavior and prognosis in diffuse large B cell lymphoma.Key words : diffuse large B cell lymphoma, CD5, CD 10, IPI

he correlation between HPV infection and p53, pRb, KI-67 as well as tunnel expression of cervical carcinoma Endang Soetristi, Irianiwati Ahmad Ghozali
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 03 (2007)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Background: HPV infection has an important role in cell proliferation and apoptosis of malignant neoplasia through the binding of their E6 and E7 protein to the p53 and pRb. In cervical cancer the role of HPV infection in cells proliferation and apoptosis has not yet been defined up to now, since mutation of p53 gene and Rb gene in cervical cancer is very rare.Methodes: Thirty-eight block parafin cases of squamous cell carcinoma of the cervix were stained immunohistochemically with polyclonal antibody against HPV and monoclonal antibody against p53, Rb, Ki-67 and Tunnel. with chromogen DAB and counterstain Hematoxyllin Meyer. The expression of HPV, p53, pRb, Ki-67 and Tunnel were counted based on the number of positive cells/100 tumor cells in five microscopic fields. The correlation between HPV expression and p53, pRb, Ki-67, Tunnel expression respectively were analyzed with Pearson correlation analysis.Results: There was a significant correlation between HPV expression with either p53 expressionR: 0.491, P:0.002) and or Ki-67 expression (R:0.397, P:0.014), but there was no significant correlation between HPV expression with pRB expression (R:0.3000, P:0.067) and Tunnel expression ( R:O,888, P:0,599).Conclusion: This research showed that HPV infection had important role in cell proliferation but not in cell apoptosis of cervical cancer.Key words: HPV, proliferation, apoptosis, p53, pRb, Ki-67, Tunnel, cervical carcinoma

Comparasion of Sport Urine Protein Creatinine From Three Different Time Urine Samples in Children With Primary Nephrotic Pungky Ardani, Jusuf Hartanto Mohammad Juffrie
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 03 (2007)
Publisher : Universitas Gadjah Mada

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Background: First voiding of morning urine protein/creatinine ratio on a single voided urine sample correlates well with 24 hour urine collection and can be used to detect and monitor proteinuria in children. Objective: Aim of this study was to determine whether the random urine samples can be used as reliable indicator for spot urine protein/creatinine ratio.Methods: 40 primary nephrotic syndrome children aged 1-16 years admitted to the Department of Pediatric, Dr Sardjito hospital were included in this study. Urine samples from three different times were collected: first voiding of morning urine, noon voiding and night voiding. The three samples were analyzed for protein level, creatinine level and protein/creatinine ratio of spot urine. The protein/creatinine ratio was compared and correlation was calculated according to the three different time collections.Results: There was no significant difference of noon and night protein/creatinine ratio compared with first voiding of morning urine protein creatinine ratio (p> 0.05). The correlation of protein/creatinine ratio between the three samples was significantly high (r=0.95, 0.96 and 0.98, p < 0.01).Conclusions: For quantifying proteinuria, there is convincing evidence that the random urine samples can be used to predict the level of protein excretion as measured in a 24-hour sample.Keywords: Proteinuria, protein, creatinine ratio, primary nephrotic syndrome

ytotoxic effect of n-hexane insoluble fraction obtained from kloroform extract of kembang bulan leaves [Tithonia diversifolia (Hemsley) A. Gray] on HeLa cells and mechanism detection by Hoechst 33342 Mae S.H Wahyuningsih, Rul Afiyah Syarif, Zany sari Vindawati, Rita Rakhmawati
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 03 (2007)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Background: Tithonia diversifolia (Hemsley) A.Gray is a plant traditionally used to cure various diseases. The previous study indicated that n-hexane insoluble fraction of chloroform extract of T. diversifolia's leaves showed cytotoxic effect on Hela cells. Separation of n-hexane insoluble fraction into several fractions was conducted in order to simplify the components. Therefore, compounds responsible for cytotoxiceffect and their mechanism of action can be determined. .Objectives: To determine fraction having the most cytotoxic effect and its mechanism of action obtained from n-hexane insoluble fractions of T.diversifolia leaves on Hela cells.Materials and methods: Fractionation of n-hexane insoluble fraction from chloroform extract of T. diversifolia leaves was done by using vacuum liquid chromatography. By MTT assay, the cytotoxic effect of fractions on Hela cells in vitro was determined. and doxorubicin was used as positive control. Each group was tested for 3 times In = 3) and the growth inhibition percentage of Hela cells was counted. The data was analyzed by probit regression, and mechanism of action of the active fraction is conducted by staining with hoechst 33342.Results: Fractionation gave five fractions IF1-5). The ICso levels obtained of those fractions were : F1 having ICso = 32.168 :!: 12.513; F2 = 11.416 :!: 0.535; F3 III = 6.223 :!: 1.303; F4 = 94.308 :!: 7.843; and F5 = 41.740 :!: 6.381Ig/ml; while the ICso value of doxorubicin was 0.241 :!: 0.170 Iglml. Conclusions: Fraction 3 of the n-hexane insoluble displayed the highest cytotoxic effect on Hela cells with ICso= 6.223:!:: 1.303 Iglml. Fraction 3 at the concentration of 3.93 uglml caused chromatin condensation and fragmentation of DNA on Hela cells.Key words: T. diversifolia, cytotoxic effect. Hela cells, insoluble n-hexane fractions

Expression of CD5, CD1 0, Bcl-2 and Bcl-6 in diffuse large B cell lymphoma based on International Prognostic Index Indrawati Tody Guntersah
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 03 (2007)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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he correlation between HPV infection and p53, pRb, KI-67 as well as tunnel expression of cervical carcinoma Irianiwati Ahmad Ghozali Endang Soetristi
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 03 (2007)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Comparasion of Sport Urine Protein Creatinine From Three Different Time Urine Samples in Children With Primary Nephrotic Jusuf Hartanto Mohammad Juffrie Pungky Ardani
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 03 (2007)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Nurhadiyahya

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