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Jurnal Biologi Universitas Andalas
Published by Universitas Andalas
ISSN : -     EISSN : 23032162     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 4 Documents
 1 
Search results for , issue "Vol 10, No 1 (2022)" : 4 Documents clear
In Vitro Callus Induction in Tacca (Tacca chantrieri Andre) Leaf Explants on Murashige and Skoog Media with Different Concentrations of Sucrose Mayta Novaliza Isda; Melda Jannatul Salsabilla
Jurnal Biologi Universitas Andalas Vol 10, No 1 (2022)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.10.1.1-9.2022

Abstract

Tacca chantrieri belongs to the family Taccaceae has black flowers and has a long filiform that looks like a bat. T. chantrieri contains phytochemicals in the form of spritosol saponins used as traditional medicine by the people of China and Thailand. The amount of land clearing, forest exploitation and habitat destruction resulted in a reduction in the number of T. chantrieri, so T. chantrieri was propagated to maintain its sustainability. One way that can be used is the in vitro culture technique, namely callus culture. Callus culture is an early stage of in vitro culture technique where this stage aims to produce and multiply callus cells. The purpose of the study is were to determine the effect of different sucrose concentrations on callus induction from T. chantrieri leaf explants and determine the best sucrose concentrations for callus culture from T. chantrieri leaf explants on Murashige and Skoog (MS) media. This study used a single factor completely randomized design (CRD), namely sucrose concentrations 0 g/l, 10 g/l, 20 g/l, 30 g/l, 40 g/l, 50 g/l with five replications. The results of this study showed that the administrations of sucrose with different concentrations on MS media had an effect on increasing callus induction in Tacca chantrieri leaves. The best sucrose concertations for callus induction of Tacca chantrieri leaves was the addition of 40 g/l sucrose at 20 days after planting, 60 % callus formation percentage, callus formed in the form of compact callus and produce yellow-white callus. Keywords: Tacca chantrieri, sucrose, callus, Murashige and Skoog.
Ethnobotanical Study of Ferns as Traditional Medicine in Central Siberut, Mentawai Island Nova Syafni; Amri Bakhtiar
Jurnal Biologi Universitas Andalas Vol 10, No 1 (2022)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.10.1.10-14.2022

Abstract

West Sumatra Province has two ethnicities, namely Minangkabau and Mentawai. Both ethnicities have the capability to apply plants in folk medicine. This ethnobotanical study was conducted in Saibi Simokop village in three hamlets namely Sirisurak, Simoilaklak, and Saibi. The study was focused on the use of ferns in traditional medicine. Data collection was carried out using the snowball sampling method to seek the traditional healers (sikerei). The results showed that seven species of ferns were used for treatment of fever, inflammation, and poisoning. The ferns were included Cephalomanes javanicum (Blume) Bosch, Angiopteris evecta (G. Forst.) Hoffm., Dicranopteris linearis (Burm. F.) Underw., Diplazium esculentum (Retz.) Sw., Phymatosorus membranifolium (R. Br.) S.G. Lu, Lecanopteris sp., and Asplenium nidus L. The application of ferns in concoctions was administered topically and some were combined with oral administration.
Callus Regeneration of Gynura procumbens (Lour.) Merr. In Vitro Sitti Fatimah syahid; Lusia Seti
Jurnal Biologi Universitas Andalas Vol 10, No 1 (2022)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.10.1.15-22.2022

Abstract

Gynura procumbens (Lour.) Merr. is one of the species of Asteraceae which is potential as medicinal. Propagation of the species could be conducted by vegetative, so the plant genetic variability is narrow. Genetic variability could be increased by somaclonal variation through callus culture. There is no report on in vitro regeneration from callus culture, although this method could assist further genetic improvement of plants. In this experiment, different concentrations of 2,4- D (0.1 ; 0.3 ; 0.5 mg L -1 ) singly or combination with BA (0.1 ; 0.3 and 0.5 mg L -1 ) were evaluated for callus induction and several concentrations of BA (0 ; 0.1; 0.5 and 1.0 mg -1 ) combination with kinetin (0.1 and 0.3 mg-1) were observed for ability of callus formed shoots. The results showed that the best media for callus induction was 0.5 mg L -1 2,4-D + 0.5 mg L -1 BA. This treatment produced friable callus structure, no roots and yellowish white. Callus regeneration was obtained on the combination of 0.1 mg L -1 BA. + 0.1 mg L -1 kinetin and 0.1 mg L -1 BA + 0.5 mg L -1 kinetin but the percentage was still low. Keywords: G procumbens {Lour.} Merr, induction, regeneration, calli, in vitro
Antifungal Activity of Endophytic Bacteria isolated from Pegagan (Centella asiatica L.) for Inhibition the Growth of Malassezia furfur Vivi Yanthi; Mahyarudin Mahyarudin; Ambar Rialita
Jurnal Biologi Universitas Andalas Vol 10, No 1 (2022)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.10.1.23-32.2022

Abstract

Pityriasis versicolor is a superficial fungal infection characterized by changes in skin pigment due to stratum corneum colonization by Malassezia furfur. The Increasing of antifungal resistance and high recurrence rate, requires alternative treatment of medicinal plants. Pegagan (Centella asiatica L.) is known to produce secondary metabolites that have antifungal activity produced by endophytic bacteria that live on pegagan plant tissue. The study aimed to determine the antifungal activity of pegagan endophytic bacteria isolates to Inhibit the Growth of Malassezia furfur. The endophytic bacteria isolates were re cultured on NA media with a streak plate method. Antifungal activity test used the disk diffusion method which is characterized by the formation of inhibitory zones. Total of 37 endophytic bacteria isolates were successed re cultured, and there were 15 endophytic bacterial isolates that have potentcy as antifungal agent with inhibitory zones ranged from 6.5 to 15.52 mm. The most potential isolate was similarity with genus Pseudomonas. The secondary metabolites contained of alkaloids, terpenoids and saponins. The potential Centella asiatica endophytic bacteria had antifungal activity and similarity with the genus Pseudomonas.

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