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PENGELOMPOKAN POHON INDUK CENGKEH TERPILIH DI KABUPATEN SUMEDANG BERDASARKAN KARAKTER MORFOLOGI / Clustering of Progeny Clove Accessions from Cimanggu Population in Sumedang Based on the Morphologycal Characters Cheppy Syukur; nFN Sukarman; Nurliani Bermawie
Jurnal Penelitian Tanaman Industri Vol 22, No 1 (2016): Maret, 2016
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v22n1.2016.29-36

Cimanggu progeny clove has been distributed to almost of centre production, such as in Sumedang. However, their genotypes are still unidentified. Therefore, clustering cloves accession population in Sumedang is necessary. The objectives of this study is to cluster and determine phylogenetic relationship of Cimanggu progeny clove population in Sumedang base on morphological characters. The experiment was conducted in Sumedang (West, Java) from 2013 until 2014, by using 10 selected accessions (healthy, more than 40 years old, productivity is ≥ 20 kg dried flower). Variables were observed include morphological leaf, flower and flower production. To distinguish genotype from each accession was conducted by cluster analyzed, while to identify the variables which were related to the clusters formation, used correspondence analysis. The results indicated, that clove population Sumedang can be classified into three clusters as followed: the first cluster are Syar 43 and 46 accessions, characterized by greenish orange and greenish purple of young leaves tip with flower tube greenish red (GR 180B), the second cluster are Syar 44 and 45 accession, characterized by tip reddish purple young leaves and greenish red (GR181B) flower tubes color, and the third cluster are Syar 47, 49, 50, 51 and 52 accessions, characterized by orange young leaves tips and greenish red (GR180C) flower tubes. This information can be utilized to support releasing Cimanggu clove variety.Keywords: Syzygium aromaticum, clustering, characters, morphology

PENGARUH IRADIASI SINAR GAMMA (60Co) TERHADAP PERTUMBUHAN DAN PRODUKSI JAHE PUTIH KECIL (Zingiber officinale var.a ma rum) NURLIANI BERMAWIE; NUR LAELA W. MEILAWATI; S. PURWIYANTI; MELATI MELATI
Jurnal Penelitian Tanaman Industri Vol 21, No 2 (2015): Juni 2015
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v21n2.2015.47-56

ABSTRAKKeragaman genetik plasma nutfah jahe rendah sehingga perlu dilakukan peningkatan keragaman, antara lain dengan iradiasi sinar gamma. Penelitian ini bertujuan untuk mengetahui pengaruh dosis iradiasi terhadap pertumbuhan dan produksi jahe putih kecil. Penelitian dilakukan di Laboratorium Balai Penelitian Tanaman Rempah dan Obat serta Kebun Percobaan Cicurug, Sukabumi pada bulan Juni 2010 sampai dengan Mei 2011. Iradiasi dilakukan di P3TIR, BATAN, Jakarta. Percobaan disusun menggunakan rancangan split plot dengan petak utama dua aksesi jahe putih kecil ZIOF 0048 dan HALINA 1 dan anak petak adalah 11 dosis iradiasi (0-50 Gy) dengan interval 5 Gy. Perlakuan diulang sebanyak tiga kali. Pengamatan karakter morfologi dilakukan secara kuantitatif dan kualitatif pada umur 2, 3, dan 4 BST. Hasil penelitian menunjukkan aksesi berpengaruh terhadap radiosensitivitas. Aksesi HALINA 1 lebih sensitif dibandingkan dengan ZIOF 0048 ditunjukkan dengan nilai LD50 HALINA 1 (11,07 Gy) lebih rendah dari LD50 ZIOF 0048 (13,43 Gy). Interaksi aksesi pada dosis 5 dan 10 Gy memiliki nilai terbaik pada tinggi tanaman, ukuran daun serta jumlah anakan. Dosis mempengaruhi tinggi tanaman, ukuran daun, diameter dan panjang batang, serta jumlah daun. Pertumbuhan tanaman semakin meningkat dengan bertambahnya umur tanaman. Namun, semakin tinggi dosis iradiasi, pertumbuhan tanaman semakin melambat. Pertumbuhan tanaman terbaik diperoleh pada dosis 5 Gy, dan tidak berbeda dengan kontrol. Bobot dan ukuran rimpang terbaik (964 g/rumpun) diperoleh dari HALINA 1 pada dosis 10 Gy, dan berbeda dengan kontrol (454 g/rumpun), serta perlakuan lainnya.Kata kunci: Zingiber officinale var. amarum, iradiasi sinar gamma (60Co), keragaman genetik, mutasi, karakter morfologi. The Effect of Gamma Irradiation (60Co) on the Growth and Production of Small White Ginger (Zingiber officinale var. amarum)ABSTRACTGenetic variability of ginger germplasm was low, therefore it was necessary to broaden genetic variability using gamma irradiation. This research aimed to see the effect of irradiation on the growth of small white ginger. This study was conducted in the Plant Breeding Laboratory, Indonesian Spice and Medicinal Crops Research Institute and the Cicurug Experimental Garden Sukabumi from June 2010 to May 2011. Irradiation was undertaken in P3TIR, BATAN, Jakarta. The experiment was arranged in a split plot design with the main plot was two accessions HALINA 1 and ZIOF 00 48, and the sub plots was 11 irradiation doses (0-50 Gy) with 5 Gy interval. The experiment was replicated three times. Parameters observed were quantitative and qualitative characters at 2,3 and 4 months after planting (MAPs). Results showed that accession influenced radiosensitivity. Accession HALINA 1 was more radiosensitive than ZIOF 0048, indicated by LD50 of HALINA 1 was 11.0693 Gy, lower than ZIOF 0048 13.4254 Gy. Interaction between accessions at 5 and 10 Gy showed the highest plant height, leaf size, and number of shoots. Irradiation dose affected plant height, leaf size, stem diameter and length, and number of leaf. The plant growth increased in conjunction with plant age, but the higher irradiation dose, the growth of plants slowed down. The best plant growth was obtained from 5 Gy, not different from control. The highest rhizome weight (964 g/plant) was obtained from HALINA 1 treated with 10 Gy, differ from control (545 g/plant) and the other treatments.Keywords: Zingiber officinale var. amarum, gamma irradiation (60Co), genetic variability, mutation, morphological characters.

RESPON TANAMAN LADA (PIPER NIGRUML) VARIETAS CIINTEN TERHADAP IRADIASI SINAR GAMMA / Respons of Gamma Irradiation on Black Pepper (Piper nigrum L.) Ciinten Variety Nur Laela Wahyuni Meilawati; Nurliani Bermawie; Agus Purwito; Dyah Manohara
Jurnal Penelitian Tanaman Industri Vol 22, No 2 (2016): Juni, 2016
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v22n2.2016.71-80

Pepper is an introduced species and has always been propagated vegetatively, so it has narrow genetic base. High genetic diversity is necessary to produce new varieties, especially for breeding of resistance to foot rot disease. Increasing genetic diversity can be done through gamma ray irradiation. This research aims to evaluate response of black pepper Ciinten variety at seed and radicle emergence phases to gamma ray irradiation. The research was conducted in PAIR BATAN and greenhous e IMACRI from June 2014 to April 2015. The plant material was Ciinten variety at the seed and radicle emergence phases. The experimental design used was completely randomized design (CRD) with one factor which is dose of irradiation with seven levels (0, 25, 50, 75, 100, 125, 150) Gy. Each treatment consisted of three replications, each replication consisted of 60 seeds. Both phases showed significant differences in perfomances between dose in plant height, leaf length, number of leave, number of internode. Radiosensitivity of pepper on radicle emergence phase was higher than the seed phase indicated by LD50 (Lethal Dose 50). LD50 at seed phase was 68.15 Gy, whereas LD50 of the radicle emergence phase was 30 Gy. The higher irradiation dose that given to both treatment phases caused reduction in plant height, leaf length, while the number of leaves and nodes decreasing. Irradiation dose 25 dan 50 Gy in seed phase and 25 Gy in radicle emergence phase significantly increase genetic diversity base on quantitative, qualitative characters, anatomy and leaf resistence to P. capsici infection. Eighteen putative mutants resistant to infection P. capsici. Eighteen mutant putative Ciinten pepper varieties were expected to be high yielding varieties with more research in the greenhouse and in the field to determine the production and quality potential.

PENGARUH PENGENCERAN MEDIA DASAR TERHADAP PERTUMBUHAN KULTUR JAHE DALAM PENYIMPANAN SECARA IN VITRO SITTI FATIMAH SYAHID; NURLIANI BERMAWIE
Jurnal Penelitian Tanaman Industri Vol 5, No 4 (2000): Maret, 2000
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v5n4.2000.115-118

Effect of dilution of basic medium on ginger growth during conservation in vitroBasic medium dilution could affect the growth of the culture. The effect of basic medium dilution was evaluated at the laboratory of Research Institute for Spices and Medicinal Crops from October 1998 to June 1999. Sterile shoots of ginger were used as ex plants. Basic medium dilution from 3/4 MS, 1/2 MS, 1/4 MS and MS normal (control) were used as treatments. The media were enriched with BA 3 mg/1 and Sucrose 6%. The treatments wee arranged in a completely randomized design with three eplications. Results showed that all the dilution treatments inhibited the grow of the culture during live months of conservation period, especially on 1/4 MS + BA 3 mg/1 + Sucrose 6% . On the media, the number of shoots wee 3.55. Based on cultue performance the best medium for conservation was 1/2 MS + BA 3 mg/1 + Sucrose 6%. All of the culture was able to growth in regeneration media after they were conserved for ive months. Results of this experiment could be applied in reducing sub-cultue frequency to a new media and maintain the culture stability. e

KONSERVASI TANAMAN LADA (Piper nigrum L.) SECARA IN VITRO YELNITITIS YELNITITIS; NURLIANI BERMAWIE
Jurnal Penelitian Tanaman Industri Vol 7, No 3 (2001): September, 2001
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v7n3.2001.88-92

In vitro conservation of black pepper (Piper nigrum L.)Black pepper (Piper nigrum L.) is one of the economically im¬ potant spices. The major constraint in black pepper cultivation and conservation in ield is foot rot disease caused by Phytopthora capsici which could cause plants die. Conservation of black pepper germplasms as living collections in ield is risky due to pests and natural disaster. The experiment on in vitro cop ervation of black pepper var. LDL was conducted al the laboratory of Plant Genetic Resources and Breeding, Research Institute for Spice and Medicinal Crops (RISMC) Bogor from April 1998 to Maret 1999. Single node cuttings from sterile culture were used as explains. The explains were cultured on Murashige and Skoog (MS) medium on full and half strength concentration supplemented with paclobutrazol (paclo) (0, 1, 3 and 5 mg/1). The experiment was performed in a randomized complete block design arranged factorially with 10 replications. The result showed that the medium supplemented with paclo on both full MS and MS A medium could suppress vegetative growth until 12 months. There was no signiicant interaction between medium and paclo on shoot initiation. The effect was signiicant on shoot height, number of leaves and culture performances. Increasing paclo concen¬ tration caused higher suppression of plant growth. MS A medium supplemented with paclo 5 mg/1 showed the slowest growth with shool height 2.10 cm and number of leaves 9. Culture performance was fresh, with green leaves and vigorous. Advcntive shoots were able to regenerate on the medium supplemented with BA 0.3 mg/1. In vitro conservation of black pepper with paclo did not change plant regeneration ability. Therefore, this technique may be used as an altenative method for black pepper conservation.

PENGARUH SUBKULTUR DAN LAMA PERIODE KULTUR PADA DAYA MULTIPLIKASI TUNAS LADA (Piper nigrum L.) ASAL BIJI VARIETAS PETALING I NATALINI NOVA KRISTINA; NURLIANI BERMAWIE
Jurnal Penelitian Tanaman Industri Vol 5, No 3 (1999): Desember, 1999
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v5n3.1999.98-102

Effect subculture and culture period on shoot multipli¬ cation in black pepper Petaling IAn expeiment on shoot multiplication in black pepper Petaling I from planting to until shoot multiplication changes and the effect of culture peiod, was conducted in the Laboratory of Genetic Research and Breeding of Balittro. Bogor from May 1997 to June 1998. The seeds wee germinated on MS medium without growth regulator. Ater sprouting the shoots wee removed to MS multiplication medium + BA 0.3 mg/1 + polypynyl pyolidon 200 mg/1 for subcultue and culture peiod. In the subculture expeiment, explant was removed after 3 months to new medium. In the cultue peiod the explant was visible until 4 months after that, explant was removed to new medium. The results showed that the highest multiplication rate was found after 3'° subculture with 9.2 shoots and the best cultue peiod was three months with 9.3 shoots. After 4* months the shoots died, due to fenolic activity which changed pH medium from acidic (5.8 to basic 7.13).

OBSERVASI HASIL DAN MUTU LADA LOKAL DI DUA AGROEKOLOGI Nurliani Bermawie; Sri Wahyuni; Ruby Heryanto; Rudi T Setiyono; Laba Udarno
Buletin Penelitian Tanaman Rempah dan Obat Vol 24, No 2 (2013): Buletin Penelitian Tanaman Rempah dan Obat
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v24n2.2013.%p

Upaya peningkatan produktivitas lada dapat dilakukan dengan penggunaan bahan tanaman unggul lokal. Tujuan dari penelitian ini adalah untuk mengetahui potensi hasil, komponen hasil dan mutu lada lokal asal Sukabumi dan varietas pembanding Petaling-1, di dua agroekologi berbeda, di kabupaten Sukabumi dan kabupaten Purwakarta. Pengamatan dilakukan pada 10% tanaman contoh dari tiap populasi, pada sifat hasil per pohon, komponen hasil dan mutu. Data dianalisis dengan uji t. Pertumbuhan, hasil, komponen hasil dan mutu lada kedua varietas secara umum lebih baik di Purwakarta dari pada di Sukabumi. Hasil uji t memperlihatkan bahwa hasil per pohon, karakter jumlah malai per tanaman, panjang malai, jumlah biji per malai, bobot malai dan panjang tangkai malai berbeda antara varietas lokal dengan varietas Petaling-1, dan lada lokal lebih baik dari Petaling-1. Hasil lada lokal per pohon di Purwakarta 2,79 kg, dan varietas Petaling-1, yaitu1,67 kg per pohon. Di Sukabumi hasil buah segar lada lokal juga lebih tinggi dari varietas Petaling-1 berturut-turut 2,13 kg dan 1,30 kg per pohon. Malai lada lokal 9-13,5 cm, dengan jumlah buah per malai 70-140 butir, lebih panjang dan lebih banyak dari malai Petaling-1 berturut-turut 5-9 cm, dan 20-60 butir. Mutu lada putih maupun lada hitam berbeda antar lokasi, dan mutu di Purwakarta lebih baik dari pada di Sukabumi. Di kedua lokasi, mutu lada lokal baik kadar minyak atsiri, oleoresin maupun piperin lebih baik dari Petaling-1. Bobot buah segar lada lokal sama dengan Petaling-1 sehingga cocok untuk diproses menjadi lada putih.

PENGARUH SUBKULTUR DAN LAMA PERIODE KULTUR PADA DAYA MULTIPLIKASI TUNAS LADA (Piper nigrum L.) ASAL BIJI VARIETAS PETALING I NATALINI NOVA KRISTINA; NURLIANI BERMAWIE
Jurnal Penelitian Tanaman Industri Vol 5, No 3 (1999): Desember, 1999
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Effect subculture and culture period on shoot multipli¬ cation in black pepper Petaling IAn expeiment on shoot multiplication in black pepper Petaling I from planting to until shoot multiplication changes and the effect of culture peiod, was conducted in the Laboratory of Genetic Research and Breeding of Balittro. Bogor from May 1997 to June 1998. The seeds wee germinated on MS medium without growth regulator. Ater sprouting the shoots wee removed to MS multiplication medium + BA 0.3 mg/1 + polypynyl pyolidon 200 mg/1 for subcultue and culture peiod. In the subculture expeiment, explant was removed after 3 months to new medium. In the cultue peiod the explant was visible until 4 months after that, explant was removed to new medium. The results showed that the highest multiplication rate was found after 3'° subculture with 9.2 shoots and the best cultue peiod was three months with 9.3 shoots. After 4* months the shoots died, due to fenolic activity which changed pH medium from acidic (5.8 to basic 7.13).

PENGELOMPOKAN POHON INDUK CENGKEH TERPILIH DI KABUPATEN SUMEDANG BERDASARKAN KARAKTER MORFOLOGI / Clustering of Progeny Clove Accessions from Cimanggu Population in Sumedang Based on the Morphologycal Characters Cheppy Syukur; nFN Sukarman; Nurliani Bermawie
Jurnal Penelitian Tanaman Industri Vol 22, No 1 (2016): Maret, 2016
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v22n1.2016.29-36

Cimanggu progeny clove has been distributed to almost of centre production, such as in Sumedang. However, their genotypes are still unidentified. Therefore, clustering cloves accession population in Sumedang is necessary. The objectives of this study is to cluster and determine phylogenetic relationship of Cimanggu progeny clove population in Sumedang base on morphological characters. The experiment was conducted in Sumedang (West, Java) from 2013 until 2014, by using 10 selected accessions (healthy, more than 40 years old, productivity is ≥ 20 kg dried flower). Variables were observed include morphological leaf, flower and flower production. To distinguish genotype from each accession was conducted by cluster analyzed, while to identify the variables which were related to the clusters formation, used correspondence analysis. The results indicated, that clove population Sumedang can be classified into three clusters as followed: the first cluster are Syar 43 and 46 accessions, characterized by greenish orange and greenish purple of young leaves tip with flower tube greenish red (GR 180B), the second cluster are Syar 44 and 45 accession, characterized by tip reddish purple young leaves and greenish red (GR181B) flower tubes color, and the third cluster are Syar 47, 49, 50, 51 and 52 accessions, characterized by orange young leaves tips and greenish red (GR180C) flower tubes. This information can be utilized to support releasing Cimanggu clove variety.Keywords: Syzygium aromaticum, clustering, characters, morphology

PENGARUH IRADIASI SINAR GAMMA (60Co) TERHADAP PERTUMBUHAN DAN PRODUKSI JAHE PUTIH KECIL (Zingiber officinale var.a ma rum) NURLIANI BERMAWIE; NUR LAELA W. MEILAWATI; S. PURWIYANTI; MELATI MELATI
Jurnal Penelitian Tanaman Industri Vol 21, No 2 (2015): Juni 2015
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v21n2.2015.47-56

ABSTRAKKeragaman genetik plasma nutfah jahe rendah sehingga perlu dilakukan peningkatan keragaman, antara lain dengan iradiasi sinar gamma. Penelitian ini bertujuan untuk mengetahui pengaruh dosis iradiasi terhadap pertumbuhan dan produksi jahe putih kecil. Penelitian dilakukan di Laboratorium Balai Penelitian Tanaman Rempah dan Obat serta Kebun Percobaan Cicurug, Sukabumi pada bulan Juni 2010 sampai dengan Mei 2011. Iradiasi dilakukan di P3TIR, BATAN, Jakarta. Percobaan disusun menggunakan rancangan split plot dengan petak utama dua aksesi jahe putih kecil ZIOF 0048 dan HALINA 1 dan anak petak adalah 11 dosis iradiasi (0-50 Gy) dengan interval 5 Gy. Perlakuan diulang sebanyak tiga kali. Pengamatan karakter morfologi dilakukan secara kuantitatif dan kualitatif pada umur 2, 3, dan 4 BST. Hasil penelitian menunjukkan aksesi berpengaruh terhadap radiosensitivitas. Aksesi HALINA 1 lebih sensitif dibandingkan dengan ZIOF 0048 ditunjukkan dengan nilai LD50 HALINA 1 (11,07 Gy) lebih rendah dari LD50 ZIOF 0048 (13,43 Gy). Interaksi aksesi pada dosis 5 dan 10 Gy memiliki nilai terbaik pada tinggi tanaman, ukuran daun serta jumlah anakan. Dosis mempengaruhi tinggi tanaman, ukuran daun, diameter dan panjang batang, serta jumlah daun. Pertumbuhan tanaman semakin meningkat dengan bertambahnya umur tanaman. Namun, semakin tinggi dosis iradiasi, pertumbuhan tanaman semakin melambat. Pertumbuhan tanaman terbaik diperoleh pada dosis 5 Gy, dan tidak berbeda dengan kontrol. Bobot dan ukuran rimpang terbaik (964 g/rumpun) diperoleh dari HALINA 1 pada dosis 10 Gy, dan berbeda dengan kontrol (454 g/rumpun), serta perlakuan lainnya.Kata kunci: Zingiber officinale var. amarum, iradiasi sinar gamma (60Co), keragaman genetik, mutasi, karakter morfologi. The Effect of Gamma Irradiation (60Co) on the Growth and Production of Small White Ginger (Zingiber officinale var. amarum)ABSTRACTGenetic variability of ginger germplasm was low, therefore it was necessary to broaden genetic variability using gamma irradiation. This research aimed to see the effect of irradiation on the growth of small white ginger. This study was conducted in the Plant Breeding Laboratory, Indonesian Spice and Medicinal Crops Research Institute and the Cicurug Experimental Garden Sukabumi from June 2010 to May 2011. Irradiation was undertaken in P3TIR, BATAN, Jakarta. The experiment was arranged in a split plot design with the main plot was two accessions HALINA 1 and ZIOF 00 48, and the sub plots was 11 irradiation doses (0-50 Gy) with 5 Gy interval. The experiment was replicated three times. Parameters observed were quantitative and qualitative characters at 2,3 and 4 months after planting (MAPs). Results showed that accession influenced radiosensitivity. Accession HALINA 1 was more radiosensitive than ZIOF 0048, indicated by LD50 of HALINA 1 was 11.0693 Gy, lower than ZIOF 0048 13.4254 Gy. Interaction between accessions at 5 and 10 Gy showed the highest plant height, leaf size, and number of shoots. Irradiation dose affected plant height, leaf size, stem diameter and length, and number of leaf. The plant growth increased in conjunction with plant age, but the higher irradiation dose, the growth of plants slowed down. The best plant growth was obtained from 5 Gy, not different from control. The highest rhizome weight (964 g/plant) was obtained from HALINA 1 treated with 10 Gy, differ from control (545 g/plant) and the other treatments.Keywords: Zingiber officinale var. amarum, gamma irradiation (60Co), genetic variability, mutation, morphological characters.

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