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EFFECTS OF NUTRIENT CONCENTRATION IN MULTI STAGE FEEDING OF THE GROWTH OF MICROALGAE Nannochloropsis sp. Sriwuryandari, Lies; Priantoro, Ekaputra Agung; Sembiring, Tarzan
Teknologi Indonesia Vol 40, No 2 (2017)
Publisher : LIPI Press

Renewable energy plays an important role for a sustainable future and the use of microalgae Nannochloropsis sp., as a potential source of biofuel due to its high lipid content, makes a good option. The nutrient found in the growth medium of microalgae have been found to affect cell synthesis, lipid synthesis, as well as its fatty acid profile. This study was conducted to observe the effects of the N-P concentration utilizing multistage feeding on the performance of Nannochloropsis sp. Sodium bicarbonate, urea, and sodium dihydrogen phosphate monohydrate were used as sources of C, N, and P for the microalgae. The nutrient feeding needed was calculated based on a molecular formula of microalgae(CO0.48H1.83N0.11P0.01) with a production target 1g.L-1 cell biomass. Element C is an iso-molar. The concentration of N- P varies: corresponding to the mol-fraction of the microalgae cell (as a control) and 10% excess. There were four levels of treatment: A (U0P0); B (U10P0); C (U0P10); and D (U10P10) with three replicants in the batch culture system. Nutrient feeding was conducted in three stages: I (40%); II (30%);and III (30%) and incubated in a neutral environment. Nannochloropsis sp. was able to grow well at a temperature of 24–35°C and an acidity level (pH) of 9.0–10.5. The culture (on the 21st day) produced DCW 0.93; 1.04; 0.86;1.12g.L-1, Chlorophyll-a 7.08; 7.62; 4.64; 6.85% DC, lipid 19.2; 15.5; 27.3; and 21.6% DC for treatments A; B;C; and D, respectively.The maximum lipid level resulted in an N deficiency and a P surplus (C). The lipids of the Nannochloropsis sp. composed of 66.0–81.1% saturated fatty acid which is suitable to be used as a biofuel.

LIQUID BIOFERTILIZER AND COMPOST FROM ORGANIC MARKET WASTE Sriwuryandari, Lies; Sembiring, Tarzan
Teknologi Indonesia Vol 33, No 2 (2010)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jti.v33i2.22

Liquid biofertilizer and compost from organic market waste were produce by the addition of some functional microorganisms. Azospirrillium sp., Azotobacter sp., Lactobacillus sp., Pseudomonas sp. and Actinomycetes were inoculated into liquid or solid raw materials. The ripe biofertilizer were characterized and meets the Indonesian National standards for biofertilizer. Actinomycetes contained in the liquid fertilizer about 2.95 x 108 cfu/ml, which is meet the standard (? 104 cfu/ml) and other functional microorganisms namely Lactobacillus sp., Azospirillium sp., Azotobacter sp., Pseudomonas sp. and phosphate diluters were grown enormously with the population of more than 105 cfu/ml respectively. Qualitative test of the fertilizer shown that the fertilizer are effective for the crops such as Long bean (Vigna unguiculata), Cucumber (Cucumis sativus), Leek (Allium ampeloprassum) and others. Production of biofertilizer using organic market waste support the organic farming and the national policy on the fertilizer.

Propionic Acid Metabolism During Anaerobic Biowaste Slurry Digestion Sembiring, Tarzan; Ekoputranto, Agung; Sriwuryandary, Lies; Nilawati, Dewi; Gallert, C; Winter, J
Teknologi Indonesia Vol 35, No 1 (2012)
Publisher : LIPI Press

Solid waste was found to be a problem in the city sanitary and river management in Indonesia. Bio-waste from traditional market, which is as the major part of the solid waste of the city, is one of the big problem in the sanitary management. To over come of the problem of the bio-waste, the experiment on the anaerobic biodegradation of organic waste slurry in stirred tank bioreactor was executed. The substrate was feeding into reactor and inoculated with an anaerobic enriched mixed culture originally from cow intestinal micro flora. The total solid of substrate was around 10 percent. During the batch experiment showed that the pH value relatively stable around pH 7 but the pH is decreased in the end of the experiment. The degradation of organic slurry is shown by the decreasing of COD content from around 90 g/l to 20 g/l and the formation of volatile fatty acids. Methane content of the biogas produce is more than 60 %. The volatile fatty acids detected are acetic acid, propionic acid, butyric acid and valeric acid. The fluctuation of volatile fatty acids formation and its depletions were relatively good as well as the depletion of Chemical Oxygen Demands, in which the pH value is around 7.5 Propionic acid and butyric acid degrading bacteria were also found in the consortium of mixed culture as indicated by the elimination of those volatile fatty acids during the experiment and in the enrichment cultures. Observation of the cultures of propionic acid degrading enrichment under the microscope shown that the morphology of the bacteria were rod with dimension of 0.81 ?m x 2-3 ?m ( d x l ) and the better gown in pH 6.57.

Biodegradasi Limbah Padat Organik secara anaerob: Proses semi kontinu Sriwuryandari, Lies; Sembiring, Tarzan
Teknologi Indonesia Vol 32, No 2 (2009)
Publisher : LIPI Press

On the minimizing of organic solid waste coming to open dumping area, the municipal organic waste was used as substrate for anaerobic degradation and methane generation. Experiment either in the semi continuous bioreactor or in serum vials showed that the rate of organic substance conversion is low. The degradation rate is between 3550 % of organic material feed into bioreactor where pH is in optimum condition for methane generation. The overall limiting step of organic material biodegradation is the hydrolysis of organic fiber into soluble organic substance.

GROWTH RESPONSE OF NANNOCHLOROPSIS AT OUTDOOR CULTIVATION IN MEDIUM WITH VARIATION OF NITROGEN CONCENTRATION Astuti, J. Tri; Sriwuryandari, Lies; Yusiasih, Retno; Sembiring, Tarzan
Teknologi Indonesia Vol 33, No 2 (2010)
Publisher : LIPI Press

Micro-algae are fast-growing unicellular photosynthetic microorganisms that able to fi x CO2 while capturing solar energy. Source and concentration of nitrogen itself affected to the growth character and composition of microalgae. Cultivation with limited of nitrogen resulted great increase in the lipid content. This study was conducted to investigate the infl uence of nitrogen concentration to the growth rate and lipid characteristics of Nannochloropsis sp. In this study, ammonium nitrate (NH4NO3) was added in different concentration, i.e. 0; 30; 70; and 100% based on nitrogen content in f/2 medium, or equivalent to 0; 1.06; 2.47; and 3.53 (10-4 M). All treatment was prepared triplicate, then cultivated at outdoor batch culture systems and aerated continuously with a sterilized of atmospheric air. The algae biomass was harvested at 14th day after inoculated and then analyzed. Data showed that Nannochloropsis sp. could be cultivated in the outdoor batch system, which temperature was in the range of 26.1137.97oC, with average of 33.44oC. Compared to its initial condition, pH culture increased signifi cantly from 6.92 to 8.54. The highest OD680nm (0.500) and dry cell concentration (458mg.L-1) was obtained by using NH4NO3 at 2.47x10-4 M. The lipid content was 63.58% and 46.59% of dry cell weight when using nitrogen 1.06x 10-4M and 2.47x10-4M, respectively. Composition of fatty acid was not signifi cantly affected by the changing of nitrogen concentration. Palmitic acid (C16:0) was the most of fatty acid in Nannochloropsis sp. (44.4753.97%), followed by lauric acid (25.7834.34%), and myrictic acid (19.0721.19%). The using of N-NH4NO3 at 2.47x10-4M gave the highest lipid content of 213.15mg.L-1.

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