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All Journal AcTion: Aceh Nutrition Journal Dharma Bakti Jurnal Kesehatan SANITAS: Jurnal Teknologi dan Seni Kesehatan Medical Sains : Jurnal Ilmiah Kefarmasian Medical Sains : Jurnal Ilmiah Kefarmasian
Rachmawati, Siti Rahayu
Poltekkes Kemenkes Jakarta II
Humanities Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Other

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Journal : SANITAS: Jurnal Teknologi dan Seni Kesehatan

 1 
Characterization Of Moringa (Moringa Oleifera Lam.) Leaf Water Extracts By Chemical And Microbiology Siti Rahayu Rachmawati; junie Suriawati
SANITAS: Jurnal Teknologi dan Seni Kesehatan Vol 10 No 2 (2019): SANITAS Volume 10 Nomor 2 Tahun 2019
Publisher : Politeknik Kesehatan Kemenkes Jakarta II

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36525/sanitas.2019.11

Abstract

Moringa leaves or referred to Moringa oleifera Lam. are belong to the Moringaceae tribe. This plant is reported to have antibacterial activity and high nutritional value. To be used as an active ingredient in food preparations, it needs to be made in the form of extracts. The study aims to determine the characteristics of Moringa leaf water extract. In this study, Moringa leaves were extracted with a water solvent. Chemical characterization of Moringa leaf extract includes qualitative phytochemical tests and nutritional values ​​(water content, ash, protein, fat, and carbohydrate). Whereas in microbiology, they include microbial contamination (Total Plate Count and Total Yeast and Mold Count). The results of characterization testing of the extracts showed that the water extract of Moringa leaves contain active compounds: flavonoids, phenols, triterpenoids/steroids, saponins, and tannins. Microbial contamination contained in the water extract of Moringa leaves are Total Plate Count value of 0 colonies/g and Mold/Yeast count value of 0 colonies/g and contain nutritional values such as water (75.85%), ash (3.87%), protein (6.27%), fat (<2.20 %) and carbohydrate (14.01%).
Antibacterial Activities Test of Combination of Ethanolic Extract of Betel Leaves (Piper betle L.) and Basil Leaves (Ocimum basilicum L.) Against Staphylococcus aureus Junie Suriawati; Patimah Patimah; Siti Rahayu Rachmawati
SANITAS: Jurnal Teknologi dan Seni Kesehatan Vol 9 No 2 (2018): SANITAS Volume 9 Nomor 2 Tahun 2018
Publisher : Politeknik Kesehatan Kemenkes Jakarta II

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36525/sanitas.2018.13

Abstract

Betel leaf extract and basil leaf extract contain phenol compounds, safonin, flavonoids, tannins, and essential oils that act as antibacterials. Skin is very susceptible to infection or other skin diseases one of which is caused by Staphylococcus aureus bacteria. This research needs to be done to test the antibacterial activity of ethanol extract of green betel leaf, basil leaf and combination of both extracts to S. aureus. The process of extraction of betel leaf and basil leaves was done with 96% ethanol solvent using maceration method. The combinations used in the sample solution were the concentration of ethanol extract of green betel leaf (0%, 15%, 30%, 45%, 60%, 75%) and basil leaf extract (0%, 15%, 30%, 45%, 60 %, 75%). Furthermore, the antibacterial activity was tested for S. aureus with well diffusion method. The data obtained were tested statistically using Anova and Duncan's advanced test. The result of statistical analysis showed difference of drag zone (p <0,05) between various concentration of ethanol extract of green betel leaf, basil leaf and combination of both extracts to S. aureus. The smallest resin zone of ethanol extract of betel leaf and ethanol extract of basil leaves is at 0% concentration (S0 and K0) and highest at 75% concentration (S5 and K5). While the smallest zone of inhibition for the combination of ethanol extract of betel leaf and ethanol extract of basil leaves at concentration S0K0, S0K1 and S0K2 is 0 mm and highest at S5K5 concentration is 31,3 mm. The higher concentration of betel leaf extract, basil leaf extract and the combination of the two extracts, the inhibition of diameter zone will be better. It can be concluded that the combination of betel leaf extract and basil leaf extract has the ability to inhibit the growth of S. aureus better than single extract.
Sterility Test of Syringes As A Pharmaceutical Preparation That Obtained From Pasar Pramuka Reni Anggraini; Junie Suriawati; Siti Rahayu Rachmawati; Yulis Adriana
SANITAS: Jurnal Teknologi dan Seni Kesehatan Vol 12 No 2 (2021): SANITAS Volume 12 Nomor 2 Tahun 2021
Publisher : Politeknik Kesehatan Kemenkes Jakarta II

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36525/sanitas.2021.17

Abstract

Syringes are one of the pharmaceutical preparations that are in high demand. In healthcare institutions, syringes are used to aid in patient care and examination. Pharmaceutical preparations, such as various syringes, are widely available in the Pasar Pramuka. Syringes must be free from microbes and used syringes should not be reused. Microbiological sterility tests can be performed on a syringe to determine whether it is sterile or not. The purpose of the study is to test the sterility of syringes obtained from the Pasar Pramuka. A random sample of a syringe is being used in the study as an experimental method. The syringes were isolated and incubated for 14 days in Fluid Thioglycollate Medium (FTM) and Trypticase Soy Broth (TSB) at 30-35 oC and 20-25 oC, respectively, with frequent observations. If FTM and TSB media were turbid, then isolated into selective media based on their microbe as controls, namely Clostridium sporogenes ATCC 19404, Pseudomonas aeruginosa ATCC 9027, Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 25922, Escherichia coli ATCC 8739, Bacillus subtilis ATCC 6633, Salmonella typhi. The results showed that the B syringe was turbid on FTM media and did not contain pathogenic microbes after being identified on selective media, as in controls. The A and C syringes on TSB media were turbid, and after identification on selective media, Candida albicans and Aspergillus brasiliensis were found. In conclusion, the A, B, and C syringes are not steril.
Co-Authors Junie Suriawati Latirah Lisawati Tanzil Patimah Patimah Reni Anggraini Suzana Indah Astuti Yulis Adriana