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All Journal JURNAL FARMASI GALENIKA Indonesian Journal of Pharmaceutical and Clinical Research
Permata, Yade Metri
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Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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ANALISIS KADAR KALIUM DAN DAYA LARUT KALSIUM OKSALAT OLEH INFUSA SELADA (Lactuca sativa L.) SECARA SPEKTROFOTOMETRI SERAPAN ATOM Permata, Yade Metri; Angkat, Lisbeth; Wahyuni, Henny Sri
JURNAL FARMASI GALENIKA Vol 4 No 2 (2017): Jurnal Farmasi Galenika Volume 4 No. 2, 2017
Publisher : Sekolah Tinggi Farmasi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (375.213 KB)

Abstract

Obat-obatan dari bahan tumbuhan sudah menjadi bagian dari kehidupan sehari-hari masyarakat Indonesia. Selada (Lactuca sativa L.) merupakan sayuran yang sudah lama dikonsumsi oleh masyarakat karena mempunyai penampilan yang menarik dengan warna hijau yang segar, dapat digunakan sebagai lalapan, dan mempunyai nilai tambah terhadap kesehatan. Selada mempunyai kandungan gizi yang tinggi terutama mineralnya seperti kalium, natrium, magnesium, kalsium, fosfor, dan zat besi, vitamin A, vitamin B, dan vitamin C. Tumbuhan yang mempunyai kandungan kalium yang tinggi mempunyai daya larut yang baik terhadap kalsium oksalat, sehingga tujuan penelitian ini adalah untuk mengetahui kandungan kalium dan kemampuan daya larutnya terhadap kalsium oksalat. Daun selada dibuat dalam sediaan infusa dengan prosedur menurut Farmakope Indonesia edisi IV dan kelarutan kalsium oksalat di dalam infusa dilakukan dengan inkubasi selama 4 jam, selanjutnya kadar kalium dan kalsium sebelum dan sesudah inkubasi pada infusa dianalisis secara kuantitatif dengan metode spektrofotometri serapan atom. Hasil analisis kalium dan kalsium pada infusa selada sebelum inkubasi adalah (486,2133±7,8057) μg/ml dan (152,0783±4,0871) μg/ml, sedangkan kadar setelah inkubasi adalah (492,4833±3,5438) μg/ml dan (192,2367±4,0249) μg/ml, dengan konsentrasi ion kalsium terlarut sebesar (4,0158±0,5641) mg/100ml.
Total Phenolic Content and Antibacterial Activity of Nut Grass (Cyperus rotundus L.) Extract Masfria; Metri Permata, Yade
Indonesian Journal of Pharmaceutical and Clinical Research Vol. 1 No. 1 (2018): Indonesian Journal of Pharmaceutical and Clinical Research
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (864.021 KB) | DOI: 10.32734/idjpcr.v1i1.202

Abstract

Abstract. The objectives of this research was to study the macroscopic, the microscopic and phytochemicals characterization of raw materials, total polyphenol content and the antibacterial activity of nut grass extracts (Cyperus rotundus L.) against Shigella dysenteriae, Salmonella typhimurium, Staphylococcus epidermidis, and Pseudomonas aeroginosa. The macroscopic and the microscopic characterization of raw materials were done with the determination of the water contents, the water soluble contents, the ethanol soluble contents, total ash and acid soluble ash. The extract was prepared by percolation using n-hexane, ethanol, and ethyl acetate as solvents. Phytochemical screening was done on the raw materials as well as n-hexane, ethyl acetate, and ethanol extracts. Total phenol contents were tested with the Folin-Ciocalteu reagent method. The antibacterial activity test was done by measuring the diameter of the inhibition zones using the diffusion agar method. The results showed that raw material retrieved 7.33% water content, 21.85% water soluble contents, 7.9% ethanol soluble contents, 1.53% total ash, and 0.67% total insoluble-acid ash. The phytochemical study showed that the nut grass contains various secondary metabolites including flavonoids, glycosides, saponins, anthraquinone glycosides, and steroid/triterpenoids. The total polyphenol contents of ethanol extracts were expressed as catechin equivalents 1.1616 mg/g extract. All of the nut grass extract showed antibacterial activity. Ethyl acetate extract of nut grass indicated the highest antibacterial activity against Shigella dysenteriae, Salmonella typhimurium, and Pseudomonas aeroginosa. Ethanol extract was only effective against Staphylococcus epidermidis. Keywords: Antibacterial Activity, Bacteria, Cyperus Rotundus L Extracts, Phenolic
Co-Authors Anggi Tri Liesna Angkat, Lisbeth Fahmi Muhammad Rangkuti Henny Sri Wahyuni Kharisma Insyra Masfria Yasmin Dayanti