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All Journal The Indonesian Biomedical Journal Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia
Moehario, Lucky Hartati
Department Of Microbiology, Faculty Of Medicine And Health Science, Atma Jaya Catholic University Of Indonesia, Jl. Pluit Raya No. 2, Jakarta
Biochemistry, Genetics & Molecular Biology Education Health Professions Medicine & Pharmacology Public Health

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DETEKSI ANTIGEN HELICOBACTER PYLORI PADA PASIEN GERD DAN NON-GERD DI RUMAH SAKIT ATMA JAYA Athalia Nofera Kurniawan; Riki Tenggara; Lucky Hartati Moehario
Majalah Kedokteran Indonesia Vol 69 No 8 (2019): Journal of the Indonesian Medical Association Majalah Kedokteran Indonesia Volum
Publisher : PENGURUS BESAR IKATAN DOKTER INDONESIA (PB IDI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/jinma.v69i8.185

Abstract

Pendahuluan: Helicobacter pylori adalah salah satu bakteri flora normal lambung dan sering diasosiasikan dengan dispepsia, tetapi keterkaitannya terhadap Gastroesophageal Reflux Disease (GERD) masih kontroversial. Diagnosis H. pylori paling sering ditegakkan melalui pemeriksaan histopatologi, tes urease yang invasif, dan urea breath test (UBT) yang relatif mahal. Salah satu uji serologi adalah stool antigen test (SAT) yang lebih murah, non-invasif dan lebih mudah pengerjannya. Penelitian ini bertujuan mendeteksi adanya antigen H. pylori pada pasien GERD dan non-GERD di Rumah Sakit Atma Jaya (RSAJ). Metode: Penelitian ini menggunakan studi potong lintang yang bersifat deskriptif. Inklusi penelitian mencakup pasien dewasa yang dirawat inap pada bulan Agustus 2018 ? November 2018 di RSAJ. Peserta diwawancara dengan kuesioner GERDQ. Sampel feses diperiksa menggunakan SAT (Biocare®). Hasil: Sebanyak 30 responden yang terdiri 22 perempuan dan 8 laki-laki, dengan usia terbanyak adalah kelompok usia dewasa (35?64 tahun). Sebanyak 40% responden termasuk kelompok GERD dengan 75% perempuan. Hasil uji SAT positif pada kelompok GERD 16,67%, non-GERD 11,11% dan hasil uji SAT negatif pada kelompok GERD 91,67%, non-GERD 77,78%. Kesimpulan: Keberadaan antigen H. pylori lebih banyak ditemukan pada perempuan, pada usia dewasa dan tidak ditemukan perbedaan antigen H. pylori pada pasien GERD dan non-GERD di RS Atma Jaya.
Gene Families of AmpC-producing Enterobacteriaceae Present in the Intensive Care Unit of Cipto Mangunkusumo Hospital Jakarta Lucky Hartati Moehario; Thomas Robertus; Anis Karuniawati; Rudyanto Sedono; Delly Chipta Lestari; Andi Yasmon
The Indonesian Biomedical Journal Vol 11, No 1 (2019)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v11i1.552

Abstract

BACKGROUND: Antibiotic resistance has become a worldwide problem. Among Asia countries, Indonesia has high prevalence of multi-drug resistant organisms mainly due to Gram-negative bacilli Enterobacteriaceae. This study aimed to find out whether gene family of AmpC and AmpC/ESBL-producing Enterobacteriaceae were present in the Intensive Care Unit (ICU) of Cipto Mangunkusumo Hospital, Jakarta, Indonesia.METHODS: Specimens were obtained from several body sites of adult patients with infection hospitalised in ICU of Cipto Mangunkusumo Hospital. VITEK®2 was used to identify the microorganisms. Antibiotic susceptibility tests were conducted using VITEK®2 and disc diffusion technique according to Clinical and Laboratory Standards Institute (CLSI) guidelines. Double disc synergy (DDS) test method was employed to detect AmpC activity. Gene families of ampC were identified using multiplex polymerase chain reaction (PCR).RESULTS: Forty five isolates were identified as putative AmpC, extended-Spectrum β-lactamases (ESBL) and AmpC/ESBL-producing Enterobacteriaceae. Klebsiella pneumoniae (n=32) were predominant, followed by Escherichia coli (n=6), Enterobacter cloacae (n=5) and Enterobacter aerogenes (n=2). AmpC activity was detected in 9 isolates, in which 4 isolates were AmpC producing and 5 isolates were AmpC/ESBL. In vitro, AmpC-producing Enterobacteriaceae showed good susceptibility to many antibiotic tested, while those of AmpC/ESBL-producing only to Amikacin. The gene families of ampC were DHA, EBC and CIT identified from 6 isolates.CONCLUSION: DHA, EBC and CIT gene families were identified from AmpC and AmpC/ESBL-producing Enterobacteriaceae in the ICU of Cipto Mangunkusumo Hospital. While the AmpC-producing was still susceptible to almost all antibiotics tested, the AmpC/ESBL-producing showed resistant except for Amikacin.KEYWORDS: Enterobacteriaceae, β-lactamases, AmpC, ESBL
Co-Authors Andi Yasmon Anis Karuniawati Athalia Nofera Kurniawan Delly Chipta Lestari Riki Tenggara Rudyanto Sedono Thomas Robertus