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All Journal HAYATI Journal of Biosciences MITRA: Jurnal Pemberdayaan Masyarakat Jurnal Bakti Masyarakat Indonesia
Yasinta Ratna Esti Wulandari
Department Of Biotechnology, Faculty Of Biotechnology, Atma Jaya Catholic University Of Indonesia, Jakarta
Agriculture, Biological Sciences & Forestry Humanities Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Public Health Social Sciences Other

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Journal : HAYATI Journal of Biosciences

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Detection of Mulatexin in Local Mulberry Plants (Morus spp.) from Bogor, West Java, Indonesia Yasinta Ratna Esti Wulandari; Yogiara Yogiara; Isabel Livena Young
HAYATI Journal of Biosciences Vol. 28 No. 3 (2021): July 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.3.193

Abstract

The mlx56 gene family is found exclusively in the mulberry (Morus spp.) plant, and its role is to defend against insect herbivores, yet it had never been discovered from Indonesia, which has been cultivating mulberry silkworm's (Bombyx mori) feed in order to produce silks. This research focused on studying the expression of mlx56 gene family in local mulberry plants from Bogor, West Java, using different plant organs which were leaf, stem, and root. Each sample was collected from four species of mulberry; Morus alba var. kanva-2, M. alba var. multicaulis, M. bombycis var. lembang, and M. cathayana. Genomic RNA samples were collected and synthesized into cDNA, amplification was done using eight primers of the mlx56 gene family and the products were sequenced. Sequences were then analyzed by BLAST. Results showed diverse mlx56 gene family expression among species of mulberry and plant organs. The mlx56-2, mlx56-4, and mlx56-7, were mostly expressed in all species and organs. The gene mlx56-3, which was not discovered in the previous study, was expressed in M. cathayana leaf and root also in the roots of other species. It was identified as chitinase-like protein LA along with mlx56-5, while other genes were identified as mlx56 based on BLAST.
Co-Authors Agustin Wydia Gunawan Anastasi Tatik Hartanti Dionysius Subali Isabel Livena Young Rory Anthony Hutagalung Tati Barus Yogiara Yogiara