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Journal : Media Penelitian dan Pengembangan Kesehatan

KEMAMPUAN BACILLUS LICHENIFORMIS DALAM MEMPRODUKSI ENZIM PROTEASE YANG BERSIFAT ALKALIN DAN TERMOFILIK Soeka, Yati Sudaryati; Rahayu, Sri Hartin; Setianingrum, Ninu; Naiola, Elidar
Media Penelitian dan Pengembangan Kesehatan Vol 21, No 2 Jun (2011)
Publisher : Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/mpk.v21i2 Jun.109.

Abstract

The aim of the research was to measure the production of protease enzyme in alkaline and thermophilic of bacteria Bacillus licheniformis. The protease enzyme signed by clear zone arround the bacteria colonies on medium which contain 1% skimmed milk. The activities of protease enzyme treated  by the period of incubation, temperature and pH, which measured by spectrophotometer at ? 280 nm. The results showed that the highest production of protease activity at 2 days incubation was 150,52 U/mL. At temperature 50°C and pH 10 they were 123,34  U/mL and 193,14 U/mL.