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Biological Activity of Enzymatically Synthesized Polyphenol Glycoside on Microbial Growth Soeka, Yati Sudaryati; Widiasih, Lutfi Erlita; Sulistyo, Joko; Joko Sulistyo
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (242.37 KB) | DOI: 10.14203/jbi.v3i3.3467

Abstract

ABSTRACTBiological Activity of Enzymatically Synthesized Polyphenol Glycoside on Microbial Growth. We have studied an indigenous bacterial strain produced a glycosyl transfer enzyme (CGTase) yielding polyphenol glycosides from a substrate of starch and polyphenol-aglycone. We observed that the CGTase derived from culture filtrate of some microbial strains (Candida rugosa, Bacillus megaterium, B. coagulans and B. polymixa) could synthesize transfer products in the presence of appropriate polyphenol-aglycones as their acceptors. An inhibitory effects of enzymatically synthesized polyphenol glycosides against bacterial growth was furthermore examined. It was found that polyphenol-glycoside, as one of the transfer products, exhibited high antibacterial activity on the growth of Bacillus subtilis and Escherichia coli, no effect when on Bacillus cereus.Key words : Cyclodextrin glucano transferase (CGTase), enzymatic transglycosylation,polyphenol glycoside, antibacterial activity.
Profil Vitamin, Kalsium, Asam Amino dan Asam Lemak Tepung Jewawut (Setaria italica L.) Fermentasi Soeka, Yati Sudaryati; Sulistiani, Sulistiani
JURNAL BIOLOGI INDONESIA Vol 13, No 1 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1405.736 KB) | DOI: 10.14203/jbi.v13i1.3098

Abstract

ABSTRACTFoxtail millet (Setaria italica L.) is tropical cereal grains of Poaceae. Foxtail millet starch content is quite high, so it has the potential to be used as food raw material; This study has been conducted by making foxtail millet flour fermented with starter bacteria of cellulolytic and amylolytic Bacillus amyloliquifaciens B7 and lactic acid bacteria of Lactobacillus plantarum SU-LS537 which can degrade phytic acid. Parameters measured in the fermentation of foxtail millet was amount of vitamin E, B6 and B12, calcium, essential and non essential amino acids, essential and non essential fatty acids. Fermented foxtail millet decreased vitamin content. A ten fold increase content of calcium concentrations, essential amino acids (histidine, threonine, valine, methionine, isoleucine, leucine, phenylalanine, lysis), non-essential amino acids (aspartic acid, glutamic acid, serine, glycine, arginine, alanine, proline, tyrosine, and cysteine), the fatty acid (lauric , palmitic) and decrease of fatty acid stearic (non essential fatty acids). Bacillus amyloliquifaciens B7 fermentation increased oleic acid but it decreased linoleic acid while Lactobacillus plantarum SU-LS537 fermentation increased linoleic acid, but it decreased oleic acid.Keywords: jewawut (Setaria italica L.), flour, fermentation, Bacillus amyloliquifaciens B7, Lactobacillus plantarum SU-LS537
Optimasi Enzim ?-Amilase dari Bacillus amyloliquefaciens O1 yang Diinduksi Substrat Dedak Padi dan Karboksimetilselulosa Soeka, Yati Sudaryati; Rahmansyah, Maman; ., Sulistiani
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1523.768 KB) | DOI: 10.14203/jbi.v11i2.2200

Abstract

 ABSTRACTBacterial code O1 had been isolated from the leaven of fermented cassava. Based on molecular analysis by partial sequences of 16S rDNA and the phylogenetic character interpretation with Neighbor Joining Method, the strain was identified as Bacillus amyloliquefaciens O1. Bacterial enzymatic activity of ?-amylase was clarified due to the affect of temperature and pH, and as well as its enzymatic stability to convert 2% soluble starch in 100 ml standard media. Aim of the study was to provide benefit in regard on ?-amylase application as crude enzyme extract from the bacteria. In this study, the bacterial strain was being activated to produce ?-amylase by modifying substrates containing cassava starch, rice bran (RB), and carboxymethylcellulose (CMC) in five times volumes (500 mL) of the first scale setting in the standard media.  The result, reducing sugar as a result of enzymatic activity process increased 40 and 55 times in the modified media containing RB and CMC, respectively after 24 hours incubation. In the next 24 hours observation, enzyme activity in bacterial culture based on the RB media was able to degrade amylum in the muslin material containing amylum which was plunged in the media, 1.23 times higher compared to bacterial culture based on the CMC media. Media formula used in the study was able to induce extracellular enzyme activity as well as bacterial culture growth. Keywords: ?-amylase, Bacillus amiloliquefaciens, rice bran, carboxymethylcellulose 
PENERAPAN TEKNOLOGI FERMENTASI PADA BIOPROSES FERMENTASI MINYAK KELAPA (FERMIKEL) [Bioprocessing of Fermented Coconut Oil by Application of Fermentation Technology] Joko Sulistyo; Sulistyo, Joko; Soeka, Yati Sudaryati; Triana, Evi; Napitupulu, Rostiati NR
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (422.461 KB) | DOI: 10.14203/beritabiologi.v4i5.1246

Abstract

Methods of extracting oil from coconut endosperm by fermentatbn were studied. The factors which must be controlled to break the emulsion and liberate oil were investigated. It was found that grinding conditions exerted a profound effect upon the stability of the coconut milk emulsion. The optimum condition for rapid fermentathn of coconut milk was related to the condition during incubation period. The fermentation progressed best under mild conditions (28°C-40°Cj. The fermentation was successful in breaking the emulsion at a relatively broad of range and titrable acidity. Coconut cream and small volume of coconut water and "lontar" (palmyra palmj-sap were incubated separately with some strains of Bacillus species, which were preincubated in a coconut tomato-extract sugar (CTSj medium using a shaker, and grown as a starter under conditions that allowed for coconut oil production at pH 4,0-5,0 and 30 C°- 40 "C for 12-24 h. The organism destabilizes the emulsion, apparently by metabolizing sugars, resulting in the production of protein curd and high-quality oil. The palm sap and coconut water to the cream ratio of fermentation medium influenced the performance of oil produced and the bacteria grew well and produced oil in non sterile systems. The oil recovered was about 25 to 20% while average amount of oil in the coconut is approximately 25-35%, which means that only 83,33 to 66,67% oil was recovered. The oil contained little free fatty acid and very low concentration of cholesterol (0,0095 mg/ml), while the traditional coconut oil and commercially palm oil were 0,0111 mg/ml and 0,0132 mg/ml, respectively.
OPTIMASI DAN KARAKTERISASI a-AMILASE DARI ISOLAT AKTINOMISETES YANG BERASAL DARI KALIMANTAN TIMUR Soeka, Yati Sudaryati
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (368.489 KB) | DOI: 10.14203/beritabiologi.v10i3.751

Abstract

Forty-one actinomycetes isolates from East Kalimantan held in Microbiology Division Collection-UPI, and their ability to produce a-amylase has been assessed. Those 41 number of actinomycetes isolates performed amylolytic activity as shown by clear zone areal after being poured with iodium solution. The bacteria produced high a-amylase when was grown in media containing starch soluble 2% the a-amylase activity in media containing 8.24 U/ml. The isolate (number 7) was the most active compared to another (number 100) and it was identified as Nocardia; the activity of this enzyme obtained was 12.93 U/ml (one unit activity is defined as mol of glucose produced per ml per minute). The maximum temperature for enzyme reaction was 40°C, optimum pH was pH 7.5 the a-amylase activity were 15.76 U/ml and 31.11 U/ml, respectively. From kinetic characterization study, it was found that enzyme showed Km and Vmax value of 7.62 % (b/v) and 71.10- umol/ml/minute respectively at condition of temperature 40°C, pH 7.5 and incubation time 10 minute.
EKSTRAKSI MINYAK NABATI SECARA FERMENTASI Soeka, Yati Sudaryati
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (417.466 KB) | DOI: 10.14203/beritabiologi.v9i3.789

Abstract

Method of extracting vegetable oil from peanut and soybean cream through enzymatic fermentation was studied. Creams were incubated with respective strains of Bacillus sublilis, Candida rugosa and Pseudomonas aerogenes separately under mild condition that allowed for production of oil at pH 4.5-6.5 and room temperature overnight. A considerably amount of oil could be extracted from media containing coconut and peanut cream, however, none from soybean cream.The oil recovery was about 10 to 30% from approximately 46 to 52% total fat contained in the respective substrates.Those oil were extracted by application of culture filtrate of B. subtilis and C. rugosa exhibited higher linoleic and Iinolenic acid (0.50 to 0.61 % and 0.31 to 0.32% respectively), compared to those were of P. aerogenes lipase (0.20% and 0.13%, respectively).Further investigation was aimed to study the capacity of microbial strains on enzymatic reaction of fatty acid to fatty acid ester by incubating extracted oil in the present of organic solvents.
AKTIVITAS ANTIMIKROBA FLAVONOID - GLIKOSIDA HASIL SINTESIS SECARA TRANSGLIKOSILASI ENZIMATIK Soeka, Yati Sudaryati; Naiola, Elidar; Sulistyo, Joko; Joko Sulistyo
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (617.43 KB) | DOI: 10.14203/beritabiologi.v8i6.825

Abstract

Flavonoid-glycoside was synthesized enzymatically using CGT-ase (EC.2.4.1.19) of indigenous Bacillus licheniformis in a phosphate buffer pH 6.0 at 45°C for 24 h, through transglycosylation reaction in the present of flavonoid those were extracted from rhizomes such as ginger, flngerroot, turmeric, white turmeric and white curcuma as natural acceptors, and commercial rice,cassava, corn and wheat flour as substrates.The result showed that CGT-ase of B. licheniformis transferred a glycosyl moiety in a bilayer enzymatic reaction system of n-hexanol and phosphate buffer yielding glycosides as transfer products in the present of wheat flour as substrate and white curcuma extract as its acceptor.An inhibitory effects of the synthesized flavonoid glycosides against microbial growth was furthermore examined. It was found that flavonoid-glycoside, as the transfer product, exhibited high antimicrobial activity at MIC 200 ppm on the growth of Bacillus subtilis, Escherichia coli and Saccharomyces cerevisiae, however no effect when it was assayed on Candida tropicalis, while arbutin and flavonoid-aglycon showed very low inhibitory activity on the growth of two out of four tested microbial strains.
KEMAMPUAN BACILLUS LICHENIFORMIS DALAM MEMPRODUKSI ENZIM PROTEASE YANG BERSIFAT ALKALIN DAN TERMOFILIK Soeka, Yati Sudaryati; Rahayu, Sri Hartin; Setianingrum, Ninu; Naiola, Elidar
Media Penelitian dan Pengembangan Kesehatan Vol 21, No 2 Jun (2011)
Publisher : Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/mpk.v21i2 Jun.109.

Abstract

The aim of the research was to measure the production of protease enzyme in alkaline and thermophilic of bacteria Bacillus licheniformis. The protease enzyme signed by clear zone arround the bacteria colonies on medium which contain 1% skimmed milk. The activities of protease enzyme treated  by the period of incubation, temperature and pH, which measured by spectrophotometer at ? 280 nm. The results showed that the highest production of protease activity at 2 days incubation was 150,52 U/mL. At temperature 50°C and pH 10 they were 123,34  U/mL and 193,14 U/mL.
Seleksi, Karakterisasi, dan Identifikasi Bakteri Penghasil Kitinase yang Diisolasi dari Gunung Bromo Jawa Timur Soeka, Yati Sudaryati; Sulistiani, Sulistiani
Jurnal Natur Indonesia Vol 13, No 2 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (72.571 KB) | DOI: 10.31258/jnat.13.2.155-161

Abstract

Selection, characterization and identification of bacteria that can produce chitinase enzyme were isolated fromBromo Mountain, East Java. The 48 isolates were tested for capability to degrade chitine qualitatively, semiquantitatively and quantitatively. The result showed that 2 isolates, B2-4 and NA S4-1 could degrade chitin, withactivities of 4.8. 10-3 and 3.1 . 10-3 U/ml, after 1 and 2 days incubation respectively. By using molecular characterizationmethods, partial sequences of 16S rDNA and the primers 9F & 1510R were identified as Stenotrophomonas sp.
Aktivitas Antimutagen Isoflavon Glikosida Hasil Transglikosilasi Enzimatik CGT-ase Bacillus macerans Sulistyo, Joko; Soeka, Yati Sudaryati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 1 (2009): February 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (278.276 KB) | DOI: 10.24002/biota.v14i1.2629

Abstract

It has been known that isoflavone have biological activities such as antioxidant, antibacteria, antimutagenesis, and anticancer. Isoflavone aglycone uses such as genistein, daidzein and glycitein are limited since they are unstable and uneasily to dissolve in water. Through enzymatic transglycosylation reaction, its stability and solubility could be improved. In this study, genistin (isoflavone glycoside) was synthesized from genistein (isoflavone aglycone) by application of transfer reaction using enzyme cyclodextrin glucanotransferase (CGT-ase) of Bacillus macerans. Identification of the product was determined by TLC with methanol: chloroform (1:3, v:v) as eluent. Rf value 0.75 of the synthesized product was close to the Rf value standard of authentic genistin glycoside. The synthesized genistin was furthermore assayed to determine its antimutagenesis activity according to the Ames methode on E. minimal glucose media had been precultured with a mutant strain of Salmonella thypimurium TA98. The tested bacterial strain was induced with aflatoksin B1 as mutagen which had been activated with a lever homogenate. The result showed that the solubility and some biological properties of the synthesized genistin were improved higher than that of genistein, while it was found to be lower than that of the commercial genistin.