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All Journal Indonesian Journal of Natural Pigments
Adhiwibawa, Marcelinus Alfasisurya Setya
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Chemistry Other

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Statistical Analysis for Evaluating Natural Yellow Coloring Agents from Peel of Local Fruits in Malang: Mangosteen, Honey Pineapple and Red Dragon Fruits Kurniawan, Yehezkiel Steven; Adhiwibawa, Marcelinus Alfasisurya Setya; Setiyono, Edi; Fahmi, Muhammad Riza Ghulam; Lintang, Hendrik Oktendy
Indonesian Journal of Natural Pigments Vol 1 No 2 (2019): Agustus 2019
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2019.01.2.49

Abstract

In the present work, a comprehensive statistical analysis was performed to evaluate the potential application of peel of local fruits from Malang, i.e. mangosteen, honey pineapple and red dragon fruits for natural yellow coloring agents. The yellow pigments from those fruit peels were extracted through a simple maceration method using distilled water, acetone and ethanol as the solvents. The CIE color space of the extracts was measured to obtain L*, a* and b* values. The obtained data were further analyzed using Principal Component Analysis (PCA), Multivariate Analysis of Variance (MANOVA) and Duncan Test to determine the most potent natural yellow coloring agent. All the extracts were appeared as mild to strong yellow liquid except for acetone extract for the peel of red dragon fruit extracts. From the CIE color space and PCA analysis, either ethanolic or acetone extracts of mangosteen appears as a strong yellow liquid and they are statistically not different. Interestingly, the MANOVA and Duncan test results are able to distinguish that the ethanolic extract of mangosteens’ peel as the best candidate for natural yellow coloring agents because of its lowest L* and also highest b* variable values.
Characterization of Tambjamines Pigment from Marine Bacterium Pseudoalteromonas sp. PM2 Indigenous from Alor Island, Indonesia Setiyono, Edi; Adhiwibawa, Marcelinus Alfasisurya Setya; Prabowo, Matheus Randy; Brotosudarmo, Tatas H.P.
Indonesian Journal of Natural Pigments Vol 3 No 1 (2021): February 2021
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2021.03.1.16-23

Abstract

Pigments from marine bacteria have attracted the attention for scientists because of their extensive applications and currently exploration of new pigment sources from marine bacteria is still ongoing. Recently, we have successfully isolated six new yellow-pigmented marine bacteria, strain PS2, PM2, SB11, SB13, SB21, and SB23, isolated from seawater from different sampling sites on Alor Island, Indonesia. The UV−Vis and FTIR spectra of the crude pigment extracts of the six strains showed the characteristics of tambjamines, a group of yellow pigments commonly found in nudibranchs and bryozoans. Moreover, separation and characterization of crude tambjamines extract resulted in five different types of tambjamine with maximum absorbance at the wavelength of 374−392 nm. Based on the analysis of 16S rRNA gene sequences, strain PM2 was closely related to several species in genus Pseudoalteromonas with a similarity of more than 99%. Strain PM2 was designed as Pseudoalteromonas sp. PM2 with accession number LC505058. So far, only two marine bacteria have been known to produce tambjamine and they are from genus Pseudoalteromonas. Our new finding indicated that in the group of marine bacteria, tambjamine might be only synthesized by members from genus Pseudoalteromonas.
Spectroscopy Study of Honey Pineapple Peels Extracted in Different Solvents Kurniawan, Yehezkiel Steven; Setiyono, Edi; Adhiwibawa, Marcelinus Alfasisurya Setya; Priyangga, Krisfian Tata Aneka; Yuliati, Leny
Indonesian Journal of Natural Pigments Vol 3 No 1 (2021): February 2021
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2021.03.1.32-35

Abstract

In the present work, we investigated the extract of honey pineapple peels in distilled water, ethanol, and acetone solvents. The spectroscopy study of each extract was performed using a Fourier transform infrared (FTIR) spectrometer, an ultraviolet-visible (UV-Vis) spectrophotometer, and a spectrofluorometer. The FTIR spectrum of the distilled water extract indicated that the distilled water extract may contain alcohol or carboxylic acid compounds. Meanwhile, the ethanolic extract may contain alcohol or carboxylic acid, or ether compounds. On the other hand, the acetone extract may contain alcohol or ether or aromatic or aliphatic compounds. The UV-Vis spectrum of the honey pineapple peels extracted in the distilled water, ethanol, and acetone showed a broad absorption signal at UV region (< 300 nm), four absorption signals at UV region (232-368 nm), and four absorption signals at UV region (231-368 nm) with a weak absorption signal at the visible region at 559 nm, respectively. The distilled water and acetone extracts gave fluorescence signals, however, the ethanolic extract showed no fluorescence intensity. From the FTIR, UV-Vis, and fluorescence spectra characterization, the extracted natural pigments from the honey pineapple peels in distilled water, ethanol, and acetone solvents were identified. The distilled water extract may contain polar flavonoid or steroid compounds while the ethanolic extract may contain polar carotenoid pigments. On the other hand, the acetone extract may contain carotenoid and chlorophyll pigments as shown by an emission signal at 670 nm.
Co-Authors Edi Setiyono Fahmi, Muhammad Riza Ghulam Kurniawan, Yehezkiel Steven Leny Yuliati Lintang, Hendrik Oktendy Prabowo, Matheus Randy Priyangga, Krisfian Tata Aneka Tatas H.P. Brotosudarmo