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Hartati, H.
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Education

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Perbedaan Ekstrak Etanol dan Etil Asetat Daun Lada (Piper nigrum L) terhadap Aktivitas Antimikroba Hartati, H.; Pagarra, Halifah
Sainsmat : Jurnal Ilmiah Ilmu Pengetahuan Alam Vol 7, No 1 (2018): Maret
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (701.292 KB) | DOI: 10.35580/sainsmat7164702018

Abstract

Penelitian ini bertujuan untuk mengetahui perbedaan ekstrak etanol dan ekstrak etil asetat daun lada (Piper nigrum L) terhadap aktivitas antimikroba Staphylococcua aureus, Escherichia coli dan Candida albicans. Metode maserasi telah digunakan untuk mengekstrak daun lada dengan menggunakan dua pelarut yaitu etanol 70% dan etil asetat. Hasil ekstrak selanjutnya dilakukan uji aktivitas antimikroba. Hasil ekstrak etanol daun lada diperoleh rendemen yang lebih tinggi yaitu 24,64% dibandingkan dengan ekstrak etil asetat 9,18%. Ekstrak etanol daun lada memiliki aktivitas antimikroba lebih tinggi dibandingkan ekstrak etil asetat daun lada terhadap bakteri Staphylococcua aureus,dan Escherichia coli tetapi tidak menghambat jamur Candida albicans  pada konsentrasi 10%.
Pengaruh Jenis Pelarut terhadap Kandungan Senyawa Metabolit Sekunder Klika Kayu Jawa (Lannea coromendelica) Hartati, H.; B, Syamsuddin; Karim, Hilda
Sainsmat : Jurnal Ilmiah Ilmu Pengetahuan Alam Vol 8, No 2 (2019): September
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (875.533 KB) | DOI: 10.35580/sainsmat82107162019

Abstract

Kayu Jawa (Lannea coromendelica) merupakan tumbuhan yang tersebar di berbagai daerah dan memiliki potensi untuk dieksplorasi dalam pemanfaatannya. Penelitian ini bertujuan untuk mengeksplorasi Kayu Jawa (Lannea coromendelica) dengan menentukan jenis pelarut dan cara ekstraksi terbaik dalam mengekstraksi kandungan senyawa metabolit sekunder klika Kayu Jawa (Lannea coromendelica). Ekstraksi di lakukan dengan metode maserasi menggunakan pelarut etil asetat dan etanol 70% serta menggunakan sampel kering dan basah, sehingga di peroleh 4 macam ekstrak yaitu Ekstrak etanol 70% klika Kayu Jawa basah (KJBE70%), ekstrak etanol 70% klika Kayu Jawa kering (KJKE70%), ekstrak etil asetat klika Kayu Jawa kering (KJKEA), dan ekstrak klika Kayu Jawa basah (KJBEA). Senyawa metabolit sekunder yang terdapat pada Kayu Jawa (Lannea coromendelica) adalah flavonoid, saponin, tanin, dan triterpenoid. Ekstrak klika Kayu Jawa basah (KJBEA) memiliki kandungan senyawa metabolit sekunder tertinggi sebanyak 3 senyawa yaitu flavonoid, saponin, dan tanin.
Genetic polymorphism of Pit-1|HinfI gene in Grati-Ongole Grade cattle at Indonesian Beef Cattle Research Station Hartati, H.; Anwar, S.; Soewandi, B.D.P.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 4 (2018): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.4.315-322

Abstract

The pituitary transcription factor (Pit-1) gene is one of the considered genes that responsible to growth in cattle. A specific Pit-1|HinfI gene polymorphism located within exon 6(g.1256G>A) has been shown to be associated with growth and milk traits in cattle. This study aimed to identify the Pit-1|Hinf1 gene polymorphism in Grati-Ongole Grade cattle (Grati-OG cattle). A total of 107 genomic DNA of Grati-OG cattle were used in this study. The detection of polymorphism was performed by PCR-RFLP method. Only AB and BB genotypes were succesfully identified with the frequency of 0.009 and 0.991, respectively. Whereas, the frequency of A and B alleles were 0.005 and 0.995, respectively. The observed genotype frequencies in this population do not deviated from HWE. The value of Ho, He and PIC were 0.009, 0.009 and 0.009, respectively.In conclusion, the Pit-1|HinfI gene polymorphism in Grati-OG cattle is monomorphic and hence it should not be used in further association studies.
Association of GHR|AluI gene polymorphism with body weight parameters in Grati-PO cattle Hartati, H.; Hapsari, A.A.R.; Soewandi, B. D. P.; Anwar, S.; Rahmadani, S. P.; Aryogi, A.; Pamungkas, D.
Journal of the Indonesian Tropical Animal Agriculture Vol 46, No 4 (2021): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.46.4.272-281

Abstract

The growth hormone receptor (GHR) gene is member of the class I cytokine receptor family that important in growth regulation including in cattle. This study aimed to analyze the association between GHR|AluI gene with body weight, parameters in Grati-PO cattle population. A total of 304 blood samples were collected from the experimental station at the Beef Cattle Research Station, which were also equipped with 304 phenotypic data. DNA was extracted using a zymo extraction kit. Detection of of the polymorphism of GHR gene using PCR-RFLP method with AluI restriction enzymes. polymorphism indicators include genotype frequency, allele frequency, Hardy-Weinberg Equilibrium, observed heterozygosity (Ho), expected heterozygosity (He), polymorphism information content (PIC) and number of effective alleles (Ne). Association analysis was carried out using a linear mixed model. The performance of birth weight, weaning weight, yearling weight and 18 months weight were 25.4 + 0.2 kg; 113.8 + 2.3 kg; 159.9 + 2.7 kg and 213.7 + 3.6 kg, respectively. The result of association analysis was significant (P<0.05) on weaning weight, yearling weight and 18 months weight. It could be concluded that the GHR gene has potential to be a candidate genetic marker for growth traits in Grati-PO cattle.
Co-Authors Aryogi, A. B, Syamsuddin Halifah Pagarra Hapsari, A.A.R. Hilda Karim Pamungkas, D. Rahmadani, S. P. S. Anwar Soewandi, B. D. P. Soewandi, B.D.P.