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All Journal Jurnal Gizi Klinik Indonesia Indonesian journal of health sciences research and development
Banudi, La
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Health Professions Public Health

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The effect of Sea urchin (Diadema setosum) gonad extract on IgM and IgG antibodies production in BALB/c mice infected by Salmonella typhi Salma, Wa Ode; Yusuf, Ilyas; Karo, Marni; Banudi, La
Jurnal Gizi Klinik Indonesia Vol 14, No 3 (2018): Januari
Publisher : Minat S2 Gizi dan Kesehatan, Prodi S2 IKM, FK-KMK UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (136.15 KB) | DOI: 10.22146/ijcn.26834

Abstract

Background: Salmonella typhi infection decreases of the immune system and influences the adaptive antibodies among malnourished children. The gonad of Diadema setosum (D. setosum) is one of food sources from marine biota that contains high-quality nutrients and potentially can be used as a dietary supplement for typhoid fever condition.Objective: This study aimed to determine the effect of gonad D. setosum extract on the production of antibody IgM and IgG in an animal model.Method: This experimental study was used BALB/c mice before and after infected Salmonella typhi through intraperitoneally at 0.2 mL x the unit 103 CFU/mL. The level of IgM and IgG production was measured by Enzyme Linked Immune Sorbent Assay (ELISA). Experimental animals were divided into 2 groups. The control group was only fed with standard diets, while at the intervention group received the extract of D. setosum gonad in two doses (100 and 200 mg/kg body weight).Results: Production of IgM antibodies in the control group significantly increased twofold (p=0.001) whereas the intervention group received the extracts of D. setosum gonad (200 mg/kg body weight) could suppress the increase in IgM antibody production and indicate the highest increase of IgG antibody significantly (p<0.05) at day 7.Conclusion: The gonad of Diadema setosum extracts (200 mg/kg body weight) could suppress the increase in IgM antibody productions and indicate the highest increase of IgG antibody titers in mice infected with Salmonella typhi. The role of anti-microbial substances of the gonad of Diadema setosum, is potential to be utilized as dietary supplement to increase body immune system among patients infected by Salmonella typhi.
PROVISION OF SUPPLEMENTARY FOOD FOR SASIGO AND BISCUIT IN INCREASING THE WEIGHT OF CHILDREN : A COMPARATIVE STUDY IN NAMBO PRIMARY HEALTH CARE IN KENDARI CITY: Supplementary Food for Children Rasyid, Mariyatni; Timbul Supodo; Banudi, La
INDONESIAN JOURNAL OF HEALTH SCIENCES RESEARCH AND DEVELOPMENT (IJHSRD) Vol. 2 No. 3 (2020): INDONESIAN JOURNAL OF HEALTH SCIENCES RESEARCH AND DEVELOPMENT
Publisher : Universitas Mandala Waluya Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36566/ijhsrd/Vol2.Iss3/53

Abstract

Background:The prevalence of community nutritional status in the Kendari City area in 2016 was 0.23%. In 2017, the prevalence of nutritional status decreased in 0.13% and then increased in 2018 by 4.67%. in 2019, the prevalence of nutritional status decreased by 2.25% . Thus,  children under five have nutritional status did not reached the target This study aimed to understand the effect of giving the sasigo and biscuit on increase the children’s weight in Nambo Primary Health Care in Kendari City. Methods:The type of research in this research is pre-experimental research with a Pre Test - Post Test design. This research was conducted in the working area of ??Nambo Peimary Health Care, Kendari City. Population were 227 and a sample was  67 malnourished toddlers in 2019. Result:Based on the independent sample test output in the Equal Variances Assumed section on Local PMT (BageaSasigo), it is known that the sig (2-tailed) value is 0.001 &lt;0.05, so Ha is accepted so that there is a significant difference between the provision of local PMT (BageaSasigo). Whereas in the Government PMT (biscuits) it is known that the sig (2-tailed) value is 0.000 &lt;0.05, so Ha is accepted so that there is a significant difference between the provision of government PMT (biscuits) to toddlers in the Work Area of ??the PuskesmasNambo, Kendari City. Conclusion:Provision of Local PMT (Bagea Sasigo) and Government PMT (Biscuits) for toddlersBefore giving the intervention it was not effective while after giving the intervention it was effective and significant.
Co-Authors Karo, Marni Rasyid, Mariyatni Timbul Supodo Wa Ode Salma Yusuf, Ilyas