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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Bioeksperimen: Jurnal Penelitian Biologi
Gunawan, Yohanes Edy
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Agriculture, Biological Sciences & Forestry Education Environmental Science

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The Role of Coenzymes on Mercury (Hg2+) Bioremediation by Isolates Pseudomonas aeruginosa KHY2 and Klebsiella pneumonia KHY3 Neneng, Liswara; Gunawan, Yohanes Edy
Journal of Tropical Life Science Vol 8, No 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (581.306 KB) | DOI: 10.11594/jtls.08.01.04

Abstract

Mercury pollution is dangerous to health. Previous research was found two potential Gram-negative bacteria for mercury bioremediation, from gold mining in Central Kalimantan, Indonesia.  These isolates were identified as Pseudomonas aeruginosa KHY2 and Klebsiella pneumonia KHY3. Mechanisms of mercury bioremediation had not known yet by these isolates.  This study purposed to test the role of coenzymes on mercury bioremediation by these isolate and to determine the coenzymes best level of mercury bioremediation. Experimental design was Completely Randomized Design in a laboratory.  Treatment factors were coenzymes obtained from vitamins B1, B6, B12, with 6 levels of treatments, included 1 control.  All treatments were done in Luria Broth media that contain 12 ppm of mercury. Mercury was measured by AAS Shimadzu AA-6200. The results showed that coenzymes effect was very significant to improve mercury bioremediation by P. aeruginosa KHY2 and K. pneumonia KHY3.  Supplementation of vitamin B12 in culture media, more enhance of mercury bioremediation compared with vitamin B1 and B6. These result above, indicated the mechanism of mercury bioremediation in both isolates, were the enzymatic process.
Linking Zoopharmacognocy with Ethnomedication, An Evidence Base from Sebangau National Park, Central Kalimantan Indonesia Panda, Adventus; Gunawan, Yohanes Edy
Journal of Tropical Life Science Vol 8, No 3 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.03.15

Abstract

The ability of animal to perform self-medication has been studied decades, as well as their relationship with medication practices by communities. Long-term observation of Orangutans' behaviour (Pongo pygmaeus wurmbii), by communities surround Sebangau National Park, has suggested as their modes to today ethno-medication practice. The study was aimed to study the relationship of Orangutans self-medicate behavior with ethno-medication practice, in Sebangau area. The research was conducted in Punggualas, SNP, from 29 April to 03 October 2017. A number of 13 traditional healers (TH) from Karuing (n = 4), Baun Bango (n = 4), and Jahanjang (n = 5) have been interviewed. All plants are listed and photograph. Plants parts, and their mechanism of utilization were also kept for record. Meanwhile, the behavior followed the Orangutan protocol, with focus on their feeding behavior. All data were analyzed descriptively, while the relationship was analyzed using chi-square and F test. We have recorded a total 131 plants at various life forms, in Baun Bango (n = 59), Jahanjang (n = 41), and Karuing (n = 21). Plants that were found similar among three villages, removed, were only 95 left. We observed one female, showing the ability to perform self-mediaction. It is characterized by selectively choosing young leaves of Mezzetia sp., pulp of the Dyera lowii and Ilex cymosa, and lastly chew the entire leaves of Belang Handipek. It suggests a form of prevention against fatigue conditions, and the combination of these three plants species assume to be relating to fitness. This study shows that there is a relationship between the plant part used by the traditional healer and the orang- utan (x2 = 43,887; n = 115, df = 11, p-value = 0.0000), the relationship between the use of plant parts utilized by the traditional healer and orangutans (x2 = 15, 647; n = 50, df = 8, p-value = 0.0000). Furthermore, there is a relationship between the practice of traditional healer treatment using plant parts and Orang-utan (F1, 113 = 230.158; p-value = 0,000). The study urges to isolate secondary metabolites for further investigation, especially in terms of phyto-pharmacy.
Identifikasi Jenis Kupu-Kupu (Lepidoptera) Di Universitas Palangka Raya Gonggoli, Ade Damara; Sari, Sartika; Oktofiani, Helen; Santika, Novira; Herlina, Rini; Agatha, Tiara; Gunawan, Yohanes Edy
Bioeksperimen: Jurnal Penelitian Biologi Vol 7, No 1: Maret 2021
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v7i1.10361

Abstract

Tujuan penelitian adalah untuk mengidentifikasi jenis kupu-kupu yang berada di Universitas Palangkaraya pada lahan yang setelah terbakar dan lahan yang belum terbakar. Metode penelitian menggunakan metode observasi lapangan dengan teknik net sweeping dan jelajah. Dari hasil identifikasi jenis kupu-kupu yang dilakukan di sekitar kawasan UPR menunjukkan bahwa jumlah total jenis kupu-kupu yang ditemukan sebanyak 10 spesies yang tergolong ke dalam empat famili masing-masing yaitu famili Nymphalidae (3 jenis), famili Pleridae (5 jenis), famili Papilionidae (1 jenis), dan Lycaenidae (1 jenis), dengan total jumlah individu sebanyak 30 ekor, yang di dominasi oleh spesies Acraea terpsicore.
Co-Authors Adventus Panda Agatha, Tiara Gonggoli, Ade Damara Herlina, Rini Liswara Neneng Oktofiani, Helen Santika, Novira Sartika Sari