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All Journal JURNAL SAINS DAN TEKNOLOGI INDONESIA Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Marhendra, Agung P.W.
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Education

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MEKANISME PELEPASAN HORMON GONADOTROPIN (GtH-II) IKAN LELE (Clarias sp) SETELAH DI INDUKSI LASERPUNKTUR PADA TITIK REPRODUKSI P.S.W, kusuma; Marhendra, Agung P.W.; Aulanni’am, Aulanni’am
Jurnal Sains dan Teknologi Indonesia Vol. 14 No. 3 (2012)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (99.672 KB) | DOI: 10.29122/jsti.v14i3.928

Abstract

Induction laserpuncture effectively stimulates gonad maturation and spawning, but the associated gonadotropin releasing hormone (LH / GtH-II) mechanism is still unknown. So the purpose of this study demonstrate the mechanism of the release of GTH-II on catfish (Clarias sp.) after induction laserpuncture at the reproduction acupoint. The test fish consisted of 54 males and 54 females aged 8-9 months from the F1 hybrid Sangkuriang type female and Paiton type male parents. Ourstudy employed an experimental method with completely randomized design. The treatment comprised 6 levels with 9 repetitions. We were to observe gonadotropin hormone (GtH-II) concentrations pre-spawn, spawn, post-spawning in the laserpuncture exposed. As a comparison investigations were also conducted to untreated (control). Blood sampling performed at 6 h after induction laserpuncture. Hormone levels using the Elisa test kits Fish Luteinizing Hormone (LH) BIOTECH CO. CUSABIO., LTD. by Catalog No.. CSB-E15791Fh. The test results significant effect (P<0.05) increased levels of GtH-II profiles between laserpuncture inductionwith control, which profiles GTH-II levels pre-spawn conditions higher 2.2468, as well as the higher spawn condition 1,5409 in comparison with controls. However, the condition post-spawning in induction laserpuncture lower value 1.1131 compared with controls. This suggests that induction laserpuncture at the reproduction acupoint can stimulate of GABA release from GABAergic neurons pre-spawn and spawn condition. GABA stimulates the hypothalamus to release GnRH neurons. GnRH neurons stimulates the pituitary to GtH-II release. GtH-II will affect the acceleration of gonad maturation and spawning.
Sistematik Filogenetik Isolat-isolat Kapang Indigenous Indonesia sebagai Entomopatogen Kutu Sisik (Lepidoshapes beckii Newman) Hama Tanaman Jeruk Suharjono, Suharjono; Marhendra, Agung P.W.; Triwiratno, Anang; Wuryantini, Susi; R, Lina Oktavia
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 2 (2010): June 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (461.827 KB) | DOI: 10.24002/biota.v15i2.2708

Abstract

Citrus fruits constitute a basic agricultural product in many countries. They are a source ofnational income, empowering labor market in rural zones and those surrounding urban areas,and in addition to being an important part of the diet. Insects are common problems to plantingof citrus. Purple Scale, Lepidosaphes beckii (Newman) is one of the destructive insect pests ofcitrus worldwide. The control of purple scale insects in citrus utilizes native natural enemiesincluding pathogens. Most known species of mould are pathogens of purple scale insect of citrus.The objective of this research was to study phylogenetic systematics of Indonesian indigenousmould isolates as pathogen on purple scale insect (Lepidoshapes beckii Newman) pest of citrusplants. Seven mould isolates derived from Purworejo, Brastagi, and Poncokusumo were showedpathogenic on purple scale insect. The fungal isolates were cultured on PDA. The DNA of fungalmaterial was isolated with Kit Prepman Ultra (Applied Biosystems). Nuclear ribosomal LargeSubunit 28S rDNA (LSU) was amplified using primers ITS1 and ITS4. Products of PCR werepurified and sequenced. Based on the 28S rDNA sequence, the result was showed that isolate ofPW ASG D4 was related to Gibberella moniliformis dx-12, PC ORG D4 was related to Gibberella sp.1893, PW ASG D2 was related to Gibberella moniliformis BZ070101, PW LB D2 was related toGibberella moniliformis 52, BRS D22 was related to Fusarium oxysporum DC-1-67, PWRJ I D4 wasrelated to Fusarium culmorum SQ070108, and BRS D23 was related to Aschersonia sp. Ag-11.
Co-Authors Aulanni'am, Aulanni'am Jamhari Jamhari P.S.W, kusuma R, Lina Oktavia Susi Wuryantini, Susi Triwiratno, Anang