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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Mendez, Noe Polo
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Agriculture, Biological Sciences & Forestry Environmental Science

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In vitro Studies on Pollen Viability, Pollen Germination and Pollen Tube Growth of Hornstedtia conoidea Ridl. – a Philippine Endemic Ginger Species Mendez, Noe Polo; Acma, Florfe Macabudbud
Journal of Tropical Life Science Vol 8, No 3 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (436.123 KB) | DOI: 10.11594/jtls.08.03.13

Abstract

Zingiberaceous plants are predominantly propagated through underground rhizomes and seeds. In this study, the viability of pollen, rate of pollen germination and length of pollen tube of the Philippine endemic Hornstedtia conoidea were examined. Four petri plates containing pollen samples were prepared, of which two petri plates were used immediately after the collection, while the other two petri plates were stored for one week. The determination of pollen viability was made employing stain tests using IKI (iodine + potassium iodide) solution and safranin and measured immediately after the collection and after one week. Likewise, pollen germination test was carried out in vitro on agar medium and measured after 24 hours and one week of incubation. Data revealed that pollen viability was higher in the samples which were sown immediately with safranin with percentage pollen viability of 92.43%, while IKI test was lower with 89.36%. On the other hand, the pollen stored for one week measured 47.29% for safranin test and 33.14% for IKI test. The percentage germination of pollen after 24 hours was 72.65%, while 11.13% after one week. The pollen samples which were subjected for tube growth were examined for two weeks and gave positive results. Overall, it can be concluded that H. conoidea pollen can successfully be collected and stored for certain time. Knowledge regarding the pollen viability, pollen germination and pollen tube growth of this species will give practical benefit for plant breeding and conservation purposes, since H. conoidea is found in the wild, and as of now, the species has been recorded only in the Philippines.
Species Richness and Endemism of Zingiberaceae in Cinchona Forest Reserve, Lantapan, Bukidnon, Philippines Jayme, John Austin Lennox Faro; Mendez, Noe Polo; Mendez, Rainear Auxtero; Somera, Daniel F; Mohagan, Alma B
Journal of Tropical Life Science Vol 10, No 2 (2020)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (622.762 KB) | DOI: 10.11594/jtls.10.02.10

Abstract

This study was carried out to provide information on species richness and endemism of Zingiberaceae in Cinchona Forest Reserve, Kaatuan, Lantapan, Bukidnon, Philippines. Transect walks, opportunistic sampling and collection within the sampling quadrats were conducted along established forest trails to collect ginger species. A total of 11 species of Zingiberaceae were documented belonging to two subfamilies (Alpinioideae and Zingiberoideae) and three tribes (Alpinieae, Hedychieae, and Zingibereae). The species recorded include Adelmeria alpina Elmer, Alpinia haenkei C.Presl, A. rufa C.Presl, Etlingera fimbriobracteata (K.Schum.) R.M.Sm., E. pubimarginata (Elmer) A.D.Poulsen, Hedychium philippinense K.Schum., Hornstedtia conoidea Ridl., H. lophophora Ridl., Meistera muricarpa (Elmer) Škorničk. & M.F.Newman, Zingiber banahaoense Mood & Theilade, and Zingiber sp. Of these, H. philippinense is the only threatened species recorded. All species are endemic to the Philippines except for E. fimbriobracteata which is native to Borneo and Zingiber sp. which is unidentified to the species level. These species represent 41% of the total genera and 9% of the total species of Zingiberaceae in the Philippines. The high endemicity (82%) in the total collected species in this study and the presence of a threatened species in this area calls for protection and conservation by the stakeholders.
Co-Authors Acma, Florfe Macabudbud Jayme, John Austin Lennox Faro Mendez, Rainear Auxtero Mohagan, Alma B Somera, Daniel F