Yulinery, Titin
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ANALISIS VIABILITAS PROBIOTIK Lactobacillus TERENKAPSULASI DALAM PENYALUT DEKSTRIN DAN JUS MARKISA (Passiflora edulis) Yulinery, Titin; Nurhidayat, N.
Jurnal Teknologi Lingkungan Vol. 13 No. 1 (2012)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.835 KB) | DOI: 10.29122/jtl.v13i1.1411

Abstract

Probiotik cair tidak efisien dalam stabilitas dan kemasan. Bubuk probiotik dapat disimpan lebih lama dan stabilitas dalam kualitas. Bubuk bisa dibuat dengan metode enkapsulasi sedangkan konsentrasi lapisan sangat penting. Rasa dari bubuk seperti jus markisa yang diperlukan untuk mendapatkan rasa bagus di minum. Penelitian ini menggunakan L. plantarum dan Lactobacillus Mar 8 A17 dengan menambahkan gairah jus buah dan dekstrin dengan konsentrasi beberapa seperti 2%, 5%, 10% dan 15%. Kemudian dikemas dengan menggunakan spray dryer pada 125oC. Bubuk dari enkapsulasi disimpan selama 2 minggu pada suhu 4oC, tahapan uji coba telah dilakukan untuk bedak sebelum dan setelah enkapsulasi setelah disimpan selama 2 minggu pada suhu 4oC. Setelah enkapsulasi bahwa berat tertinggi pada pengobatan dengan menggunakan jus buah gairah Lactobacillus A17 dengan dekstrin 15% adalah 5649,6 mg dan diikuti oleh L. plantarum Mar8 adalah 5400 mg. Kelangsungan hidup setelah enkapsulasi untuk setiap konsentrasi dekstrin yang signifikan, kecuali pada konsentrasi 10% tidak signifikan, tapi di gudang untuk penyimpanan dua minggu setelah enkapsulasi kelangsungan hidup Lactobacillus plantarum Mar8 dan A17 Lactobacillus signifikan. yaitu 8,59 log10 cfu / g dan 7,28 log10cfu / g. Penyimpanan setiap sampel adalah variasi, tetapi LactobacillusA17 dengan dekstrin 10% dapat disimpan sampai 72,02 hari dan tingkat mortalitas adalah 0,00141 cfu / g / jam, sedangkan L.plantarum Mar8 bisa menjadi toko hanya sampai 14,63 hari dan tingkat mortalitas adalah 0,00867 cfu / g / jam. Jadi, perawatan ini dengan menambahkan jus markisa dengan dekstrin 10% dapat direkomendasikan sebagai probiotik dalam bentuk bubuk kata kunci: Lactobacillus, enkapsulasi, dekstrin, gairah jus buah AbstractThe liquid probiotic is not efficient in stability and packaging. The powder probiotic could be kept longer and stability in quality. Powder could be made by encapsulation method while the concentration of coating is very important. The flavor of the powder like passion fruit juice was needed to get the nice taste in drinking. This research used L. plantarum Mar 8 and Lactobacillus A17 by adding passion fruit juice and dextrin with several concentration like 2%, 5%, 10% and 15%. Then encapsulated by using spray dryer at 125oC. The powder of encapsulation stored for 2 weeks at 4oC, viability test had been done to the powder before and after encapsulation after stored for 2 weeks at4oC. After encapsulation that the highest weight on the treatment by using passion fruit juice of Lactobacillus A17 with 15% dextrin was 5649.6 mg and followed by L. plantarum Mar8 was 5400 mg. The viability after encapsulation for each dextrin concentration was significant, except at 10% concentration was not significant, but in storage for two weeks storage after encapsulation the viability of Lactobacillus plantarum Mar8 and  Lactobacillus A17 was  ignificant. ie, 8.59 log10 cfu/g and 7.28 log10cfu/g. The storage of each sample were variation, but Lactobacillus A17 with 10% dextrin could be stored until 72.02 days and the mortality rate was 0.00141 cfu/g/hour, while L.plantarum Mar8 could be store only until 14.63 days and the mortality rate was 0,00867 cfu/g/hour. So,this treatment by adding passion fruit juice with 10% dextrin could be recommended as probiotic in powder forms key words : Lactobacillus, encapsulation, dextrin, passion fruit juice
PREPARASI DEINOCOCCUS RADIODURANS DAN KHAMIR DALAM MATERIAL KECAP L-DRYING SEBAGAI BAHAN UJI PROFISIENSI Yulinery, Titin; M.Dewi, Ratih
Jurnal Teknologi Lingkungan Vol. 13 No. 1 (2012)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jtl.v13i1.1409

Abstract

Tes kemampuan adalah salah satu kegiatan penting dalam pengendalian mutu dan jaminan kualitas mikrobiologi laboratorium untuk mengukur kompetensi analis dan analisis uji profisiensi membutuhkan persiapan Model mikroorganisme adalah kualitas standar dan validitas. Mikrobiologi uji kualitas produk kedelai utama diarahkan pada kehadiran Saccharomyces cerevisiae ragi (S. cerevisiae), S. Bailli, S. rouxii dankontaminan bakteri seperti Bacillus dan Deinococcus. Jenis ragi dan bakteri yang terlibat dalam proses dan dapat menjadi salah satu parameter kualitas penting dalam persiapan yang dihasilkan. Jumlah dan viabilitas bakteri dan ragi menjadi parameter utama dalam proses persiapan bahan uji. Jumlah tersebut adalah jumlah minimum yang berlaku dapat dianalisis. Jumlah ini harus dibawah 10 CFU diperlukan untuk menunjukkan tingkat hygienitas proses dan tingkat minimal kontaminasi. Viabilitas bakteri dan bahan tes ragi persiapan untuk tes kemahiran kecap yang diawetkan dengan L-pengeringan adalah teknik Deinococcus radiodurans (D. radiodurans) 16 tahun, 58 tahun S. cerevisiae, dan S. roxii 13 tahun. kata kunci: Viabilitas, Deinococcus, khamir, L-pengeringan, Proficiency AbstractProficiency test is one of the important activities in quality control and quality assurance microbiology laboratory for measuring the competence of analysts and analysis Proficiency test requires a model microorganism preparations are standardized quality and validity. Microbiological test of the quality of the main soy products aimed at thepresence of yeast Saccharomyces cerevisiae (S. cerevisiae), S. bailli, S. rouxii and bacterial contaminants such as Bacillus and Deinococcus. Types of yeasts and bacteria involved in the process and can be one of the important quality parameters in the preparation produced. The number and viability of bacteria and yeasts become themain parameters in the process of test preparation materials. The amount in question is the minimum number that is valid can be analyzed. This amount must be below 10 CFU required to indicate the level of hygienitas process and the minimum level of contamination. Viability of bacteria and yeast test preparation materials for proficiencytest of soy sauce that preserved by L-drying technique is Deinococcus radiodurans ( D. radiodurans ) 16 years, 58 years S. cerevisiae, and S. roxii 13 years. key words : Viability, Deinococcus, Khamir, L-drying, Proficiency
Characterization of Cellulase Enzyme Produced by Two Selected Strains of Streptomyces Macrosporeus Isolated from Soil in Indonesia Soeka, Yati; Suharna, Nandang; Triana, Evi; Yulinery, Titin
Makara Journal of Science
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study was conducted to characterize the cellulase enzymes produced by Streptomyces macrosporeus BB 32 and S.microspores KRC 21. D, which were isolated from Tanjung Pendam, Belitung Island, and from Cibodas Botanical Gar-den, Indonesia, respectively. The optimal activity of the enzymes was analyzed using parameters such as incubationtime, pH, temperature, carboxymethylcellulose (CMC) concentration. The effect of the addition of some metal ions asactivators or inhibitors was also analyzed spectrophotometrically at λ 540 nm. Results demonstrated that the activity of the cellulase enzymes of S. macrosporeus BB 32 and S. macrosporeus KRC 21 D reached the optimum level after 2 and5 days of incubation and at pH values of 8.0 and 6.0, temperatures of 35 °C and 50 °C, and CMC concentrations of 1.75% and 2%, respectively. S. macrosporeus BB 32 cellulase was activated by the cations CuCl2, MgCl2, and ZnCl2but inhibited by NaCl and CoCl2, reducing its activity. The cellulase of S. macrosporeusKRC 21.D was activated bythe cation NaCl and by the divalent cations CoCl2, CuCl2, MgCl2, and ZnCl2.S. macrosporeus BB 32 was deposited at the Indonesian Culture Collection with the collection number InaCC A144.