Penelitian ini bertujuan untuk (1) mendeskripsikan biografi pelukis Nusa Penida, (2) mendeskripsikan konsep berkarya pelukis Nusa Penida, (3) mendeskripsikan tema dan gaya karya pelukis Nusa Penida. Sumber data berasal dari hasil observasi, wawancara langsung kepada pelukis Nusa Penida dan praktisi seni, dokumentasi, buku, katalog pameran dan koran. Hasil temuan dalam penelitian ini adalah: 1) biografi pelukis Nusa Penida meliputi, I Made Sumerta, pelukis yang menetap di Nusa Penida, lahir di Nusa Penida, 28 September 1974, I Putu Sudiana, pelukis yang menetap di luar Nusa Penida, lahir di Nusa Penida, 30 Desember 1972, Michael John Apleton, pelukis yang menetap di Nusa Penida, lahir di Inggris, 19 Mei 1951. 2) konsep berkarya I Made Sumerta mengusung konsep Tri Hita Karana dan gerak tubuh manusia, I Putu Sudiana mengusung konsep spiritual alam Nusa Penida dan kehidupan sosial, Michael John Appleton mengusung konsep lingkungan alam. 3) tema dan gaya yang diusung oleh I Made Sumerta yaitu tema keagamaan dan tema lingkungan alam, dengan gaya ekspresif, surealis dan gabungan antara ekspresif dan abstraksi, I Putu Sudiana mengusung tema spiritual dan sosial budaya dengan gaya abstrak dan abstraksi, Michael John Appleton mengusung tema lingkungan alam dan potret, dengan gaya realis dan naturalis.Kata Kunci : Pelukis Nusa Penida, Biografi, Tema dan Gaya The purpose of this study is to (1) describe the biography of Nusa Penida artist, (2) describe the concept creations of Nusa Penida artist, (3) describe the theme and style the creations of Nusa Penida artists. The resources of data is taken from the result of observation, direct interview with the Nusa Penida’s artists and art practitioners, documentation, books, exhibitions catalog, and magazine. The finding results of this study is: 1) biography of Nusa Penida artist include, I Made Sumerta, painters in Nusa Penida, was born in Nusa Penida, on 28 September 1974, I Putu Sudiana, painter in beyond of Nusa Penida, was born in Nusa Penida on 30 December 1972, Michael John Appleton, painter in Nusa Penida which born in English 19 May 1951. 2) concept creations of I Made Sumerta carries concept of Tri Hita Karana and movement body of human, I Putu Sudiana carries concept of spiritual nature of Nusa Penida and social lifes, Michael John Appleton carries concept of nature environment. 3) theme and style the creations of I Made Sumerta carries theme of religious and theme of nature environment, with style expressive, surrealist and combination between expressive and abstract, I Putu Sudiana carry theme spiritual and social culture with style abstract and abstraction, Michael John Appleton carry theme nature environment and portrait with style realist and naturalist.keyword : Nusa Penida artist, biography, theme and style