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All Journal Journal Of Vocational Health Studies Proceedings of the International Conference on Nursing and Health Sciences Media Farmasi Indonesia Indonesian Journal of Medical and Pharmaceutical Science
Erikania, Susanti
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Biochemistry, Genetics & Molecular Biology Chemistry Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health Other

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Comparison of Antibiofilm Activities of Green Coffee Beans (Coffea Canephora P.) and Roasted Robusta Coffee (Coffea Canephora L.) Against Staphylococcus Aureus ATCC 25923 Wicahyo, Septian Maulid; Wardani, Tatiana Siska; Erikania, Susanti
Proceedings of the International Conference on Nursing and Health Sciences Vol 5 No 1 (2024): January-June 2024
Publisher : Global Health Science Group

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/picnhs.v5i1.2794

Abstract

Biofilm is a collection of microbial cells that are irreversibly attached to a surface and encased in an EPS matrix (Extracellular Polymeric Substances). One of the infectious bacteria that produce biofilms is Staphylococcus aureus. Coffee contains compounds that are responsible for antibacterial activity, including alkaloids, flavonoids, phenolics, terpenoids/steroids and saponins among others. The purpose of this study was to determine the activity of the antibiofilm between Green Coffee (Coffea canephora P.) and Robusta Coffee (Coffea canephora L.) against Staphylococcus aureus bacteria. Extraction of green coffee beans and robusta coffee beans was carried out by maceration method, fractionation was carried out by liquid-liquid extraction method using water, ethyl acetate and n-hexane as solvents. Inhibitory activity and biofilm degradation were carried out using the crystal violet staining method which was read at a wavelength of 595 nm. Obtained inhibition and degradation were analyzed using the ANOVA statistical test. The method for testing anti-biofilm activity was determined by testing the inhibition of biofilm formation and biofilm degradation using extracts and fractions robusta green coffee bean (Coffea canephora P.) and roasted robusta coffee beans (Coffea canephora L.) at various concentrations of 2, 4, 8 and 16 mg/ml. Robusta roasted coffee bean extract has the greatest anti-biofilm inhibition effectiveness with IC50 biofilm inhibition of 2.13 ppm. The ethyl acetate fraction from roasted robusta coffee beans has the greatest effectiveness in destroying (degrading) anti-biofilms with the EC50 biofilm degradation of 1.93 ppm.
Aktivitas Antioksidan Ekstrak, Fraksi dan Sub Fraksi Ekstrak Etanol Daun Manggis (Garcinea mangostana) dan Kuantifikasi Senyawa Aktif Dalam Kelompok Sub Fraksi Secara Densitometri erikania, susanti; Silfiana, Dita; Kurniawati, Nurrizka; Kristyanti, Yulia
Media Farmasi Indonesia Vol. 18 No. 2 (2023): Media Farmasi Indonesia
Publisher : SEKOLAH TINGGI ILMU FARMASI YAYASAN PHARMASI SEMARANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53359/mfi.v18i2.220

Abstract

Tanaman manggis (Garcinia mangostana L.) banyak dilaporkan memiliki aktivitas farmakologi seperti antimikroba, antikanker, antiinflamasi, antidiabetes, dan antioksidan. Antioksidan merupakan suatu senyawa yang dapat menyerap atau menetralisir radikal bebas sehingga mampu mencegah penyakit degenaratif. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan ekstrak, fraksi dan sub fraksi ekstrak etanol daun manggis beserta kuantifikasi senyawa aktifnya. Ekstraksi dilakukan secara maserasi menggunakan etanol 96%. Ekstrak hasil maserasi di partisi secara ECC. Ekstrak dan fraksi hasil ECC dilakukan uji antioksidan menggunakan metode DPPH. Hasil antioksidan tertinggi selanjutnya difraksinasi secara KCV (kromatografi cair vakum). Fraksi dimonitoring menggunakan kromatografi lapis tipis (KLT) menghasilkan 3 kelompok sub fraksi yaitu A, B dan C. Pengujian antioksidan dilanjutkan pada 3 kelompok sub fraksi dengan metode DPPH. Kuantifikasi senyawa aktif dalam kelompok sub fraksi menggunakan metode KLT densitometri dengan fase diam silica gel F254 dan fase gerak etil asetat : toluen : metanol : asam format (6:6:3:2). Analisis data dan statistik menggunakan SPSS One way Anova. Hasil penelitian menunjukkan fraksi etanol hasil ECC memiliki nilai IC50 tertinggi yaitu 3,21 ppm dibandingkan ekstrak etanol 4,00 ppm, fraksi n heksan 4,59 ppm dan fraksi etil asetat 3,56 ppm. Kelompok sub fraksi C memiliki aktivitas antioksidan tertinggi dengan nilai IC50 yaitu 18,09 ppm dibandingkan A sebesar 311,81 ppm dan B sebesar 60,52 ppm. Kuantifikasi kelompok sub fraksi secara KLT densitometri diperoleh nilai kadar yaitu C sebesar 1,92 µg/ml, B sebesar 0,80 µg/ml dan A sebesar 1,84 µg/ml. Hasil uji SPSS One way Anova menunjukkan nilai signifikan (p<0,05) yang berarti terdapat perbedaan yang signifikan antara kelompok sub fraksi A, B, dan C. Sehingga dapat disimpulkan bahwa kelompok sub fraksi C memiliki aktivitas antioksidan tertinggi dengan IC50 sebesar 19,09 ppm dan memiliki kadar senyawa aktif tertinggi yaitu 1,92 µg/ml.
ANTI-BACTERIAL ACTIVITY TEST OF ETHANOL EXTRACTS AND ETHLACETATE FRACTION FROM THE EXTRACT OF JATROPHA CURCAS L. LEAVES AGAINST STAPHYLOCOCCUS AUREUS Wulandari, Sekar; Erikania, Susanti; Maritha, Vevi
Journal of Vocational Health Studies Vol. 5 No. 1 (2021): July 2021 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V5.I1.2021.31-38

Abstract

Background: Staphylococcus aureus is a gram-positive bacteria that can cause infection. One of the plants that has antibacterial activity is jatropha leaves which contain flavonoids, saponins, tannins, alkaloids, steroids and polyphenols. Purpose: To determine the antibacterial activity of ethanol extract and ethyl acetate fraction with concentrations of 30%, 60% and 100% against Staphylococcus aureus bacteria. Method: The method for extracting jatropha leaves is maceration with 96% ethanol solvent and the fractionation method, namely liquid-liquid fractionation with ethyl acetate solvent. Antibacterial activity test was carried out in vitro with the disc diffusion method and compared the mean zone of inhibition of each treatment with a positive control (gentamicin 10 μg). Result: The results showed that the ethanol extract and ethyl acetate fraction of jatropha leaves had a strong resistance response, while the positive control gave a very strong inhibitory response to the growth of S. aureus bacteria. Based on the one way ANOVA test, ethanol extract and ethyl acetate fraction showed a significant difference from each treatment with a significant value (P=<0.05). Conclusion: The ethanol extract of Jatropha leaves can inhibit the growth of S. aureus bacteria at a concentration of 100% (18.28 ± 0.50 mm), 100% concentration of ethyl acetate fraction (15.10 ± 0.12 mm). The ethanol extract provided the best inhibition power, namely 18.28 ± 0.50 mm and a positive control 21.82 ± 0.092 mm.
ANTIBACTERIAL ACTIVITY OF MANGOSTEEN BARK FRACTION (GARCINIA MANGOSTANA L.) SALMONELLA TYPHI ATCC 13311 Anggraini, Salmah Wilujeng; Erikania, Susanti; Maritha, Vevi
Journal of Vocational Health Studies Vol. 5 No. 3 (2022): March 2022 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V5.I3.2022.139-145

Abstract

Background: Typhoid fever is caused by Salmonella typhi bacterial infection which spreads via the faecal-oral route and has epidemic potential. Salmonella typhi is a Gram-negative bacterium in the Enterobacteriaceae family. Currently, the use of natural ingredients can be an alternative treatment for typhoid fever, one of which is the mangosteen stem skin (Garcinia mangostana L.). Purpose: To determine the antibacterial activity of mangosteen stem skin fraction against Salmonella typhi ATCC 13311. Method:The maceration method was selected using methanol solvent and fractionation by ECC using ethyl acetate and n-hexane as solvents. This study used five treatments (fraction with a concentration of 20%, 40%, 80%, chloramphenicol 30µg as a positive control, and DMSO as a negative control) which tested their antibacterial activity against Salmonella typhi ATCC 13311 by disc diffusion. Result: The results showed that the ethyl acetate fraction of mangosteen stem skin had the greatest average inhibition zone, that is 13.86 ± 0.72 mm compared to the n-hexane fraction of mangosteen stem skin with an inhibition zone of 10.43 ± 0.73 mm. The data obtained were then analyzed with One-way ANOVA. Conclusion: The results obtained were p=0.000 (sig <0.05), indicating that there was a significant difference between the ethyl acetate fraction and the n-hexane fraction with 20%, 60%, and 80% concentrations with positive control
Validasi Metode Penetapan Kadar Etanol pada Parfum Isi Ulang yang Dijual di Kota Madiun menggunakan Metode Kromatografi Gas Detektor FID (Flame Ionization Detection) Wigyaningrum, Amelia Ike; Kurniawati, Nurrizka; Erikania, Susanti
Indonesian Journal of Medical and Pharmaceutical Science Vol. 3 No. 2 (2024)
Publisher : Sultan Agung Islamic University of Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/ijmps.v3i2.202

Abstract

Background: Perfume and fragrance oils are among the thousands of industrial products that are in demand by the public. The general public uses fragrance oils as body fresheners, for clothing and for rooms. Perfume is a mixture of essential oils and aromatic compounds, binders and solvents used to provide fragrance to the human body, objects or rooms. The purpose of this study was to analyze the ethanol content in refill perfume products. Method: This study used gas chromatography, gas chromatography is a dynamic method for the separation and detection of volatile compounds in a mixture. Gas chromatography can be used to determine the concentration of ethanol. This method has been improved and shows a significant increase in determining the concentration of ethanol. The tests carried out include qualitative and quantitative tests. Results: The results of the qualitative test showed that all perfume samples were positive for ethanol. While the results of the quantitative test to determine the ethanol content in perfume samples showed results of 1.9%; 2.04%; 2.03%; 1.83% and 1.84%. According to BPOM, the safe ethanol content in perfume is 5% calculated as % of ethanol and isopropanol. Conclusion: The FID detector gas chromatography method is efficiently used in determining the ethanol content in perfume. The results of the calculation of the content values contained in sequence from samples 1,2,3,4, and 5 are 1.9%; 1.49%; 2.03%; 1.83% and 1.84%. All refill perfume samples sold in perfume shops in Madiun City meet BPOM standards.
Co-Authors Anggraini, Salmah Wilujeng Kristyanti, Yulia Kurniawati, Nurrizka Maritha, Vevi Sekar Wulandari Silfiana, Dita Wardani, Tatiana Siska Wicahyo, Septian Maulid Wigyaningrum, Amelia Ike