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All Journal METAMORFOSA Journal of Biological Sciences Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Journal of Health Community Service BIOGENESIS
Endry Nugroho Prasetyo
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Other

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Comparison of Three DNA Isolation Methods of Aspergillus Niger Adyan Donastin; Maharani Pertiwi Koentjoro; Muhamad Taufik Hidayat; Endry Nugroho Prasetyo
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p07

Abstract

Polymerase Chain Reaction (PCR) is a technique that is applied to detect and test the presence of genetic material from pathogenic fungi. This method was further developed because it has a high sensitivity compared to the culture method in detecting the presence of pathogenic fungi. To perform sensitive, specific and reliable PCR-based assays, the availability of pure DNA as well as an easy-to-perform DNA extraction protocol is essential. Currently, existing protocols for DNA extraction generally require specialized kits and with the addition of enzymes. Therefore, this study was conducted to compare the quantity and quality of Aspergillus niger DNA isolation with three different methods. The stages of the research carried out included pure A. niger culture and total DNA isolation using three methods, namely the protocol according to the Wizard® Genomic DNA Purification Kit (P1) instructions, Modified Wizard® Genomic DNA Purification Kit (P2) and Monarch Genomic DNA Purification Kit NEB (P3). The results of the evaluation of DNA isolation using a spectrophotometer at a wavelength of A260/280 nm showed a ratio of 1.4, 1.8, and 1.7 at P1 and P2, and P3 respectively. The quantity obtained ranged from 210 to 305 ng/µL. The total DNA results obtained were then used for PCR testing of ITS ribosomal DNA fragments using ITS1 and ITS4 primers. Electrophorosis observation of PCR results showed that all samples produced bands with a length of 500 bp. The results of DNA isolation in the P1 method did not show bands on the agarose gel, but could be seen in the PCR product. Methods P2 and P3 showed DNA that had good quality and quality. The P2 and P3 methods use a cell destruction technique with liquid nitrogen and a combination of addition of proteinase K. The obtained protocol is expected to provide fast and good method information in the isolation of total DNA from A. niger. Keyword: Aspergillus niger, total DNA isolation, ITS1, ITS4
Biogrouting: Produksi Urease dari Bakteri Laut (Oceanobacillus sp.) Pengendap Karbonat Sidratu Ainiyah Puspita Lisdiyanti Maharani Pertiwi; Endry Nugroho Prasetyo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 1, No 1 (2016): February 2016
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v1i1.707

Abstract

Biogrouting adalah teknologi yang mensimulasi proses diagenesis yaitu transformasi butiran pasir menjadi batuan pasir (calcarinite/sandstone).  Permasalahan penelitian ini bagaimana mengoptimasi produk urease dengan melakukan uji aktifitas, mengisolasi, mempurifikasi dan mengkarakterisasi serta mengaplikasikannya sebagai material grout. Uji aktifitas dan optimasi dilakukan dengan menumbuhkan isolat Oceanobacillus sp. pada dua variasi medium (B4 urea dan B4 urin), lima variasi pH (4-8) dan dua variasi suhu (25°C dan 29°C). Hasil uji aktifitas dan optimasi selanjutnya dipurifikasi menggunakan ammonium sulfat dan dicari titik isoelektriknya. Kemudian hasil protein presipitat dikarakterisasi menggunakan SDS-PAGE. Berdasarkan hasil penelitian diketahui bahwa aktifitas urease paling tinggi adalah 203,32 unit/ml. Urease optimal dihasilkan pada isolat yang ditumbuhkan pada B4 urea pada pH 7 suhu 25°C. Berat molekul urease yang dikarakterisasi menggunakan SDS-PAGE adalah 440 kDa, sedangkan titik isoelektriknya pada pH 6. Urease dapat dijadikan material  grout  karena memiliki kemampuan untuk melakukan sementasi pada aplikasi sederhana biogrouting.
PENGARUH MASA SIMPAN DAN JENIS PENGIKAT GRAFTING TERHADAP KEBERHASILAN GRAFTING SIRSAK (Annona muricata) VARIETAS RATU Tri Wahyu Hidayat; Ali Mustafa Sidauruk; Rico Hutama Sulistiyo; Buana Susilo; Lengga Nurullah Dalimartha; Eko Chandra Wiguna; Isdiantoni Isdiantoni; Maharani Pertiwi Koentjoro; Endry Nugroho Prasetyo
Biogenesis Vol 14, No 2 (2018)
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (548.916 KB) | DOI: 10.31258/biogenesis.14.2.7-12

Abstract

ABSTRACTIn nursery technology, the storage of scions always requires approriate optimization of shelf life and good packing This study aims to determine the effect of shelf life and storage materials on viability of soursop (Annona muricata) var. Ratu grafting by applying randomized factorial design with 2 factors: storage time and type of packing materials, with 3 times repeatation. The duration of storage time factor consist of 5 levels: 0 day, 3 days, 5 days, 1 week and 2 weeks meanwhile packing materials factor consist of 2 levels: parafilm and polyethylene. The highest survival rate was obtained at 0 day shelf life time and parafilm as storage material, whereas the lowest one was shown at 2 weeks storage with polyethylene as storage material.Keywords: Scion, Grafting, Storage, Packing Material, Soursop
Hypertension Prevention Education Through Training in Making Herbal Teas in Talango Village, Talango District, Sumenep Regency Maharani Pertiwi K; Bastiana Bastiana; Asma Rubiah Rosyadahan; Suprapto Maat; Isdiantoni Isdiantoni; Endry Nugroho Prasetyo
Journal of Health Community Service Vol. 2 No. 2 (2022): Journal of Health Community Service: 2022 Mei
Publisher : UNUSA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/jhcs.v2i2.2758

Abstract

Talango Village is one of the villages located in Talango District, Sumenep Regency. The people of this village make a living mainly as fishermen and farmers. One of the habits carried out by fishers and farmers in between their activities is smoking. The nicotine in cigarette smoke can increase blood pressure and heart rate, narrow arteries, harden their walls, and make blood more likely to clot. This condition can increase the risk of hypertension or high blood pressure. Based on interviews and surveys with partners Yayasan Jala Tani Pertiwi, education about hypertension prevention needs to be carried out to prevent hypertension in fishers and hypertension in Talango Village. This problem has prompted a service team to design educational activities to prevent hypertension by manufacturing herbal teas. This activity is structured through the first step, namely the preparation stage (compilation of training modules, participant selection, and evaluation media creation), the second stage (the process of implementing activities with pre-/post-test and discussion), and the third stage (evaluating the results of the training). A total of 20 farmers and fishers who are members of the Jala Tani Pertiwi Foundation were given education about hypertension, then continued with training to make anti-hypertensive herbal teas. The evaluation results showed an increase in participants' understanding of the material provided by 34%. Participants are known to have been able to understand and make herbal teas made from Moringa leaves, jasmine flowers, lemongrass leaves, and roselle flowers. Furthermore, to increase the participants' interest in this activity, the service team also taught them how to make tea packaging and how to sell it.
Co-Authors Adyan Donastin Ali Mustafa Sidauruk Asma Rubiah Rosyadahan Bastiana Bermawi Buana Susilo Eko Chandra Wiguna Isdiantoni Isdiantoni Lengga Nurullah Dalimartha Maharani Pertiwi K Maharani Pertiwi Koentjoro Muhammad Taufiq Hidayat Rico Hutama Sulistiyo Sidratu Ainiyah Puspita Lisdiyanti Maharani Pertiwi Suprapto Maat Tri Wahyu Hidayat