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All Journal Jurnal Biomedika KHIDMAH Jurnal Pengabdian Masyarakat Jurnal Laboratorium Prima
Mustika Sari Hutabarat
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Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Detection of LytA Genes in Streptococcus pneumoniae Isolated from sputum pneumonia patients Mustika Sari Hutabarat; Firdaus Hamid; Irawaty Djaharuddin; Alfian Zainuddin; Rossana Agus; Muhammad Nassrum Massi
Biomedika Vol 13 No 1 (2020): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/biomedika.v13i1.718

Abstract

Streptococcus pneumoniae (pneumococcus) is a Gram-positive facultative anaerobic bacterium that is a major cause of morbidity and mortality worldwide. But the lack of reporting of disease by this bacterium in Indonesia, one of the causes is because the diagnosis of pneumococcal infection is often clinically not typical and conventional methods which are still the standard gold method often give false-negative results. So the purpose of this study was to evaluate the performance of culture and molecular diagnostic methods using the Polymerase Chain Reaction (PCR) technique in detecting Streptococcus pneumoniae in sputum clinical samples using the Autolysin (LytA) gene which is a virulence factor of this bacterium. 57 isolates from 60 samples were confirmed as Streptococcus sp through microscopic identification, culture, and biochemical tests. Then the sensitivity test with an optochin test of 9 (9%) compared the results descriptively with the PCR technique using the Autolysin A (LytA) gene which was obtained more sensitive by 15 (25%).
Perbedaan Jumlah Leukosit yang Dihomogenisasi Sekunder Sebanyak 2 dan 8 Kali setelah Didiamkan Selama 30 Menit Kartika Englishiana; Rosnita Sebayang; Mustika Sari Hutabarat
Jurnal Laboratorium Prima Vol. 1 No. 1 (2023): Jurnal Laboratorium Prima
Publisher : Fakultas ilmu Kesehatan Universitas Katolik Musi Charitas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32524/jlp.v1i1.1050

Abstract

Background: Leukocytes are one of the hematological examinations. Blood collected in K2EDTA tubes must be primary homogenized and then examined. However, sometimes in the laboratory, the examination is not carried out immediately after blood collection so that if it is allowed to stand for some time, it must be secondary homogenized so that it does not form a precipitate and mixes well. Objective: To determine whether there is a difference in the number of leukocytes that are secondary homogenized 2 and 8 times after standing for 30 minutes. Methods: This type of research is a cross-sectional study. Blood was examined using the Sysmex XP- 100 tool with the impedance method in the UKMC FIKES Clinical Chemistry laboratory. The samples in this study were students from the DIV Medical Laboratory Technology Study Program level I and IV by total sampling. Data were analyzed using the Paired Samples T Test. Results: Secondary homogenized leukocyte count examination 2 times obtained an average result of 7.41 x 103 /µl, and secondary homogenized leukocyte count 8 times obtained an average result of 7.43 x 103 /µl. These results show there is no significant difference between secondary homogenization 2 and 8 times with p (sig) = 0.852 (> 0.005). Conclusion: Based on this study, it can be concluded that there is no difference in the results of secondary homogenized leukocyte counts 2 and 8 times. Suggestion: Secondary homogenization is sufficient for 2 times after the sample has been allowed to stand for 30 minutes.
PEMERIKSAAN KESEHATAN MENUJU LANSIA SEHAT DAN BUGAR mustika sari hutabarat; Novita Anggraini; Mustika Sari; Lidwina Septie; Aprida Manurung; Petronela Sihotang; Oktasari Oktasari; Dewi Rindi; Yohana Arvera
Khidmah Vol 3 No 1 (2021): Khidmah
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/khidmah.v3i1.344

Abstract

Kegiatan pengabdian kepada masyarakat khususnya di bidang kesehatan merupakan kegiatan yang perlu dilakukan secara berkala terutama bagi para usia lanjut (lansia) untuk menskrining Penyakit Tidak Menular (PTM). Kegiaan ini bertujuan meningkatkan status kesehatan para lansia melalui pemantauan tekanan darah dan kolesnterol, memberikan motivasi untuk melakukan aktivitas fisik sehingga diharapkan mengurangi perilaku sedentary yang dapat memicu terjadinya sindrom metabolik yang rentan terjadi pada lansia. Kegiatan pemeriksaan dan penyuluhan ini dilakukan kepada 31 orang lansia di Rumah Panti Jompo Werdha Sumarah Sukamoro Kota Palembang. Sebagai hasilnya diperoeh 17 (orang dengan kadar koesterol norma (<200 mg/dL), 23 orang (76.6%) dengan hasil pemeriksaan kolesterol diatas nilai normal (>200 mg/dL). Maka dari hasi ini dapat dilihat bahwa rata-rata warga panti jompoh memiliki faktor resiko hiperkolesterol, oleh karena itu diharapkan warga panti jompo dapat mengubah pola hidup yang dimulai dari pola makan dan mengatur aktivitas fisik secara teratur seperti mulai rutin melakukan jalan kaki serta aktivitas fisik lainnya untuk mencegah terjadinya komplikasi penyakit generatif.
Co-Authors Alfian Zainuddin Aprida Manurung Dewi Rindi Firdaus Hamid Irawaty Djaharuddin Kartika Englishiana Lidwina Septie Muhammad Nassrum Massi Mustika Sari Novita Anggraini Oktasari Oktasari Petronela Sihotang Rosnita Sebayang Rossana Agus Yohana Arvera