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Journal : Journal Of Widya Medika Junior

DIFFERENCES OF MASS AND DIAMETER MUSCLE RATTUS NORVEGICUS IN GIVING KETOGENIC DIETS AND EXERCISE Danika, Andri; Dewi, Dewa AL; Sindrawati, Sindrawati
JOURNAL OF WIDYA MEDIKA JUNIOR Vol 3, No 3 (2021): July
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwmj.v3i3.3343

Abstract

Introduction: Saturated ketogenic diet without exercise can lead to muscle mass decrease and disturb body metabolism. Therefore, the researcher is interested in discovering the differences between a saturated and unsaturated ketogenic diet with exercise to muscle mass and diameter. Purpose: This study aimed to analyze the difference in muscle mass and diameter of Rattus norvegicus, which were given a ketogenic diet and exercise. Method: This experimental research used Rattus norvegicus that had been adapted for two weeks. Rats are divided into five groups; control (K), a saturated ketogenic diet (P1), a saturated ketogenic diet with exercise (P2), an unsaturated ketogenic diet (P3), and anunsaturated ketogenic diet with exercise (P4). Upright running wheel exercise was given to the group with exercise for 10 minutes daily. The ketogenic diet was given with oral gavage. Muscle mass was examined in the second and fourth weeks. Muscle diameter was examined before treatment, second week, and fourth week. Results: There are differences in mass and diameter muscle Rattus norvegicus that givenketogenic diet and exercise (p=0,000). In second week, there are significant increase inmuscle diameter for saturated ketogenic diet without exercise (P1(p=0,000)) and significantdecrease in muscle diameter for three other group (P2(p=0,000), P3(p=0,001), P4(p=0,000)). In the fourth week, there is a significant increase in muscle diameter for an unsaturated ketogenic diet with exercise (P4). Conclusion: An unsaturated ketogenic diet with exercise could increase the mass anddiameter of quadriceps muscle at the end of this research. 
ANTIBACTERIAL TEST OF SQUEEZED WATER OF KATUK LEAF ON STREPTOCOCCUS PYOGENES Novitayanti, Ni Putu S; Rahayu, Titien; Sindrawati, Sindrawati
JOURNAL OF WIDYA MEDIKA JUNIOR Vol 4, No 1 (2022): January
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwmj.v4i1.3576

Abstract

Background: Many infectious diseases still can be found in developing countries, including Indonesia. The common type of infection is upper respiratory tract infection (URTI) in which one of the diseases is pharyngitis. About 5% - 40% of pharyngitis cases are caused by Streptococcus pyogenes. One of the treatments for bacterial infection is antibiotics, but the increasing number of antibiotic resistance causes the need to find other alternatives such as herbal plants for the treatment. The community of Bali, particularly in Singaraja, often consume the squeezed water of Katuk leaf (Sauropus androgynus (L.) Merr) to treat pharyngitis.Objectives: This study aims to determine the antibacterial effect of squeezed water of Katuk leaf on Streptococcus pyogenes by measuring the value of the minimum inhibitory concentration (MIC) and the value of the minimum bactericidal concentration (MBC).Methods: The squeezed water of Katuk leaf was obtained through the squeezing method. The antibacterial activity test used the microdilution method of 96 well plates, then the absorbance value was read using a spectrophotometer to determine the MIC value, and streaking was performed to the blood agar strengthened by the results of the spectrophotometric readings to determine the value of MBC.Results: The results showed that the 300 grams of squeezed water of Katuk leaf had MIC values at a concentration of 10% and MBC values at a concentration of 20%.Conclusion: It can be concluded that the squeezed water of Katuk leaf has an antibacterial effect on Streptococcus pyogenes.Keywords: Antibacterial, Sauropus androgynus (L.) Merr, Streptococcus pyogenes